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Author: Julie Chih-chi Hsu
Publisher:
ISBN: 9780355413892
Category :
Languages : en
Pages : 108
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Book Description
Coherent nonlinear optical microscopy is a rapidly developing field that is widely applicable to the study of biological molecules and systems. These techniques have the inherent advantages of high speed and resolution, three-dimensional focusing, minimal sample damage, and label free chemical selectivity. In this thesis, applications of different vibrationally resonant nonlinear techniques to studying the optical and chemical properties of biological molecules are explored. The noncentrosymmetry sensitive vibrationally-resonant sum frequency generation microscopy is used in a polarization sensitive study of collagen second order nonlinear susceptibility tensor terms. Transient absorption spectroscopy is used to explore the excited state dynamics of of Sepia melanin. Additionally, position dependent phase shifts and changes in spectra in stimulated Raman microscopy are calculated. Optical design of achromatic lenses for implementation in sum frequency generation microscopy is also included.
Author: Julie Chih-chi Hsu
Publisher:
ISBN: 9780355413892
Category :
Languages : en
Pages : 108
Get Book Here
Book Description
Coherent nonlinear optical microscopy is a rapidly developing field that is widely applicable to the study of biological molecules and systems. These techniques have the inherent advantages of high speed and resolution, three-dimensional focusing, minimal sample damage, and label free chemical selectivity. In this thesis, applications of different vibrationally resonant nonlinear techniques to studying the optical and chemical properties of biological molecules are explored. The noncentrosymmetry sensitive vibrationally-resonant sum frequency generation microscopy is used in a polarization sensitive study of collagen second order nonlinear susceptibility tensor terms. Transient absorption spectroscopy is used to explore the excited state dynamics of of Sepia melanin. Additionally, position dependent phase shifts and changes in spectra in stimulated Raman microscopy are calculated. Optical design of achromatic lenses for implementation in sum frequency generation microscopy is also included.
Author: Garth J. Simpson
Publisher: Cambridge University Press
ISBN: 1316797198
Category : Technology & Engineering
Languages : en
Pages : 499
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Book Description
This rigorous yet accessible guide presents a molecular-based description of nonlinear optical polarization analysis of chemical and biological assemblies. It includes discussion of the most common nonlinear optical microscopy and interfacial measurements used for quantitative analysis, specifically second harmonic generation (SHG), two-photon excited fluorescence (2PEF), vibrational sum frequency generation (SFG), and coherent anti-Stokes Raman spectroscopy/stimulated Raman spectroscopy (CARS/SRS). A linear algebra mathematical framework is developed, allowing step-wise systematic connections to be made between the observable measurements and the molecular response. Effects considered include local field corrections, the molecular orientation distribution, rotations between the molecular frame, the local frame and the laboratory frame, and simplifications from molecular and macromolecular symmetry. Specific examples are provided throughout the book, working from the common and relatively simple case studies through to the most general scenarios.
Author: Barry R. Masters
Publisher: Oxford University Press
ISBN: 0198036825
Category : Science
Languages : en
Pages : 895
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Book Description
The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.
Author: Vladislav V. Yakovlev
Publisher: CRC Press
ISBN: 1420068601
Category : Science
Languages : en
Pages : 290
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Book Description
For a host of reasons, nonlinear optical spectroscopy is a valuable tool for biochemical applications where minimally invasive diagnostics is desired. Biochemical Applications of Nonlinear Optical Spectroscopy presents the latest technological advances and offers a perspective on future directions in this important field. Written by an international panel of experts, this volume begins with a comparison of nonlinear optical spectroscopy and x-ray crystallography. The text examines the use of multiphoton fluorescence to study chemical phenomena in the skin, the use of nonlinear optics to enhance traditional optical spectroscopy, and the multimodal approach, which incorporates several spectroscopic techniques in one instrument. Later chapters explore Raman microscopy, third-harmonic generation microscopy, and non-linear Raman microspectroscopy. The text explores the promise of beam shaping and the use of broadband laser pulse generated through continuum generation and an optical pulse shaper. Lastly, the book discusses the effects of spatial beam shaping on the generated nonlinear Raman signals in a tightly focused geometry and provides insight into the extension of nonlinear optical spectroscopy to the nanoscale through the use of plasmonic tip-enhanced arrangement. With novel experimental approaches to this technology expanding day-by-day, the book’s balanced coverage from a wide range of international contributors not only elucidates important achievements, but also outlines future directions in this dynamic and promising field.
Author: Harekrushna Sahoo
Publisher: Springer Nature
ISBN: 9811645507
Category : Science
Languages : en
Pages : 260
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Book Description
This book illustrates the significance of various optical spectroscopy and microscopy techniques, including absorption spectroscopy, fluorescence spectroscopy, infrared spectroscopy, and Raman spectroscopy for deciphering the nature of biological molecules. The content of this book chiefly focuses on (1) the principle, theory, and instrumentation used in different optical spectroscopy techniques, and (2) the application of these techniques in exploring the nature of different biomolecules (e.g., proteins, nucleic acids, enzymes, and carbohydrates). It emphasizes the structural, conformational and dynamic, and kinetic including the changes in biomolecules under a range of conditions. In closing, the book summarizes recent advances in the field of optical spectroscopic and microscopic techniques.
Author:
Publisher: Faraday Discussions
ISBN: 9781782624615
Category : Science
Languages : en
Pages : 0
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Book Description
Since their inception, optical detection and spectroscopy of single molecules have steadily expanded to an amazing variety of disciplines in the natural sciences. Domains such as optical microscopy, quantum optics, nanophotonics and soft matter/ material science have all benefited from the new, "average-free" insights provided by the optical isolation of single molecules, quantum dots, metal nanoparticles, and other nanometre-sized objects. The techniques themselves have also made spectacular progress with developments in super-resolution microscopy, time-resolved measurements, absorption-based detection, combination with mechanical or electrical manipulation and recording, live-cell imaging, and metal nanoparticle-phenomena. Following the Single-Molecule Microscopy and Spectroscopy: Faraday Discussion (September 2015), this book discusses the recent advances and maps out future avenues in the field, covering topics such as quantum optics and plasmonics; probes and sensors for molecular biophysics; super-resolution and imaging of soft and biological matter; and nonlinear optics and coherence in biophysics.
Author: Yin Yeh
Publisher: World Scientific
ISBN: 9813235705
Category : Science
Languages : en
Pages : 290
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Book Description
This lecture volume aims to give students and researchers in this rapidly expanding field of biophotonics an interdisciplinary perspective. Among the primary topics are ultrahigh resolution microscopy, particle tracking, photon correlation spectroscopy, and nonlinear optical methods as used in biological and biomedical research, with a focus on current applications in biophysics and biomedicine.
Author: Alberto Diaspro
Publisher: Springer Science & Business Media
ISBN: 3642151752
Category : Science
Languages : en
Pages : 252
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Book Description
In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.
Author: Christopher Jones
Publisher: Springer Science & Business Media
ISBN: 159259509X
Category : Science
Languages : en
Pages : 260
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Book Description
This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand ing for the biochemist or biologist who needs to collaborate with spe cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to visualize individual molecules or complexes of several molecules. Electron microscopy is the more familiar of these, while scanning tunneling microscopy is a new and rapidly developing tool. Methods for determining the shapes and sizes of molecules in solution are described in Part Two, which includes chapters on X-ray and neutron scattering, light scattering, and ult- centrifugation. Calorimetry, described in Part Three, provides the means to monitor processes involving thermodynamic changes, whether these are intramolecular, such as conformational transition, or the interactions between solutes or between a solute and its sol vent. Part Four is concerned with optical and infrared spectroscopy and describes applications ranging from the measurement of protein concentration by UV absorbance to the analysis of secondary struc ture using circular dichroism and Fourier-transform infrared spec troscopy.
Author: Denis Rousseau
Publisher: Elsevier
ISBN: 0323150217
Category : Science
Languages : en
Pages : 443
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Book Description
Optical Techniques in Biological Research discusses the fundamentals and applications of light scattering, Raman scattering, Fourier transform infrared spectroscopy, nanosecond fluorescence spectroscopy, and circular dichroism. Electron microscopy is also included owing to some of its classical similarity to optical microscopy optical structural and resonance techniques for biological research. The chapters are aimed at a level such that only a general understanding of chemistry and biology is required. The objective is to present material in a way that allows the research worker to assess quickly the applicability, utility, and significance of the specific technique to his or her problem or field of interest. Together with Structural and Resonance Techniques in Biological Research, this book marks the introduction of a new series of volumes, Physical Techniques in Biology and Medicine, which is intended to replace a previous treatise, Physical Techniques in Biological Research.
