MT-PCR - A rapid, reliable and effective tool for assessing toxic ‘algal’ blooms in Victorian water supplies PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download MT-PCR - A rapid, reliable and effective tool for assessing toxic ‘algal’ blooms in Victorian water supplies PDF full book. Access full book title MT-PCR - A rapid, reliable and effective tool for assessing toxic ‘algal’ blooms in Victorian water supplies by Aaron Jex. Download full books in PDF and EPUB format.
Author: Aaron Jex
Publisher: IWA Publishing
ISBN: 1780407556
Category : Science
Languages : en
Pages : 32
Get Book Here
Book Description
This project addressed a need for a sensitive, accurate and reliable testing method to aid assessment of the toxicity of algal blooms and assist water management. Increasingly, diagnostic dilemmas are resolved through the use of DNA-based technologies which often provide high sensitivity and specificity and are efficient both in terms of costs and time. However to date, no such test was available to the Victorian water industries. This project sought to bridge this gap by developing an automated DNA-based diagnostic assay for cyanobacterial bloom assessment blooms in Victorian waters. The assay exceeds expectation in its ability to accurately quantify levels of toxigenic cyanobacteria in bloom samples, retains exceptionally high specificity and sensitivity and each assay out-performs common conventional PCR approaches established in the literature. Four toxigen assays (microcystin, nodularin, cylindrospermopsin and saxitoxin) were designed, tested and optimised. This book is co-published with Water Research Australia Authors: Aaron Jex, Louise Baker and Raechel Littman, University of Melbourne
Author: Aaron Jex
Publisher: IWA Publishing
ISBN: 1780407556
Category : Science
Languages : en
Pages : 32
Get Book Here
Book Description
This project addressed a need for a sensitive, accurate and reliable testing method to aid assessment of the toxicity of algal blooms and assist water management. Increasingly, diagnostic dilemmas are resolved through the use of DNA-based technologies which often provide high sensitivity and specificity and are efficient both in terms of costs and time. However to date, no such test was available to the Victorian water industries. This project sought to bridge this gap by developing an automated DNA-based diagnostic assay for cyanobacterial bloom assessment blooms in Victorian waters. The assay exceeds expectation in its ability to accurately quantify levels of toxigenic cyanobacteria in bloom samples, retains exceptionally high specificity and sensitivity and each assay out-performs common conventional PCR approaches established in the literature. Four toxigen assays (microcystin, nodularin, cylindrospermopsin and saxitoxin) were designed, tested and optimised. This book is co-published with Water Research Australia Authors: Aaron Jex, Louise Baker and Raechel Littman, University of Melbourne
Author:
Publisher:
ISBN: 9781780407562
Category : SCIENCE
Languages : en
Pages :
Get Book Here
Book Description
Author: Paul Monis
Publisher: IWA Publishing
ISBN: 1780407513
Category : Science
Languages : en
Pages : 36
Get Book Here
Book Description
Molecular techniques are emerging as a rapid and powerful approach for microorganism detection. This project reviewed the efficacy of PCR assays for Cryptosporidium, Microcystis, adenovirus and ammonia oxidising bacteria, as well as candidate techniques for DNA extraction and inhibitor removal. The literature review led to an evaluation of DNA extraction kits and reagents for PCR; finalisation of assay formats; the development of PCR controls and Standard Operating Procedures (SOP); analysis of assay robustness using real samples; and completion of an Inter-laboratory trial using the SOP’s developed. This book is co-published with Water Research Australia.
Author:
Publisher:
ISBN: 9780730676379
Category : Water quality biological assessment
Languages : en
Pages : 81
Get Book Here
Book Description
"This guideline provides the necessary background and comprehensive procedures for the use of the rapid bioassessment method and recommended interpretive tools in assessing the environmental conditions of rivers and streams in Victoria. It updates and replaces the Rapid Bioassessment of Victorian Streams (EPA Victoria, 1998, Publication 604.)"--Foreword.
Author: Sharon E. Kroening
Publisher:
ISBN:
Category : Earth sciences
Languages : en
Pages : 122
Get Book Here
Book Description
Author: David J. Suggett
Publisher: Springer Science & Business Media
ISBN: 9048192684
Category : Science
Languages : en
Pages : 332
Get Book Here
Book Description
Measurements of variable chlorophyll fluorescence have revolutionised global research of photosynthetic bacteria, algae and plants and in turn assessment of the status of aquatic ecosystems, a success that has partly been facilitated by the widespread commercialisation of a suite of chlorophyll fluorometers designed for almost every application in lakes, rivers and oceans. Numerous publications have been produced as researchers and assessors have simultaneously sought to optimise protocols and practices for key organisms or water bodies; however, such parallel efforts have led to difficulties in reconciling processes and patterns across the aquatic sciences. This book follows on from the first international conference on “chlorophyll fluorescence in the aquatic sciences” (AQUAFLUO 2007): to bridge the gaps between the concept, measurement and application of chlorophyll fluorescence through the synthesis and integration of current knowledge from leading researchers and assessors as well as instrument manufacturers.
Author:
Publisher:
ISBN:
Category : Sewage
Languages : en
Pages : 378
Get Book Here
Book Description
"This manual contains overview information on treatment technologies, installation practices, and past performance."--Introduction.
Author: Marat Abzalov
Publisher: Springer
ISBN: 3319392646
Category : Science
Languages : en
Pages : 441
Get Book Here
Book Description
This book provides a detailed overview of the operational principles of modern mining geology, which are presented as a good mix of theory and practice, allowing use by a broad range of specialists, from students to lecturers and experienced geologists. The book includes comprehensive descriptions of mining geology techniques, including conventional methods and new approaches. The attributes presented in the book can be used as a reference and as a guide by mining industry specialists developing mining projects and for optimizing mining geology procedures. Applications of the methods are explained using case studies and are facilitated by the computer scripts added to the book as Electronic Supplementary Material.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309086256
Category : Technology & Engineering
Languages : en
Pages : 433
Get Book Here
Book Description
Bioavailability refers to the extent to which humans and ecological receptors are exposed to contaminants in soil or sediment. The concept of bioavailability has recently piqued the interest of the hazardous waste industry as an important consideration in deciding how much waste to clean up. The rationale is that if contaminants in soil and sediment are not bioavailable, then more contaminant mass can be left in place without creating additional risk. A new NRC report notes that the potential for the consideration of bioavailability to influence decision-making is greatest where certain chemical, environmental, and regulatory factors align. The current use of bioavailability in risk assessment and hazardous waste cleanup regulations is demystified, and acceptable tools and models for bioavailability assessment are discussed and ranked according to seven criteria. Finally, the intimate link between bioavailability and bioremediation is explored. The report concludes with suggestions for moving bioavailability forward in the regulatory arena for both soil and sediment cleanup.
Author: Patricia M. Glibert
Publisher: Springer
ISBN: 3319700693
Category : Science
Languages : en
Pages : 460
Get Book Here
Book Description
Harmful algal blooms (HABs) - blooms that cause fish kills, contaminate seafood with toxins, or cause human or ecological health impacts and harm to local economies - are occurring more often, in more places and lasting longer than in past decades. This expansion is primarily the result of human activities, through increased nutrient inputs and various aspects of climate change. The Global Ecology and Oceanography of Harmful Algal Blooms (GEOHAB) programme promoted international collaboration to understand HAB population dynamics in various oceanographic regimes and to improve the prediction of HABs. This volume introduces readers to the overarching framework of the GEOHAB programme, factors contributing to the global expansion of harmful algal blooms, the complexities of HABs in different habitats, and the forward-looking issues to be tackled by the next generation of GEOHAB, GlobalHAB. The programme brought together an international team of contributing scientists and ecosystem managers, and its outcomes will greatly benefit the international research community.
