Molecular Toxicology Protocols PDF Download
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Author: Phouthone Keohavong
Publisher: Springer Science & Business Media
ISBN: 1592598404
Category : Medical
Languages : en
Pages : 478
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Book Description
A collection of cutting-edge techniques for analyzing genotoxic exposure and detecting the resulting biological effects-including endogenous metabolites-up to and including the development of cancer. The authors emphasize analytical methods that can be specifically applied to human populations and patients. Among the applications detailed are the analysis of interactions between such cellular macromolecules as DNA and proteins and chemical and physical agents, the assessment of medically relevant toxicity, and the characterization of genetic alterations induced in transgenic animals by in vivo systems. There are also methods for the analysis of genotoxic exposure during gene expression, of cytotoxicity caused by the induction of apoptosis, of genetic alterations in reporter genes and oncogenes, early (premalignant) detection of altered oncogenes, and of individual variation in biotransformation and DNA repair capacity.
Author: Phouthone Keohavong
Publisher: Springer Science & Business Media
ISBN: 1592598404
Category : Medical
Languages : en
Pages : 478
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Book Description
A collection of cutting-edge techniques for analyzing genotoxic exposure and detecting the resulting biological effects-including endogenous metabolites-up to and including the development of cancer. The authors emphasize analytical methods that can be specifically applied to human populations and patients. Among the applications detailed are the analysis of interactions between such cellular macromolecules as DNA and proteins and chemical and physical agents, the assessment of medically relevant toxicity, and the characterization of genetic alterations induced in transgenic animals by in vivo systems. There are also methods for the analysis of genotoxic exposure during gene expression, of cytotoxicity caused by the induction of apoptosis, of genetic alterations in reporter genes and oncogenes, early (premalignant) detection of altered oncogenes, and of individual variation in biotransformation and DNA repair capacity.
Author: John P. Vanden Heuvel
Publisher: CRC Press
ISBN: 9781439805800
Category : Medical
Languages : en
Pages : 258
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Book Description
Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail: Quantitative reverse transcriptase PCR and methods to examine gene expression Differential display cloning Cloning and library screening by PCR Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes Basic, non-PCR based molecular biology methods PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.
Author: Robert C. Smart
Publisher: John Wiley & Sons
ISBN: 1119042410
Category : Science
Languages : en
Pages : 1060
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Book Description
Written as an advanced text for toxicology students, this book is much more than an introduction and provides in-depth information describing the underlying mechanisms through which toxicants produce their adverse responses. • Links traditional toxicology to modern molecular techniques, important for teaching to graduate courses and professional studies • Uses a didactic approach with basic biological or theoretical background for the methodology presented • Brings together and comprehensively covers a range of dynamic aspects in biochemical and molecular toxicology • Guides student and professional toxicologists in comprehending a broad range of issues, compiled and authored by a diverse group of experts • “A good introductory textbook covering the biochemical toxicology of organic substances and the relevant methodology in some detail.... It offers good value for money and can be recommended as a textbook for appropriate courses” – BTS Newsletter review of the 4th edition
Author: Phouthone Keohavong
Publisher: Humana Press
ISBN: 9781627037389
Category : Medical
Languages : en
Pages : 0
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Book Description
Molecular Toxicology Protocols, Second Edition aims to bring together a series of articles describing validated methods to elucidate specific molecular aspects of toxicology, the emphasis being on the application of molecular methods to genetic toxicology. The volume is divided into ten parts, roughly corresponding to the spectrum of biomarkers intermediate between exposure and disease outcomes as proposed in molecular epidemiology models. Subjects of these new chapters range from preparation of fluid specimens for analysis of cellular inflammatory responses to genotoxic insults to sensitive methods for proteomic analysis and aberrant DNA methylation patterns. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Molecular Toxicology Protocols, Second Edition addresses not only the needs of molecular biologists and toxicologists, but also those of individuals interested in applying molecular methods to clinical applications, such as geneticists, pathologists, biochemists, and epidemiologists.
Author: Nick Plant
Publisher: Taylor & Francis
ISBN: 1000150216
Category : Science
Languages : en
Pages : 164
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Book Description
Molecular Toxicology is a concise introduction to the subject, taking the reader through the theoretical principles of toxicology followed by specific examples. In the first section, the concepts behind possible mechanisms of toxicity are described (e.g. the specific enzyme or receptor system) using examples where appropriate. Following this a series of examples are used to show the extension of concept into the real world, in an organ specific manner. The book concludes with a section outlining toxicity assessment methods, where the impact of molecular biology is having a considerable impact, including DNA microarrays, proteomics and bioinformatics.
Author: James M. Parry
Publisher: Humana Press
ISBN: 9781617794209
Category : Medical
Languages : en
Pages : 433
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Book Description
The evaluation of potential mutagenic activity is a critical step in the assessment of the safety of both new and pre-existing chemical types. In Genetic Toxicology: Principles and Methods, expert contributors help to satisfy the demand for education in this tremendously important area of study. The volume covers three basic areas: the scientific basis of the discipline, the methodologies of the main test assays, and the application of the methods, all aimed primarily at scientists in the safety departments of the industries working with both natural and synthetic chemicals. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Intuitive and cutting-edge, Genetic Toxicology: Principles and Methods provides crucial support to both laboratory workers in providing quality information on the appropriate application of techniques and to study directors in their assay selection and protocol design in this vital field.
Author: Mahin D. Maines
Publisher: Current Protocols
ISBN: 9780471241065
Category : Science
Languages : en
Pages : 700
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Book Description
Provides the latest models and methods from molecular biology, cell biology, biochemistry, and genetics, plus sophisticated toxicological procedures from leading laboratories. Also offers guidelines for evaluating the effects of substances on human physiology and metabolism and reference information, including stock solutions and equipment and commonly used techniques.
Author: George P. Daston
Publisher: CRC Press
ISBN: 1439810664
Category : Medical
Languages : en
Pages : 336
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Book Description
This new manual provides a convenient source of experimental procedures, including the most modern and frequently used molecular and cellular techniques. Experimental protocols have been carefully selected by developmental toxicologists for developmental toxicologists. The most important new trends, such as evaluation of the safety of therapeutic antisense oligonucleotides, studies of the role of cell death in abnormal development, and the identification of sparingly expressed developmental control genes are featured. This is the perfect manual for scientists trained in classical developmental toxicology who want to add molecular and cellular methods to their research.
Author: Emilio Benfenati
Publisher:
ISBN: 9781071619605
Category : Computer simulation
Languages : en
Pages : 680
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Book Description
This fully updated book explores all-new and revised protocols involving the use of in silico models, particularly with regard to pharmaceuticals. Divided into five sections, the volume covers the modeling of pharmaceuticals in the body, toxicity data for modeling purposes, in silico models for multiple endpoints, a number of platforms for evaluating pharmaceuticals, as well as an exploration of challenges, both scientific and sociological. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and implementation advice necessary for successful results. Authoritative and comprehensive, In Silico Methods for Predicting Drug Toxicity, Second Edition aims to guide the reader through the correct procedures needed to harness in silico models, a field which now touches a wide variety of research specialties.
Author:
Publisher:
ISBN: 9780471503385
Category : Molecular biology
Languages : en
Pages :
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Book Description
