Molecular Techniques in Taxonomy PDF Download
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Author: Godfrey M. Hewitt
Publisher: Springer Science & Business Media
ISBN: 3642839622
Category : Science
Languages : en
Pages : 404
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Book Description
Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing.
Author: Godfrey M. Hewitt
Publisher: Springer Science & Business Media
ISBN: 3642839622
Category : Science
Languages : en
Pages : 404
Get Book Here
Book Description
Taxonomy is fundamental to understanding the variety of life forms, and exciting expansions in molecular biology are re- volutionising the obtained data. This volume reviews the ma- jor molecular biological techniques that are applied in ta- xonomy. The chapters are arranged in three main sections:1) Overviews of important topics in molecular taxonomy; 2) Case studies of the successful application of molecular methods to taxonomic and evolutionary questions; 3) Protocols for a range of generally applicable methods. The described techni- ques include DNA-DNA hybridization, DNA fingerprinting, RFLP analysis, and PCR sequencing.
Author: Pascale Besse
Publisher: Humana
ISBN: 9781627037662
Category : Science
Languages : en
Pages : 0
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Book Description
Plant taxonomy is an ancient discipline facing new challenges with the current availability of a vast array of molecular approaches which allow reliable genealogy-based classifications. Although the primary focus of plant taxonomy is on the delimitation of species, molecular approaches also provide a better understanding of evolutionary processes, a particularly important issue for some taxonomic complex groups. Molecular Plant Taxonomy: Methods and Protocols describes laboratory protocols based on the use of nucleic acids and chromosomes for plant taxonomy, as well as guidelines for phylogenetic analysis of molecular data. Experts in the field also contribute review and application chapters that will encourage the reader to develop an integrative taxonomy approach, combining nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Molecular Plant Taxonomy: Methods and Protocols seeks to provide conceptual as well as technical guidelines to plant taxonomists and geneticists.
Author: Pascale Besse
Publisher: Humana
ISBN: 9781071609996
Category : Science
Languages : en
Pages : 400
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Book Description
This fully updated edition explores conceptual as well as technical guidelines for plant taxonomists and geneticists, such as the increasing use of next-generation sequencing (NGS) technologies for numerous applications in plant taxonomy. The volume provides molecular approaches to be used within an “integrative taxonomy” framework, combining a range of nucleic acid and cytogenetic data together with other crucial information (taxonomy, morphology, anatomy, ecology, reproductive biology, biogeography, paleobotany, etc.), which will help not only to best circumvent species delimitation but also to resolve the evolutionary processes in play. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Molecular Plant Taxonomy: Methods and Protocols, Second Edition is an ideal guide for researchers seeking a better understanding of evolutionary processes, at species and population level, through molecular techniques.
Author: Pamela S. Soltis
Publisher: Springer Science & Business Media
ISBN: 1461532760
Category : Science
Languages : en
Pages : 446
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Book Description
The application of molecular techniques is rapidly transforming the study of plant systematics. The precision they offer enables researchers to classify plants that have not been subject to rigorous classification before and thus allows them to obtain a clearer picture of evolutionary relationships. Plant Molecular Systematics is arranged both conceptually and phylogenetically to accommodate the interests not only of general systematists, but also those of people interested in a particular plant family. The first part discusses molecular sequencing; the second reviews restriction site analysis and the sequencing of mitochondrial DNA. A third section details the analysis of ribosomal DNA and chloroplast DNA. The following section introduces model studies involving well-studied families such as the Onagraceae, Compositae and Leguminosae. The book concludes with a section addressing theoretical topics such as data analysis and the question of morphological vs. molecular data.
Author: Donald L.J. Quicke
Publisher: Springer Science & Business Media
ISBN: 9401121346
Category : Science
Languages : en
Pages : 323
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Book Description
Taxonomy is an ever-changing, controversial and exCitmg field of biology. It has not remained motionless since the days of its founding fathers in the last century, but, just as with other fields of endeavour, it continues to advance in leaps and bounds, both in procedure and in philosophy. These changes are not only of interest to other taxonomists, but have far reaching implications for much of the rest of biology, and they have the potential to reshape a great deal of current biological thought, because taxonomy underpins much of biological methodology. It is not only important that an ethologist. physiologist. biochemist or ecologist can obtain information about the identities of the species which they are investigating; biology is also uniquely dependent on the comparative method and on the need to generalize. Both of these necessitate knowledge of the evolutionary relationships between organisms. and it is the science of taxonomy that can develop testable phylogenetic hypotheses and ultimately provide the best estimates of evolutionary history and relationships.
Author:
Publisher:
ISBN: 9780815332183
Category : Cells
Languages : en
Pages : 0
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Book Description
Author: Suan Choo Cheah
Publisher:
ISBN:
Category :
Languages : en
Pages : 13
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Book Description
Author: Heather B. Miller
Publisher: Academic Press
ISBN: 0123855454
Category : Science
Languages : en
Pages : 227
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Book Description
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author: F.G. Priest
Publisher: Springer Science & Business Media
ISBN: 1461518695
Category : Medical
Languages : en
Pages : 329
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Book Description
Bacterial taxonomy as a specialized discipline is practised by a minority but the applications of taxonomy are important to most, if not all microbiologists. It is the implementation of taxonomic ideas and practises which gives rise to identification and typing systems, procedures for the analysis and characterization of biodiversity, hypotheses about the evolution of micro-organisms, and improved procedures for the isolation and implementation of bacteria in biotechnological processes. Without taxonomic theory providing a sound basis to these many facets of microbiology there would be severe problems faced by many scientists working with micro-organisms. Taxonomy comprises three sequential but independent processes; classification, nomenclature and identification. The first two stages are the prime concern of the specialist taxonomist but the third stage should result in identification schemes of value to all microbiologists. As the classification and identification of micro-organisms improves, largely due to the introduction of new technologies, so does its contribution to the subject as a whole. It therefore seemed timely to hold a conference in the autumn of 1993 devoted to microbial identification. Such a topic could not be addressed without some reference to the enabling discipline of classification, but the principal aims were to assess improvements in identification and typing and how these were benefiting microbiological topics ranging from ecological and biotechnological studies of extremophilic bacteria to the use of pyrolysis mass spectrometry in epidemiology. The meeting, which was held in Granada, Spain, was supported by FEMS (FEMS Symposium No.
Author: B. Sneh
Publisher: Springer Science & Business Media
ISBN: 9401729018
Category : Science
Languages : en
Pages : 561
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Book Description
Rhizoctonia Species: Taxonomy, Molecular Biology, Ecology, Pathology and Control, written by the world's most reputable experts in their respective fields of Rhizoctonia research, summarizes years of research in the various aspects of the ubiquitous complex group of soil-borne fungi belonging to the anamorph genus Rhizoctonia. Species of Rhizoctonia worldwide cause economically important diseases on most of the world's important plants such as cereals, potato, cotton, sugarbeet, vegetables, ornamentals and trees in nurseries. The subject reviews covered in the book include classic as well as modern approaches to Rhizoctonia research in: Taxonomy and Evolution, Genetics and Pathogenicity, Plant-Rhizoctonia Interactions, Ecology, Population and Disease Dynamics, Disease Occurrence and Management in Various Crops, Cultural Control, Biological Control, Germplasm for Resistance, Chemical and Integrated Control Strategies. It aims to be the standard reference source book on Rhizoctonia for the next decade or more, just as Parmeter et al. (1970) has been in the past. It will be an important publication for Rhizoctonia investigators, plant pathologists, students, extension specialists, crop producers and companies dealing with plant disease control.
