Mitochondrial Involvement and Plasticity in Muscle Aging and Age-related Disease PDF Download

Author: Yana Konokhova
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Languages : en
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Book Description
"Skeletal muscle plasticity is highly dependent on mitochondrial function. Mitochondria regulate critical intracellular functions, including energy production in the form of oxidative phosphorylation and intrinsic pathways of apoptosis. As such, mitochondrial impairments are implicated in adverse muscle impact seen in numerous age-associated diseases as well as healthy aging. For example, low mitochondrial content and impaired oxidative capacity are well-characterized features of locomotor muscle of patients with Chronic Obstructive Pulmonary Disease (COPD). High oxidative stress and physical inactivity are typical of COPD pathophysiology and have been proposed to contribute to low muscle oxidative capacity. At the single fiber level, oxidative capacity can be compromised from insufficient quantities of functional mitochondrial DNA (mtDNA). Our investigations revealed that the high oxidative stress milieu of COPD muscle corresponded to increased incidence of mtDNA mutations and a significantly higher prevalence of fibers lacking cytochrome oxidase (COX) activity secondary to high mtDNA mutation load, compared to age-matched healthy controls. Importantly, in healthy controls, these COX-deficient fibers, though infrequent in prevalence, exhibited a focal increase in mitochondrial biogenesis (including upregulation of mitochondrial biogenesis signals and resulting increases in mtDNA content) to try to reverse the cellular energetic insufficiency. However in COPD muscle, these fibers were much more abundant and the focal compensatory response was absent. Furthermore, our second investigation demonstrated that an endurance-training program (a potent stimulus to upregulate mitochondrial biogenesis) did not ameliorate the abnormal signaling in COX-deficient fibers and did not restore the blunted mtDNA replicative response in such fibers compared to untrained, but healthy controls. Collectively, these results point towards an impairment in mitochondrial biogenesis in COPD muscle that goes beyond that which can be ascribed to the very low physical activity seen in patients. Mitochondria have also been implicated in age-related muscle loss. As such, clinically significant loss of muscle mass and function occur with aging even in the absence of chronic disease. Dysregulated mitochondrial function and increased apoptosis have been previously implicated, particularly in fast-twitch muscle. As such, the third project investigated very old muscles with divergent fiber types and revealed that after accounting for age-related shifting in myosin heavy chain composition, mitochondria in atrophying muscles are more susceptible to initiating apoptosis irrespective of muscle fiber type composition. Furthermore, there was a marked increase in the nuclear translocation of the mitochondrial-derived pro-apoptotic factor, apoptosis inducing factor (AIF), in muscles undergoing atrophy irrespective of fiber type composition. Notably, there was no sensitization to apoptosis or change in myonuclear AIF in a muscle that did not atrophy in very advanced age (adductor longus muscle). As such, these results are consistent with the notion that mitochondrial-mediated apoptosis secondary to sensitization of the mitochondria to permeability transition is involved in the atrophy of skeletal muscle with aging and that this process is independent of the muscle fiber type composition. In summary, this thesis presents evidence of an impairment in mitochondrial biogenesis in COPD locomotor muscle secondary to impaired mtDNA replication that is not restored by exercise training. Furthermore, my results provide the first evidence that a sensitization to permeability transition and translocation of mitochondrial-derived pro-apoptotic factors occurs exclusively in atrophying fast and slow twitch muscles with aging, identifying a process that may help explain the atrophy of aging skeletal muscle irrespective of muscle fiber type composition." --