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Author: Volker Neuhoff
Publisher: Springer Science & Business Media
ISBN: 3642807615
Category : Science
Languages : en
Pages : 439
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Book Description
This book is based on practical experience and is therefore written as a prac tical manual. The fore-runners of the book were the manuals of the first and second EMBO-Courses on "Micromethods in Molecular Biology" which were held in G6ttingen in the spring of 1970 and the autumn of 1971. This book may serve as a manual not only for the participants of the third EMBO-Course to be held in G6ttingen in autumn 1973, but also for all experimenters who are interested in using micromethods. It must be emphasized from the outset that this book is conceived as a "cook book" and not as a monograph which attempts to revue the literature on micromethods critically. The methods described here in detail are performed routinely in the authors' laboratories and include all the practical details necessary for the successful appli cation of the micromethods. There are many other sensitive and excellent micro methods which are not included in this book, because the authors feel that in a "cook book" only methods for which they have personal experience and profi ciency should be described. Some readers may feel that the title promises more than the present contents of this book; however, if sufficient interest is shown in this volume, it may be possible to remedy such deficiencies in future editions.
Author: Volker Neuhoff
Publisher: Springer Science & Business Media
ISBN: 3642807615
Category : Science
Languages : en
Pages : 439
Get Book Here
Book Description
This book is based on practical experience and is therefore written as a prac tical manual. The fore-runners of the book were the manuals of the first and second EMBO-Courses on "Micromethods in Molecular Biology" which were held in G6ttingen in the spring of 1970 and the autumn of 1971. This book may serve as a manual not only for the participants of the third EMBO-Course to be held in G6ttingen in autumn 1973, but also for all experimenters who are interested in using micromethods. It must be emphasized from the outset that this book is conceived as a "cook book" and not as a monograph which attempts to revue the literature on micromethods critically. The methods described here in detail are performed routinely in the authors' laboratories and include all the practical details necessary for the successful appli cation of the micromethods. There are many other sensitive and excellent micro methods which are not included in this book, because the authors feel that in a "cook book" only methods for which they have personal experience and profi ciency should be described. Some readers may feel that the title promises more than the present contents of this book; however, if sufficient interest is shown in this volume, it may be possible to remedy such deficiencies in future editions.
Author: Georg Keleti
Publisher: Van Nostrand Reinhold Company
ISBN:
Category : Science
Languages : en
Pages : 192
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Book Description
"This handbook has 106 procedures each specifically detailing a biochemical or microbiological method of preparation or analysis. They are written in a concise, step-by-step form ... Reagents are specified ... Exact methods of preparation are delineated. Illustrations are provided where required to clarify a method." Pref. Indexed.
Author: Harald Tschesche
Publisher: Walter de Gruyter
ISBN: 3110252368
Category : Science
Languages : en
Pages : 379
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Book Description
This book presents a survey of recent developments in protein biochemistry. Top researchers in the field of protein biochemistry describe modern methods to address the challenges of protein purification by three-phase partitioning, and their folding and degradation by the functions of chaperones. The significance of peptide purity for fibril formation is addressed as well as the use of target oriented peptide arrays in palliative approaches in mucoviszidose. The design and application of protein epitope mimetics just as the structural resolving of the misfolding of various mutant proteins in serpinopathies enlarge our tools in resolving pathophysiological imbalances.
Author: Harald Tschesche
Publisher: Walter de Gruyter GmbH & Co KG
ISBN: 311239304X
Category : Science
Languages : en
Pages : 448
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Book Description
No detailed description available for "Review Articles, including those from an International Conference held in Bielefeld, F. R. of Germany, June 1-2, 1984".
Author: Jose V. Castell
Publisher: Elsevier
ISBN: 0080534600
Category : Medical
Languages : en
Pages : 483
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Book Description
In Vitro Methods in Pharmaceutical Research provides a comprehensive guide to laboratory techniques for evaluating in vitro organ toxicity using cellular models. Step-by-step practical tips on how to perform and interpret assays for drug metabolism and toxicity assessment are provided, along with a comparison of different techniques available. It is a welcome addition to the literature at a time when interest is growing in cellular in vitro models for toxicology and pharmacology studies. - Meets the continuing demand for information in this field - Compares In Vitro techniques with other methods - Describes cell-culture methods used to investigate toxicity in cells derived from different organs - Includes contributions by leading experts in the field
Author: National Library of Medicine (U.S.)
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages :
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Book Description
First multi-year cumulation covers six years: 1965-70.
Author: Ivan Lefkovits
Publisher: Academic Press
ISBN: 148326999X
Category : Science
Languages : en
Pages : 492
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Book Description
Immunological Methods a compendium of basic research techniques being used in one of the largest immunology research institutes, the Basel Institute for Immunology, with particular emphasis given to new methodology. The procedures have been described by individuals judged to be highly expert in their specialties. In many instances the methods developed or adapted to unique uses by the contributors have not previously been described in detail. The book contains 34 chapters covering techniques for detection, isolation, and purification of antibodies (including dansylation, two-dimensional chromatography, isoelectric focusing, polyacrylamide gel electrophoresis, and isotachophoresis); measurement of equilibrium constants (equilibrium dialysis, filtration, and sedimentation); and isotope and fluorescent labeling and detection of cell-surface components. Techniques such as isotope laboratory maintenance; chemical modification of proteins, haptens, and solid supports, and haptenation of viable biological carriers; production of antisera against allotypes and histocompatibility antigens and production of antibody with clonai dominance; histocompatibility and MLR testing; and cell separation by haptenated gels and by velocity sedimentation of rosette-forming cells are also discussed. Other chapters cover detection of antibody-secreting and alloantigen-binding cells; immune responses in vitro and their analysis by limiting dilution; production of T-cell factors; hybridoma production by cell fusion; maintenance of cell lines and cloning in semisolid media; and the mathematical analysis of immunological data.
Author: Abel Lajtha
Publisher: Springer Science & Business Media
ISBN: 1468442082
Category : Science
Languages : en
Pages : 486
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Book Description
The second volume of the Handbook does not parallel any volume of the first edition; it is one more sign, or reflection, of the expansion of the field. By emphasizing the experimental approach, it illustrates the tools that have re cently become available for investigating the nervous system. Also, perhaps even more than other volumes, it illustrates the multidisciplinary nature of the field, requiring multidisciplinary methodology. It is now recognized that the availability of methodology is often the rate-limiting determinant of studies and that improvements or innovations in instrumentation can open up new avenues. A new improved method, although opening up new possibilities and being crucial to making advances, is only a tool whose use will determine its use fulness. If we do not recognize its possibilities, its use will be limited; if we do not recognize its limitations, it will mislead us. It is the possibilities and limitations and the results obtained that are illustrated here.
Author: G. A. Meek
Publisher: Cambridge University Press
ISBN: 9780521214049
Category : Science
Languages : en
Pages : 292
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Book Description
Author: Neville Marks
Publisher: Springer Science & Business Media
ISBN: 1461344581
Category : Medical
Languages : en
Pages : 476
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Book Description
With the continued rapid expansion of neurochemical research, there has been no shortage of new developments in methodology for this third volume of Research Methods in Neurochemistry. As in previous volumes we have again tried to provide some balance in the subjects represented. The wisdom of this policy may be questioned since it can lead to delay in publica tion, but there are many approaches to the chemical study of the nervous system and a methods book needs to stand on its own as well as be part of a series. In one respect, however, the present volume departs from this policy, in that we have included two chapters on micromethods for analyzing amines and amino acids, both giving special emphasis to dansylation techniques. These chapters are complementary and we feel justified in publishing them in one volume in view of the importance of such micromethods for the study of neural systems. At the other end of the scale, particular attention may be drawn to the chapter by D. D. Gilboe and colleagues describing their remarkable procedures for studying metabolism in the isolated canine brain. We were fortunate also in persuading S. S. Oja to extend the general prin ciples of transport systems he described in Volume 2 to amino acids in brain slices. In addition, there are the usual chapters on components of neural tissues, which once again we have found convenient to divide into enzymes, macromolecules, and other constituents.
