Micromanipulation of Biological Particles with Optical Tweezers PDF Download
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Author: Sonia Bayoudh
Publisher:
ISBN:
Category : Particles
Languages : en
Pages : 125
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Book Description
Author: Sonia Bayoudh
Publisher:
ISBN:
Category : Particles
Languages : en
Pages : 125
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Book Description
Author: Karl Otto Greulich
Publisher: Springer Science & Business Media
ISBN: 1461241103
Category : Science
Languages : en
Pages : 308
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Book Description
There are probably few people who do not dream of the good old times, when do ing science often meant fascination, excitement, even adventure. In our time, do ing science involves often technology and, perhaps, even business. But there are still niches where curiosity and fascination have their place. The subject of this book, technological as its title may sound, is one of the fortunate examples. It will report on lasers generating the coldest places in the Universe, and on table top laser microtools which can produce a heat "inferno" as it prevails in the interior of the Sun, or simulate, for specific plant cells, microgravity of the space around our plan et Earth. There will be some real surprises for the reader. The applications range from basic studies of the driving forces of cell division (and thus life) via genetic modification of cells (for example, for plant breeding) to medical applications such as blood cell analysis and finally in vitro fertilization. What are these instruments: laser microbeams and optical tweezers? Both are lasers coupled with a fluorescence microscope. The laser microbeam uses a pulsed ultraviolet laser. Light is focused, as well as possible, in space and time, in order to obtain extremely high light intensities - high enough to generate, for a very short instant, extremely hot spots which can be used to cut, fuse or perforate biological material.
Author: Miles J. Padgett
Publisher: CRC Press
ISBN: 1420074148
Category : Science
Languages : en
Pages : 510
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Book Description
The technical development of optical tweezers, along with their application in the biological and physical sciences, has progressed significantly since the demonstration of an optical trap for micron-sized particles based on a single, tightly focused laser beam was first reported more than twenty years ago. Bringing together many landmark papers on
Author: Arne Gennerich
Publisher: Springer Nature
ISBN: 1071622293
Category : Science
Languages : en
Pages : 753
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Book Description
This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Optical Tweezers: Methods and Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.
Author: Arthur Ashkin
Publisher: World Scientific
ISBN: 9814494348
Category : Science
Languages : en
Pages : 941
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Book Description
This important volume contains selected papers and extensive commentaries on laser trapping and manipulation of neutral particles using radiation pressure forces. Such techniques apply to a variety of small particles, such as atoms, molecules, macroscopic dielectric particles, living cells, and organelles within cells. These optical methods have had a revolutionary impact on the fields of atomic and molecular physics, biophysics, and many aspects of nanotechnology.In atomic physics, the trapping and cooling of atoms down to nanokelvins and even picokelvin temperatures are possible. These are the lowest temperatures in the universe. This made possible the first demonstration of Bose-Einstein condensation of atomic and molecular vapors. Some of the applications are high precision atomic clocks, gyroscopes, the measurement of gravity, cryptology, atomic computers, cavity quantum electrodynamics and coherent atom lasers.A major application in biophysics is the study of the mechanical properties of the many types of motor molecules, mechanoenzymes, and other macromolecules responsible for the motion of organelles within cells and the locomotion of entire cells. Unique in vitro and in vivo assays study the driving forces, stepping motion, kinetics, and efficiency of these motors as they move along the cell's cytoskeleton. Positional and temporal resolutions have been achieved, making possible the study of RNA and DNA polymerases, as they undergo their various copying, backtracking, and error correcting functions on a single base pair basis.Many applications in nanotechnology involve particle and cell sorting, particle rotation, microfabrication of simple machines, microfluidics, and other micrometer devices. The number of applications continues to grow at a rapid rate.The author is the discoverer of optical trapping and optical tweezers. With his colleagues, he first demonstrated optical levitation, the trapping of atoms, and tweezer trapping and manipulation of living cells and biological particles.This is the only review volume covering the many fields of optical trapping and manipulation. The intention is to provide a selective guide to the literature and to teach how optical traps really work.
Author:
Publisher: Academic Press
ISBN: 0080859534
Category : Science
Languages : en
Pages : 241
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Book Description
Volume 55 in Methods in Cell Biology is a concise laboratory book that emphasizes the methods and technologies needed to use single polarized laser light source that functions simultaneously as an optical trap and a dual-beam interferometer. * * Provides a practical laboratory guide for methods and technologies used with laser tweezers* Includes comprehensive and easy-to-follow protocols
Author: Marinela Gjoni Boillat
Publisher:
ISBN:
Category :
Languages : en
Pages : 121
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Book Description
Author: Arthur Ashkin
Publisher: World Scientific Publishing Company Incorporated
ISBN: 9789810240585
Category : Science
Languages : en
Pages : 915
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Book Description
This important volume contains selected papers and commentaries on laser trapping and manipulation of neutral particles using radiation pressure forces. These revolutionary optical techniques apply to atoms, molecules, dielectric particles, living cells and organelles within cells. They have made possible the cooling of atoms to the lowest temperature in the universe, the first demonstration of Bose-Einstein condensation of atomic vapors, the measurement of the driving forces of individual molecular motors, and the observation of their stepping motion. Only simple geometrical optics and semiclassical physics are used to explain the light forces and traps. The author is the discoverer of optical trapping and optical tweezers. With his colleagues he first demonstrated optical levitation, trapping of atoms, and tweezer trapping and manipulation of living cells and biological particles. This is the only review covering the entire scope of optical manipulation. The intention is to provide a selective guide to the literature and teach how optical traps really work.
Author: Zhenjiang Ni
Publisher: John Wiley & Sons
ISBN: 1119007941
Category : Technology & Engineering
Languages : en
Pages : 205
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Book Description
The authors of this book provide the first review of haptic optical tweezers, a new technique which brings together force feedback teleoperation and optical tweezers. This technique allows users to explore the microworld by sensing and exerting piconewton-scale forces with trapped microspheres. The design of optical tweezers for high-quality haptic feedback is challenging, given the requirements for very high sensitivity and dynamic stability. The concept, design process and specification of optical tweezers reviewed throughout this book focus on those intended for haptic teleoperation. The authors provide two new specific designs as well as the current state of the art. Furthermore, the remaining important issues are identified for further developments. Haptic optical tweezers will soon become an invaluable tool for force feedback micromanipulation of biological samples and nano- and micro-assembly parts.
Author: Peifeng Jing
Publisher:
ISBN:
Category :
Languages : en
Pages : 104
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Book Description
Optical Tweezers is a valuable and versatile tool in the non-invasive manipulation of micro- and nano-sized particles for biology studies and nanotechnologies. In this dissertation, we propose and demonstrate a two-dimensional photonic crystal enhanced optical tweezers for particles and biological cells with higher trapping efficiency and less photodamage or heat generation. The capabilities of this enhanced optical tweezers include trapping, transporting and patterning latex beads and cells. The trapping efficiency is significantly improved based on the higher gradient of laser intensity distribution. This technology relaxes the requirement on input optical power without additional heat generation, which extends the viability of living cells in the trap. Moreover, we developed a flexible platform using parylene-C integrated with photonic crystal enhanced optical tweezers. This system is utilized for colonizing patterned cells with optimized oxygen plasma treated parylene-C film, as well as measuring the cellular repulsive force between a pair of single cells.
