Microfluidic Device for Continuous Deformability Based Separation of Circulating Tumor Cells PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Microfluidic Device for Continuous Deformability Based Separation of Circulating Tumor Cells PDF full book. Access full book title Microfluidic Device for Continuous Deformability Based Separation of Circulating Tumor Cells by Chao Jin. Download full books in PDF and EPUB format.
Author: Chao Jin
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Author: Chao Jin
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
Author: Hashem Mohammad Abul
Publisher:
ISBN:
Category : Cancer
Languages : en
Pages :
Get Book Here
Book Description
Circulating Tumor Cells (CTCs) are potential indicators of cancer. Detection of CTCs is important for diagnosing cancer at an early stage and predicting the effectiveness of cancer treatment. Recent progress in the development of microfluidic chips has inaugurated a new possibility for designing diagnostic devices for early cancer detection. Among various devices, deformation-based CTC microchips have shown a strong promise for CTC detection due to its simplicity and low cost. This type of devices involves a process where CTCs are trapped while allowing more deformable blood cells to squeeze through the filtration geometry at the specified operating pressure. Fundamental understanding of CTC passing event through a micro-filtering channel seems to be a promising direction in studying these microdevicessince it helps optimize the microfilter design for achieving high isolation purity and capture efficiency. Along with the experimental studies, numerical simulation emerges as a powerful tool to predict the behavior of a cell inside a microfilter, and may deliver important insights to optimize the processes by saving time and cost. First, the CTC squeezing process through a microfluidic filtering channel is studied by modeling the CTC as a simple liquid droplet. Cell modeling employed both Newtonian and non-Newtonian approaches to simplify the model and investigating different biophysical properties. Detailed microscopic multiphase flow characteristics regarding the filtering process are discussed including the pressure signatures, flow details, and cell deformation. Next, we employed a compound droplet model consisting of an outer cell membrane, cytoplasm and the nucleus to study the flow dynamics more realistically. The effects of different parameters such as the nuclear to cytoplasmic size ratio (N/C), operating flow rate and viscosity of the cell has been investigated. We studied critical pressure for the CTC at different flow rates as it plays a crucial role in the device operation in ensuring a successful passing event. Our study provides an insight into the cell squeezing process and its characteristics, which can guide in the design and optimization of next-generation deformation-based CTC microfilters.
Author: Takasi Nisisako
Publisher: MDPI
ISBN: 3039366947
Category : Technology & Engineering
Languages : en
Pages : 230
Get Book Here
Book Description
Microfluidic platforms are increasingly being used for separating a wide variety of particles based on their physical and chemical properties. In the past two decades, many practical applications have been found in chemical and biological sciences, including single cell analysis, clinical diagnostics, regenerative medicine, nanomaterials synthesis, environmental monitoring, etc. In this Special Issue, we invited contributions to report state-of-the art developments in the fields of micro- and nanofluidic separation, fractionation, sorting, and purification of all classes of particles, including, but not limited to, active devices using electric, magnetic, optical, and acoustic forces; passive devices using geometries and hydrodynamic effects at the micro/nanoscale; confined and open platforms; label-based and label-free technology; and separation of bioparticles (including blood cells), circulating tumor cells, live/dead cells, exosomes, DNA, and non-bioparticles, including polymeric or inorganic micro- and nanoparticles, droplets, bubbles, etc. Practical devices that demonstrate capabilities to solve real-world problems were of particular interest.
Author: Xiaolong Zhang
Publisher:
ISBN:
Category : Mechanical engineering
Languages : en
Pages :
Get Book Here
Book Description
Author: Jose L. Garcia-Cordero
Publisher: Springer Nature
ISBN: 107163271X
Category : Medical
Languages : en
Pages : 327
Get Book Here
Book Description
This detailed volume explores recent developments in microfluidics technologies for cancer diagnosis and monitoring. The book is divided into two sections that delve into techniques for liquid biopsy for cancer diagnosis and platforms for precision oncology or personalized medicine in order to create effective patient avatars for testing anti-cancer drugs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Microfluidic Systems for Cancer Diagnosis serves as an ideal guide that will be helpful to either replicate the construction of microfluidic devices specifically developed for cancer diagnosis or to catalyze development of new and better cancer diagnostic devices.
Author: Nika Nikbakht
Publisher:
ISBN: 9781321854633
Category :
Languages : en
Pages : 67
Get Book Here
Book Description
Circulating tumor cells (CTCs) are cells that shed into the vasculature from a primary tumor and circulate in the bloodstream. CTCs can be used to elucidate the molecular characterization of the tumor cells and to gauge the efficiency of therapeutic treatment in metastatic carcinoma patients. They can also be used to determine the primary site of the tumor in areas where the tumor is undetectable with traditional oncological imaging. The detection of CTCs has a substantial value for prognostic and therapeutic implications, but they are not easily detected because of their low cell count. Because microfluidic devices are useful for cell detection and diagnosis, can be easily obtained, and are less invasive than tissue biopsies, we have developed a microfluidic platform to capture CTCs using multiple capture targets to achieve a higher cell capture. We can selectively isolate the cancer cells using specific antibodies to the antigen capture target on the surface of malignant cells. The capture efficiency was evaluated by the flow rate, cell count, and antibody immobilization. Cancer cell lines that were known to have high expression for targeted ligands, specifically HER2, EGFR, EpCAM, and MUC-1, were tested with antibodies specific to these ligands. We obtained capture efficiency with these different capture targets on a single channel. This allowed us to develop a device with four parallel capture channels to run in series with the anticipation of achieving higher cell capture.
Author: Mohammed Khan
Publisher:
ISBN:
Category : Dielectrophoresis
Languages : en
Pages :
Get Book Here
Book Description
Separation of pure population of cells from complex and heterogeneous cell mixtures has immense application on therapeutics and diagnostics of diseases. Chinese hamster ovary (CHO) cell in its viable form is the most widely used mammalian cell line for commercial production of therapeutic protein. On the other hand, Circulating tumor cells (CTCs) are proven elements to have significant prognostic, diagnostic, and clinical values in early-stage cancer detection. Thus, separation of nonviable CHO cells from suspending medium is critical in biopharmaceutical sectors and isolation of CTCs from peripheral blood for proper drug innovation in medical sectors. Passive microfluidic techniques, which can use the geometry to utilize intrinsic fluidic forces to separate different sized cells, are getting attention as a high-throughput microchip technique. Despite extensive performance to separate different size cells, this technique suffers when similarity of size between cells of specific types of CTCs and WBCs or viable and non-viable CHO cells are present. On the other hand, an active technique, i.e., dielectrophoresis, lacks the desired high throughput. Here, two different hybrid microfluidic techniques, one with Deterministic Lateral Displacement (DLD) and another with inertial microfluidics, are tested with CTC and CHO. First, we demonstrated that a DLD device could be combined with a frequency-based AC electric field to perform high-resolution continuous separation of nonviable CHO cells from the viable cells. This coupled DLD-DEP device's behavior is further investigated by employing numerical simulation to check the effect of geometrical parameters of the DLD arrays, velocities of the flow field, and required applied voltages. In the following work, we proposed a hybrid technique that combines inertia microfluidics and dielectrophoresis in order to separate CTCs from overlapping size WBCs through the use of a sheath flow. The cell trajectories along with fluid fields are modeled to investigate the effects of different applied electrical voltages and Reynold numbers on separation characteristics. This technique could be exploited to design a microchip for continuous separation for any cell beads, controlled simply by adjusting the external field frequency.
Author: Shrutilaya Karunanidhi
Publisher:
ISBN: 9781303229893
Category :
Languages : en
Pages : 68
Get Book Here
Book Description
Cells that break off from the primary tumor, known as circulating tumor cells are often the cause of metastasis in cancer patients. Their isolation and characterization is pivotal for various reasons such as molecular characterization of the tumor cells, treatment monitoring, and also to determine the primary site of the tumor in cases where the tumor itself is undetectable, however, this task remains a major challenge as these cells are extremely rare in the blood vessels. Numerous research groups have presented microfluidic approaches that are capable of isolation and capture of rare cells. Recently, inertial microfluidics is one such approach that has gained much attention for this application. In these systems, various hydrodynamic forces generated in the microchannels are used for size-based focusing of particles into distinct streams. Based on this concept, we developed fourteen different microfluidic devices using poly(dimethylsiloxane) (PDMS) polymer. Each device had a typical set of nine parameters like channel width, location of branches, position of first branch and number of loops. The devices were tested with a binary mixture of polystyrene beads as the sample solution at various flow rates and concentration ratios. Several hypotheses were tested and inferences were drawn to determine the most efficient design in terms of the capture efficiency and isolation efficiency of the device. The final device design achieved an isolation and capture efficiency of>90%, thereby, making it a better alternate for cancer screening.
Author: Manabu Tokeshi
Publisher: Springer
ISBN: 9811362297
Category : Science
Languages : en
Pages : 387
Get Book Here
Book Description
This book focuses on state-of-the-art microfluidic research in medical and biological applications. The top-level researchers in this research field explain carefully and clearly what can be done by using microfluidic devices. Beginners in the field —undergraduates, engineers, biologists, medical researchers—will easily learn to understand microfluidic-based medical and biological applications. Because a wide range of topics is summarized here, it also helps experts to learn more about fields outside their own specialties. The book covers many interesting subjects, including cell separation, protein crystallization, single-cell analysis, cell diagnosis, point-of-care testing, immunoassay, embyos/worms on a chip and organ-on-a-chip. Readers will be convinced that microfluidic devices have great potential for medical and biological applications.
Author: Yiqiu Xia
Publisher:
ISBN:
Category :
Languages : en
Pages :
Get Book Here
Book Description
As a major healthcare concern, highly pathogenic viral infection can spread globally with modern transportation. Viral infectious diseases have caused some of the deadliest pandemics and heavily damaged global economy in recorded human history. As we prepare for the next major emerging viral infectious disease outbreak, there is an urgent need for the development of new techniques that can rapidly detect viruses and perform surveillance of viral infectious diseases at any location. On the other and, cancer is a major disease in human society nowadays, leading to the second most deaths worldwide. Circulating tumor cell (CTC) has been established as a liquid biopsy marker, however, there are demands of fast and accurate CTC detection. Microfluidics has the advantages of high throughput, high sensitivity, accurate flow rate control and low cost, allowing it well suited for virus and cancer diagnosis. Besides, the geometry of microfluidics allows precisely controlling of the physical, chemical, biological, and physiological environment at the cellular level or even at the molecular level for fundamental studies of cancers.My major works can be classified into two categories, microfluidic devices for virus diagnosis and microfluidic platforms towards cancer diagnosis. For the virus diagnosis, one microfluidic device for size-based virus isolation and another one for immunoaffinity-based virus detection are developed, respectively. In the first device, inter-wire size-tunable porous silicon nanowire forest is embedded inside the microfluidic channel to trap avian influenza viruses based on their size and then release trapped viruses by nanowire degradation. About 50% of virus can be captured and 60% of trapped virus can be released for culture and further analysis. In the second device, immunoassay is employed inside the channels to capture and detect virus in only ~1.5 hours. Colorimetric reaction with gold nanoparticles and silver enhancer allow detection with naked eyes with about one order of magnitude better than conventional fluorescent enzyme-linked immunosorbent assay (ELISA). Simply by introducing an optical detection scheme with a smartphone detection system, the sensitivity can be 30 times better than conventional fluorescent ELISA. Two microfluidic platforms were developed toward cancer diagnosis. The first microfluidic platform aims to study the process of CTC size-based microfiltration and cancer cell translocating through micro constrictions by mimicking the microfiltration process and in vivo micro-constrictions inside a microfluidic device. It is found that the deformability and size of nucleus instead of the whole cell dominate cellular translocation through micro constrictions under the normal physiological pressure range used by CTC microfiltration. The result is consistent with the size-based enrichment of white blood cells and CTCs from peripheral blood of metastatic cancer patients using a CTC microfilter previously developed in my group. It indicates that the size and deformability of cell nucleus play a critical role in CTC size-based microfiltration and potentially cancer cell translocating micro constrictions in vivo. The second microfluidic platform can measure the Youngs modulus of cells in a high throughput fashion by applying a micropipette aspiration model in an array of micro constrictions. Using this device, a subtype of cancer cells with a softer mechanical phenotype can be enriched. This subtype of cancer cells shows enhanced invasive-related properties and can be used for further study of metastasis and cancer cell heterogeneity.
