Author: C. Edward Gasque
Publisher: McGraw-Hill Companies
ISBN:
Category : Science
Languages : en
Pages : 580

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Author: C. Edward Gasque
Publisher:
ISBN: 9788185392813
Category : Cytology
Languages : en
Pages : 554

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Author: Suzanne Peterson
Publisher: Academic Press
ISBN: 0123854741
Category : Science
Languages : en
Pages : 652

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Book Description
This manual is a comprehensive compilation of "methods that work" for deriving, characterizing, and differentiating hPSCs, written by the researchers who developed and tested the methods and use them every day in their laboratories. The manual is much more than a collection of recipes; it is intended to spark the interest of scientists in areas of stem cell biology that they may not have considered to be important to their work. The second edition of the Human Stem Cell Manual is an extraordinary laboratory guide for both experienced stem cell researchers and those just beginning to use stem cells in their work. Offers a comprehensive guide for medical and biology researchers who want to use stem cells for basic research, disease modeling, drug development, and cell therapy applications Provides a cohesive global view of the current state of stem cell research, with chapters written by pioneering stem cell researchers in Asia, Europe, and North America Includes new chapters devoted to recently developed methods, such as iPSC technology, written by the scientists who made these breakthroughs

Author: Stefan Surzycki
Publisher: John Wiley & Sons
ISBN: 0470999241
Category : Science
Languages : en
Pages : 246

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Book Description
Human Molecular Biology Laboratory Manual offers a hands-on, state-of-the-art introduction to modern molecular biology techniques as applied to human genome analysis. In eight unique experiments, simple step-by-step instructions guide students through the basic principles of molecular biology and the latest laboratory techniques. This laboratory manual's distinctive focus on human molecular biology provides students with the opportunity to analyze and study their own genes while gaining real laboratory experience. A Background section highlighting the theoretical principles for each experiment. Safety Precautions. Technical Tips. Expected Results. Simple icons indicating tube orientation in centrifuge. Experiment Flow Charts Spiral bound for easy lab use

Author: Sue Carson
Publisher: Academic Press
ISBN: 0128157755
Category : Science
Languages : en
Pages : 294

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Book Description
Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions

Author: K. V. CHAITANYA
Publisher: PHI Learning Pvt. Ltd.
ISBN: 8120348001
Category : Science
Languages : en
Pages : 156

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Book Description
This laboratory guide, intended for undergraduate and postgraduate students, includes techniques and their protocols ranging from microscopy to in vitro protein synthesis. Experiments relating to chromosomes study and identifying the phases of cell division are explained. The book lucidly deals with the extraction and characteri-zation of chromatin and techniques for studying its modifications, the gene methodology for identification of mutation and the methodology for isolation of nucleic acids from all types of organisms, such as viruses, fungi, plants and animals. All the protocols have been explained following step-by-step method. Different types of electrophoresis and their techniques, including blotting techniques and the methodology for stripping of probes from membranes for reusing the blot, have also been dealt with. Protocols on modern molecular biology techniques—PCR, restriction enzyme digest, DNA isolation, cloning and DNA sequencing—add weightage to the book. It also gives necessary knowledge of different types of stains, staining techniques, buffers, reagents and media used in the protocols. To help students prepare for answering viva voce questions, the book includes MCQs based on the discussed techniques.

Author: Gary S. Stein
Publisher: John Wiley & Sons
ISBN: 0470889896
Category : Science
Languages : en
Pages : 439

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Book Description
Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells. Key Features: An authoritative, comprehensive, multimedia training manual for stem cell researchers Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology Perspectives written by the world leaders in the field Introductory chapters will provide background information The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.

Author: Warren D. Dolphin
Publisher: McGraw-Hill Education
ISBN: 9780073532264
Category : Science
Languages : en
Pages : 0

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Book Description
Designed to be used with all majors-level general biology textbooks, the included labs are investigative, using both discovery- and hypothesis-based science methods. Students experimentally investigate topics, observe structure, use critical thinking skills to predict and test ideas, and engage in hands-on learning. By emphasizing investigative, quantitative, and comparative approaches to the topics, the authors continually emphasize how the biological sciences are integrative, yet unique. This manual is an excellent choice for colleges and universities that want their students to experience the breadth of modern biology encouraged them to think for themselves. An instructor's manual, provides detailed advice based on the authors' experience on how to prepare materials for each lab, teachings tips and lesson plans, and questions that can be used in quizzes and practical exams

Author: Eldon Enger
Publisher: McGraw-Hill Education
ISBN: 9780077295257
Category : Science
Languages : en
Pages : 0

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Book Description
Preface: The laboratory gives you the opportunity to "get your hands on biology". You go beyond reading and study and actually enter into the process of doing science. The benefit you derive from the laboratory is directly dependent on two things: first, the care with which you perform the experiments and record your observations and, second, your awareness of the relationship between your observations and the general principles under study. Because of time limitations, we cannot investigate in the slow, methodical (often tedious) way scientist do. However, you will have the opportunity to measure, experiment, observe, and discover for yourself. In general, each laboratory exercise consists of the following: Safety box: alerts you to any hazardous materials -- Objectives: explicitly state what you can expect to accomplish -- Introduction: consist of background information to orient you to the biological concept being explored -- Preview: is a general overview of what you will be doing -- Procedure: provides step-by-step instruction to guide you through the laboratory exercise.--End-of-exercise questions: helps you focus on the important observations and concepts relevant to the day's investigation.

Author: Heather B. Miller
Publisher: Academic Press
ISBN: 0123855454
Category : Science
Languages : en
Pages : 227

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Book Description
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions