Mammalian and Avian Transgenesis - New Approaches PDF Download
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Author: Shirley Pease
Publisher: Springer Science & Business Media
ISBN: 3540284893
Category : Science
Languages : en
Pages : 300
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Book Description
Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
Author: Shirley Pease
Publisher: Springer Science & Business Media
ISBN: 3540284893
Category : Science
Languages : en
Pages : 300
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Book Description
Mammalian and Avian Transgenesis presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
Author: Carlos Lois
Publisher:
ISBN: 9786610608713
Category : Birds
Languages : en
Pages : 281
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Book Description
"Mammalian and Avian Transgenesis" presents a collection of novel methods for the production of a wide range of transgenic animals. The manual focuses largely on mice, but also contains protocols for successful transgenesis in rats, cows, pigs and birds. The manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, the use of reporter genes in transgenesis and transgenesis in large animals and birds. The text is supplemented by superb color photos. While the focus is on newly established techniques, the fundamental methods of transgenesis are also covered for those new to the field. Thus this manual is perfectly suited for those wishing to adopt new technologies in transgenesis.
Author: Robert J. Etches
Publisher: Routledge
ISBN: 1351434004
Category : Nature
Languages : en
Pages : 355
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Book Description
Many genes have been cloned from chicken cells, and during the next decade numerous laboratories will be concentrating their resources in developing ways of using these tools. Manipulation of the Avian Genome contains the most recent information from leading research laboratories in the areas of developmental and molecular genetics of the chicken. This information was presented at the Keystone Symposium held at Lake Tahoe in March, 1991. The book discusses potential applications of emerging technology in basic science and poultry production. Various techniques for altering genomic DNA, such as microinjection, retroviral vectors, and lipofection are covered. Genome evaluation using DNA fingerprinting and conventional breeding techniques are presented.
Author: Bernd Kaspers
Publisher: Academic Press
ISBN: 0123972728
Category : Medical
Languages : en
Pages : 456
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Book Description
The second edition of Avian Immunology provides an up-to-date overview of the current knowledge of avian immunology. From the ontogeny of the avian immune system to practical application in vaccinology, the book encompasses all aspects of innate and adaptive immunity in chickens. In addition, chapters are devoted to the immunology of other commercially important species such as turkeys and ducks, and to ecoimmunology summarizing the knowledge of immune responses in free-living birds often in relation to reproductive success. The book contains a detailed description of the avian innate immune system, encompassing the mucosal, enteric, respiratory and reproductive systems. The diseases and disorders it covers include immunodepressive diseases and immune evasion, autoimmune diseases, and tumors of the immune system. Practical aspects of vaccination are examined as well. Extensive appendices summarize resources for scientists including cell lines, inbred chicken lines, cytokines, chemokines, and monoclonal antibodies. The world-wide importance of poultry protein for the human diet, as well as the threat of avian influenza pandemics like H5N1 and heavy reliance on vaccination to protect commercial flocks makes this book a vital resource. This book provides crucial information not only for poultry health professionals and avian biologists, but also for comparative and veterinary immunologists, graduate students and veterinary students with an interest in avian immunology. With contributions from 33 of the foremost international experts in the field, this book provides the most up-to-date review of avian immunology so far Contains a detailed description of the avian innate immune system reviewing constitutive barriers, chemical and cellular responses; it includes a comprehensive review of avian Toll-like receptors Contains a wide-ranging review of the "ecoimmunology" of free-living avian species, as applied to studies of population dynamics, and reviews methods and resources available for carrying out such research
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309070864
Category : Nature
Languages : en
Pages : 348
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Book Description
Scientific Frontiers in Developmental Toxicology and Risk Assessment reviews advances made during the last 10-15 years in fields such as developmental biology, molecular biology, and genetics. It describes a novel approach for how these advances might be used in combination with existing methodologies to further the understanding of mechanisms of developmental toxicity, to improve the assessment of chemicals for their ability to cause developmental toxicity, and to improve risk assessment for developmental defects. For example, based on the recent advances, even the smallest, simplest laboratory animals such as the fruit fly, roundworm, and zebrafish might be able to serve as developmental toxicological models for human biological systems. Use of such organisms might allow for rapid and inexpensive testing of large numbers of chemicals for their potential to cause developmental toxicity; presently, there are little or no developmental toxicity data available for the majority of natural and manufactured chemicals in use. This new approach to developmental toxicology and risk assessment will require simultaneous research on several fronts by experts from multiple scientific disciplines, including developmental toxicologists, developmental biologists, geneticists, epidemiologists, and biostatisticians.
Author: Thomas L. Saunders
Publisher: Springer Nature
ISBN: 1071629905
Category : Science
Languages : en
Pages : 452
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Book Description
This detailed volume focuses on genotyping and validation in addition to information on how to produce gene edited cells and animals for research. Future advances in biomedical research will benefit greatly from the use of precise gene targeting of transgenes in the genome as CRISPR technology supersedes earlier methods that relied on random transgene integration, which this collection reflects. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Transgenesis: Methods and Protocols serves as an ideal guide for researchers working with or working on genetically modified models.
Author: John P. Mulhall
Publisher: Cambridge University Press
ISBN: 1139619527
Category : Medical
Languages : en
Pages : 365
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Book Description
Men with cancer rendered infertile by surgery, chemotherapy, radiation and hormone therapy that are needed to control or cure their disease are increasingly being offered the chance to preserve their reproductive potential through artificial reproductive technologies. Cryopreservation of sperm and testicular tissue have increasingly helped boys and men preserve their fertility. There is a growing subspecialty within reproductive medicine aimed at fertility preservation in this population. Furthermore, strategies are being developed that may in the future revolutionize the approach to such patients. Written by international authorities in the field of fertility preservation, this comprehensive book is aimed at clinicians dealing with male cancer patients, in particular, urologists, andrologists, oncologists, pediatricians and nursing staff as well as clinicians in reproductive endocrinology. The text reviews the impact of cancers and their treatment on male fertility, the available fertility preservation strategies and post-treatment management.
Author: Louis-Marie Houdebine
Publisher: CRC Press
ISBN: 1000448436
Category : Nature
Languages : en
Pages : 122
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Book Description
During the past 20 years, transgenesis has become a popular technique and a crucial tool for molecular geneticists and biologists. Transgene expression is now better-controlled and even specifically inducible by exogenous factors. While these techniques have quite significantly transformed the experimental approaches taken by biologists, the applications are more limited than expected and concerns have arisen regarding biosafety as well as physiological, social, and philosophical issues. Transgenic Animals: Generation and Use contains articles on the techniques used to generate transgenic animals and a section on the preparation of vectors for the optimally controlled expression of transgenes. It also examines the use of transgenic animals in the study of gene function and human diseases, the preparation of recombinant proteins and organs for pharmaceutical and medical use, and the improvement of genetic characteristics of farm animals. Finally, it discusses more recent problems generated by transgenic animals including conservation of transgenic lines, specific database patenting, biosafety, and bioethics. Drawn from both academia and industry, the contributors to this monograph present in one concise volume all the relevant information on the different aspects of transgenesis. This book can be used as both a reference book and a textbook for specialized university courses and will be of interest to everyone involved in basic research in animal biology, molecular genetics, animal biotechnology, pharmaceutical science, and medicine.
Author:
Publisher:
ISBN:
Category : Mammals
Languages : en
Pages : 432
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Book Description
Author: Richard Twyman
Publisher: Garland Science
ISBN: 0203489233
Category : Science
Languages : en
Pages : 299
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Book Description
Gene transfer to animal cells was first achieved more than thirty years ago. Since then, transformation technology has developed rapidly, resulting in a multitude of techniques for cell transformation and the creation of transgenic animals. As with any expanding technology, it becomes difficult to keep track of all the developments and to find a concise and comprehensive source of information that explains all the underlying principles. Gene Transfer to Animals Cells addresses this problem by describing the principles behind gene transfer technologies, how gene expression is controlled in animal cells and how advanced strategies can be used to add, exchange or delete sequences from animal genomes in a conditional manner. A final chapter provides an overview of all the applications of animal cell transformation in farming, medicine and research.
