Low-Cost Methods for Molecular Characterization of Mutant Plants PDF Download
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Author: Bradley J. Till
Publisher: Springer
ISBN: 3319162594
Category : Science
Languages : en
Pages : 42
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Book Description
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Author: Bradley J. Till
Publisher: Springer
ISBN: 3319162594
Category : Science
Languages : en
Pages : 42
Get Book Here
Book Description
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Author: Joanna Jankowicz-Cieslak
Publisher: Springer
ISBN: 3319450212
Category : Science
Languages : en
Pages : 343
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Book Description
This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
Author: Sharon K. Lee
Publisher:
ISBN:
Category : Plant mutation breeding
Languages : en
Pages : 178
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Book Description
Author: Claudius E. Tapia
Publisher:
ISBN: 9781109797909
Category : Plant biotechnology
Languages : en
Pages : 36
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Book Description
The thesis consists of two chapters, and within each chapter is a different set of methods and techniques that will be useful to me in future research endeavors. The first is the transformation of tobacco chloroplast and the analysis of a resultant chloroplast mutant, and the second is the establishment of a regeneration system for the aquatic plant Typha domingensis, commonly known as cattail. The unifying theme is plant transformation. The establishment of a regeneration system for a potentially beneficial plant is useful for future transformations and the actual transformation and analysis of mutants is useful for the characterization of transformants. The chloroplast transformation was unsuccessful and analysis of the mutation demonstrated it as a null mutation under normal growth conditions. Cattail seeds were induced to form calli, then induced to regenerate into normal plants.
Author: Food and Agriculture Organization of the United Nations
Publisher: Food & Agriculture Org.
ISBN: 9251305269
Category : Technology & Engineering
Languages : en
Pages : 319
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Book Description
This paper provides guidelines for new high-throughput screening methods – both phenotypic and genotypic – to enable the detection of rare mutant traits, and reviews techniques for increasing the efficiency of crop mutation breeding.
Author: Q. Y. Shu
Publisher: CABI
ISBN: 1780640854
Category : Nature
Languages : en
Pages : 612
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Book Description
Abstract: This book presents contemporary information on mutagenesis in plants and its applications in plant breeding and research. The topics are classified into sections focusing on the concepts, historical development and genetic basis of plant mutation breeding (chapters 1-6); mutagens and induced mutagenesis (chapters 7-13); mutation induction and mutant development (chapters 14-23); mutation breeding (chapters 24-34); or mutations in functional genomics (chapters 35-41). This book is an essential reference for those who are conducting research on mutagenesis as an approach to improving or modifying a trait, or achieving basic understanding of a pathway for a trait --.
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309166152
Category : Science
Languages : en
Pages : 254
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Book Description
Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.
Author: Rajeev K. Varshney
Publisher: Springer
ISBN: 3319913131
Category : Science
Languages : en
Pages : 306
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Book Description
This book reviews the latest advances in multiple fields of plant biotechnology and the opportunities that plant genetics, genomics and molecular biology have offered for agriculture improvement. Advanced technologies can dramatically enhance our capacity in understanding the molecular basis of traits and utilizing the available resources for accelerated development of high yielding, nutritious, input-use efficient and climate-smart crop varieties. In this book, readers will discover the significant advances in plant genetics, structural and functional genomics, trait and gene discovery, transcriptomics, proteomics, metabolomics, epigenomics, nanotechnology and analytical & decision support tools in breeding. This book appeals to researchers, academics and other stakeholders of global agriculture.
Author: A. Karp
Publisher: Bioversity International
ISBN: 929043323X
Category : Germplasm resources, Plant
Languages : en
Pages : 47
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Book Description
Proceedings of a workshop on the use of molecular techniques in the conservation of plant genetic resources.
Author: Robert J. Henry
Publisher: John Wiley & Sons
ISBN: 1118472993
Category : Science
Languages : en
Pages : 224
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Book Description
Molecular Markers in Plants surveys an array of technologies used in the molecular analysis of plants. The role molecular markers play in plant improvement has grown significantly as DNA sequencing and high-throughput technologies have matured. This timely review of technologies and techniques will provide readers with a useful resource on the latest molecular technologies. Molecular Markers in Plants not only reviews past achievements, but also catalogs recent advances and looks forward towards the future application of molecular technologies in plant improvement. Opening chapters look at the development of molecular technologies. Subsequent chapters look at a wide range of applications for the use of these advances in fields as diverse as plant breeding, production, biosecurity, and conservation. The final chapters look forward toward future developments in the field. Looking broadly at the field of molecular technologies, Molecular Markers in Plants will be an essential addition to the library of every researcher, institution, and company working in the field of plant improvement.
