LOCAL ADAPTATION OF MICROBIAL COMMUNITIES ALONG GEOCHEMICAL SPATIAL GRADIENTS IN SEDIMENTS OF THE LAKE ERIE REGION PDF Download
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Author: Matthew John Hoostal
Publisher:
ISBN:
Category : Erie, Lake
Languages : en
Pages : 189
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Lake Erie demonstrates the greatest productivity of the Laurentian Great Lakes, yet has been critically impacted by anthropogenic activities throughout the Lake Erie watershed. Lake Erie is comprised of three major basins, with east-to-west gradients of increasing drainage areas, increasing riverine inputs of nutrients and xenobiotics, as well as decreasing depth. These large-scale geochemical gradients may be expected to result in spatial patterns of microbial community composition, nutrient cycling, and xenobiotic transformation. As such, Lake Erie provides an excellent system to examine the local adaptation of microbial communities throughout a large freshwater ecosystem. Spatial patterns of microbial community composition, as well as functional diversity, across the three basins of Lake Erie were assessed to examine the potential adaptation of microbial communities to local selective pressures. Community composition was investigated through the generation of 16S rDNA libraries, while functional diversity was evaluated with substrate-induced respiration (SIR) and extracellular enzyme activities (EEA) profiles. EEA profiles were subsequently measured to examine microbial community resilience to metal inoculations in sediments contaminated with heavy metals compared to relatively pristine sediments. Bioinformatic studies of bacterial genes involved in the efflux of heavy metals from the cell were performed to provide a conceptual framework of how horizontal gene transfer may expedite the adaptation of bacterial communities to heavy metal stress. Finally, the local adaptation of bacterial communities to PCBs and PAHs was assessed by comparing the diversity of bphA, a gene that initiates PCB metabolism, in polluted and relatively unpolluted sediments within the Lake Erie watershed. Collectively, results suggested large-scale spatial patterns of microbial community composition, functional diversity, and metabolic resilience consistent with the local adaptation of sediment bacterial communities to allochthonous inputs of organic matter and heavy metal pollutants into Lake Erie. Furthermore, estimates of diversity from bphA environmental gene libraries suggest that PCB and PAH contamination represents a driving force in the adaptation of microbial communities in polluted sediments. Results from this study suggest that microbial communities are highly integrated assemblages of multiple taxa locally adapted to differential inputs of nutrients and xenobiotics across geochemical gradients within freshwater ecosystems.
Author: Matthew John Hoostal
Publisher:
ISBN:
Category : Erie, Lake
Languages : en
Pages : 189
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Book Description
Lake Erie demonstrates the greatest productivity of the Laurentian Great Lakes, yet has been critically impacted by anthropogenic activities throughout the Lake Erie watershed. Lake Erie is comprised of three major basins, with east-to-west gradients of increasing drainage areas, increasing riverine inputs of nutrients and xenobiotics, as well as decreasing depth. These large-scale geochemical gradients may be expected to result in spatial patterns of microbial community composition, nutrient cycling, and xenobiotic transformation. As such, Lake Erie provides an excellent system to examine the local adaptation of microbial communities throughout a large freshwater ecosystem. Spatial patterns of microbial community composition, as well as functional diversity, across the three basins of Lake Erie were assessed to examine the potential adaptation of microbial communities to local selective pressures. Community composition was investigated through the generation of 16S rDNA libraries, while functional diversity was evaluated with substrate-induced respiration (SIR) and extracellular enzyme activities (EEA) profiles. EEA profiles were subsequently measured to examine microbial community resilience to metal inoculations in sediments contaminated with heavy metals compared to relatively pristine sediments. Bioinformatic studies of bacterial genes involved in the efflux of heavy metals from the cell were performed to provide a conceptual framework of how horizontal gene transfer may expedite the adaptation of bacterial communities to heavy metal stress. Finally, the local adaptation of bacterial communities to PCBs and PAHs was assessed by comparing the diversity of bphA, a gene that initiates PCB metabolism, in polluted and relatively unpolluted sediments within the Lake Erie watershed. Collectively, results suggested large-scale spatial patterns of microbial community composition, functional diversity, and metabolic resilience consistent with the local adaptation of sediment bacterial communities to allochthonous inputs of organic matter and heavy metal pollutants into Lake Erie. Furthermore, estimates of diversity from bphA environmental gene libraries suggest that PCB and PAH contamination represents a driving force in the adaptation of microbial communities in polluted sediments. Results from this study suggest that microbial communities are highly integrated assemblages of multiple taxa locally adapted to differential inputs of nutrients and xenobiotics across geochemical gradients within freshwater ecosystems.
Author: Anna Kathleen Ormiston
Publisher:
ISBN:
Category : Cyanobacteria
Languages : en
Pages : 103
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Through close interactions with biotic and abiotic environments, microbial communities in lakes mediate numerous biogeochemical processes that are essential in regional and global cycles of C, N and P. However, the relationship between bacterial community compositions and environmental conditions is still unclear. Lake Erie's natural gradient of nutrient supply and many other environmental parameters from the Sandusky Bay to the Central Basin provides an ideal experiment to examine how well bacterial community composition tracks environmental changes spatially and temporally. Surface water samples were collected along a transect that ran from the Sandusky Bay (hypereutrophic) via Sandusky Sub-basin (mesoeutrophic) to the Central Basin (oligotrophic) in June, July and August 2012. Zooplankton sample were also collected at each basin in June, July and August to see whether they respond to environmental conditions and to the changing bacterioplankton communities. Physico-chemical parameters were measured in situ. Bacterioplankton was collected on filters and filtrates were used for nutrient analyses, including ammonium, dissolved organic carbon, total dissolved nitrogen, nitrate, nitrite and soluble reactive phosphorus. Chlorophyll a concentration measurements confirmed the expected gradient of primary productivity among sites. Terminal restriction fragment length polymorphism (T-RFLP) analysis was conducted to compare of the microbial community structure and diversity along this natural gradient from the Sandusky Bay to the Central Basin. Additionally, zooplankton community structure and diversity was compared along the transect. Results showed that the free-living bacterioplankton structure differed significantly among sampling time, which was likely contributed by temporal variations in nutrient concentrations. As for the zooplankton community, Cyclopidae, Branchionidae and Synchaetidae were identified as major families (>78.4% of total zooplankton) in all samples. Zooplankton family structure had no clear separation based on site location on site location or sampling time. In addition, no significant correlation was identified between zooplankton community structure and environmental parameters or with zooplankton community structure and bacterioplankton community structure. Zooplankton diversity tests revealed significant differences in zooplankton diversity among sites and months. This research contributes a better understanding of the zooplankton and bacteria community structure found in Lake Erie. Along with this natural nutrient gradient found in Lake Erie, harmful cyanobacterial blooms (cyanoHABS) is also a serious issue that affects wildlife, human health, recreation and local economics. CyanoHABs produce cyanotoxins, such as microcystins that lead to skin irritation, illness and liver tumors. Natural bacterial degradation of these microcystins play a key role in lakes by transforming these harmful toxins to less harmful metabolites that can be consumed by other organisms without a detrimental affect on their health and ecosystem health. Microcystin-LR is a toxin produced in harmful cyanobacteria blooms in Lake Erie and in Grand Lake St. Marys. This experiment specifically compared bacterial community structure and diversity from lakes with previous CyanoHAB exposure and their response to amended Microcystin-LR levels. Water samples were collected in June 2012 in the western basin, Sandusky sub-basin and central basin of Lake Erie and three recreational sites in Grand Lake St. Mary’s. Particulate-associated bacterioplankton was filtered out of the water samples, and the remaining filtrate was starved of all carbon and incubated in the dark for 1 week. After incubation, water samples were divided into triplicate microcosms. Microcystin-LR additions were added to the water samples as the sole carbon source for the naturally existing bacterioplankton community. After the microcystin-LR addition cell counts and microcystin concentrations were measure every 24 hours for two days. T-RFLP analysis was conducted to compare original bacterial community structure and diversity for each site to the Microcystin-LR amended bacterial community structure and diversity. Significant differences between start and end MC-LR concentrations (p 0.05) measured in the incubation experiment indicated MC-LR degradation. Shannon diversity indices for bacterioplankton T-RF percent abundances were not significantly different between treatments for both lakes (ANOVA, p 0.05). T-RFLP results showed that bacterioplankton community structures were significantly different between microcystin amended and original free-living bacterioplankton communities for Grand Lake St. Mary samples, but there was no significant difference between community structure for MC-amended treatments and non amended controls. In contrast, Lake Erie’s MC-amended communities experienced no shift in community structure. Non-amended controls had natural occurring MC-concentration, which suggests that there is a large subset of bacterioplankton that could degrade MC-LR before the treatments were administered. The extensive CyanoHAB history found in both lakes can explain these results. There are two dominating cyanobacterial species in Grand Lake St. Mary’s and four in Lake Erie, which suggests that these differences may affect the differences in MC degraders found in both lakes and the overall bacterioplankton community structure. Evidence of MC-degradation could be explained by bacterioplankton using MC-LR as an energy source.
Author:
Publisher:
ISBN:
Category : Beach closures
Languages : en
Pages : 40
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Author: Chloé Shoshana Jessica Orland
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ISBN:
Category :
Languages : en
Pages :
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Author: Matti Olavi Ruuskanen
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Since the Industrial Revolution at the end of the 18th century, anthropogenic changes in the environment have shifted from the local to the global scale. Even remote environments such as the high Arctic are vulnerable to the effects of climate change. Similarly, anthropogenic mercury (Hg) has had a global reach because of atmospheric transport and deposition far from emission point sources. Whereas some effects of climate change are visible through melting permafrost, or toxic effects of Hg at higher trophic levels, the often-invisible changes in microbial community structures and functions have received much less attention. With recent and drastic warming-related changes in Arctic watersheds, previously uncharacterized phylogenetic and functional diversity in the sediment communities might be lost forever. The main objectives of my thesis were to uncover how microbial community structure, functional potential and the evolution of mercury specific functions in lake sediments in northern latitudes (>54{486}N) are affected by increasing temperatures and Hg deposition. To address these questions, I examined environmental DNA from sediment core samples and high-throughput sequencing to reconstruct the community composition, functional potential, and evolutionary responses to historical Hg loading. In my thesis I show that the microbial community in Lake Hazen (NU, Canada) sediments is structured by redox gradients and pH. Furthermore, the microbes in this phylogenetically diverse community contain genomic features which might represent adaptations to the cold and oligotrophic conditions. Finally, historical Hg pollution from anthropogenic sources has likely affected the evolution of microbial Hg resistance and this deposition can be tracked using sediment DNA on the Northern Hemisphere. My thesis underscores the importance of using culture-independent methods to reconstruct the structure, functional potential and evolution of environmental microbial communities.
Author: Philips Olugbemiga Akinwole
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 168
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Sedimentary microbial communities play a critical ecological role in lotic ecosystems and are responsible for numerous biogeochemical transformations, including dissolved organic matter (DOM) uptake, degradation, and mineralization. The goals of this study were to elucidate the benthic microbes responsible for utilization of humic DOM in streams and to assess overall variability in microbial biomass and community structure over time and across multiple spatial scales in stream networks, as DOM quality and quantity will likely change with stream order. In Chapter 2, multiple spatial patterns of microbial biomass and community structure were examined in stream sediments from two watersheds; the Neversink River watershed (NY; 1st, 3rd and 5th order streams sampled) and the White Clay Creek watershed (PA; 1st through 3rd order streams sampled). Microbial biomass and community structure were estimated by phospholipid phosphate and phospholipid fatty acids (PLFA) analyses. Multivariate analysis showed that sedimentary C:N ratios, percent carbon, sediment surface area and percent water content explained 68% of the variations in total microbial biomass. Overall, the magnitude of within stream variation in microbial biomass was small compared to the variability noted among streams and between watersheds. Principal component analysis (PCA) of PLFA profiles showed that microbial community structure displayed a distinct watershed-level biogeography, as well as variation along a stream order gradient. Chapter 3 demonstrated that benthic microbial biomass was seasonally dynamic and significantly correlated to a combination of high and low flood pulse counts, variability in daily flow and DOC concentration in the White Clay Creek. Additionally, the seasonal pattern of variation observed in microbial community structure was as a result of shift between the ratios of prokaryotic to eukaryotic component of the community. This shift was significantly correlated with seasonal changes in median daily flow, high and low flood pulse counts, DOC concentrations and water temperature. Compound-specific 13C analysis of PLFA showed that both bacterial and microeukaryotic stable carbon isotope ratios were heaviest in the spring and lightest in autumn or winter. Bacterial lipids were isotopically depleted on average by 2 - 5 / relative to δ13C of total organic carbon suggesting bacterial consumption of allochthonous organic matter, and enriched relative to δ13C algae-derived carbon source. In Chapter 4, heterotrophic microbes that metabolize humic DOM in a third-order stream were identified through trace-additions of 13C-labeled tree tissue leachate (13C-DOC) into stream sediment mesocosms. Microbial community structure was assessed using PLFA biomarkers, and metabolically active members were identified through 13C-PLFA analysis (PLFA-SIP). Comparison by PCA of the microbial communities in stream sediments and stream sediments incubated in both the presence and absence of 13C-DOC showed our mesocosm-based experimental design as sufficiently robust to investigate the utilization of 13C-DOC by sediment microbial communities. After 48 hours of incubation, PLFA-SIP identified heterotrophic α, β, and γ- proteobacteria and facultative anaerobic bacteria as the organisms primarily responsible for humic DOC consumption in streams and heterotrophic microeucaryotes as their predators. The evidence presented in this study shows a complex relationship between microbial community structure, environmental heterogeneity and utilization of humic DOC, indicating that humic DOC quality and quantity along with other hydro-ecological variables should be considered among the important factors that structure benthic microbial communities in lotic ecosystems.
Author: Robert Proctor Apmann
Publisher:
ISBN:
Category : Erie, Lake
Languages : en
Pages : 200
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This paper investigates historical trends in pollutant loadings to Lake Erie. The first of two sections of this report analyzes information on the amount of sediments delivered to Lake Erie and discusses historical trends in sediment yield. There are two primary sources of data: (1) measurements of suspended sediment load made in some of the tributary rivers and (2) analyses of shoreline erosion and sedimentation rates in Lake Erie. These lead to a subsequent analysis of the sediment budget. Since the loading of contaminants to the lake is manifested by sedimentation, results are presented of analyses of contamination inputs from bottom coring. Trends in precipitation, runoff, and lake levels have been analyzed and are presented followed by the calculation and tabulation of the average surface water runoff to Lake Erie. The second section of the report deals primarily with the sources and inputs of the two nutrients, phosphorus and nitrogen, to Lake Erie. There is little specific information on those inputs, and, therefore, information has been assembled from other locations on the sources of these nutrients. The historical growth in population in the Lake Erie Basin has also been traced.
Author: Ralph P. Collins
Publisher:
ISBN:
Category : Water
Languages : en
Pages : 52
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Periodic taste and odor at the Cleveland, Ohio Crown Water Treatment Plant prompted investigation of the role microorganisms play in the problem. Fungi, bacteria, and algae collected near the plant intake were studied during June through August 1971. During the three months of sampling, no vertical distribution pattern was noted in quantitative analysis of the phytoplankton. A number of algae, reported to induce taste and odor in water, were identified. Whatever the source of these odors, they were not due to benthic or periphyton algae, but could have been associated with the phytoplankton community as the reported 'Lake Erie odor' coincided with phytoplankton increase.
Author: Eric Capo
Publisher: Springer Nature
ISBN: 3031437993
Category : Science
Languages : en
Pages : 450
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This book, entitled Tracking Environmental Change Using Lake Sediments: Volume 6 – Sedimentary DNA, provides an overview of the applications of sedimentary DNA-based approaches to paleolimnological studies. These approaches have shown considerable potential in providing information about the long-term changes of overall biodiversity in lakes and their watersheds in response to natural and anthropogenic changes, as well as tracking human migrations over the last thousands of years. Although the first studies investigating the preservation of these molecular proxies in sediments originate from the late-1990s, the number of scientific publications on this topic has increased greatly over the last five years. Alongside numerous ecological findings, several sedimentary DNA studies have been dedicated to understanding the reliability of this approach to reconstruct past ecosystem changes. Despite the major surge of interest, a comprehensive compilation of sedimentary DNA approaches and applications has yet to be attempted. The overall aim of this DPER volume is to fill this knowledge gap.
Author: Lester J. Walters
Publisher:
ISBN:
Category : Acid pollution of rivers, lakes, etc
Languages : en
Pages : 186
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