Techniques In Microscopy For Biomedical Applications PDF Download
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Author: Terje Dokland
Publisher: World Scientific Publishing Company
ISBN: 9813101954
Category : Technology & Engineering
Languages : en
Pages : 422
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Book Description
The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues.Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences.The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.
Author: Terje Dokland
Publisher: World Scientific Publishing Company
ISBN: 9813101954
Category : Technology & Engineering
Languages : en
Pages : 422
Get Book Here
Book Description
The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues.Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences.The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.
Author: J. James
Publisher: Springer Science & Business Media
ISBN: 9401014140
Category : Medical
Languages : en
Pages : 345
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Book Description
Up to about twenty-five years ago, virtually the entire field of microscopy could be overseen and even practized by any active research worker. The rapid evolution which microscopy in its broadest sense has since undergone and which has contributed greatly to our insight in many fields of biological science and medicine has, however, lead to a progressive specialisation. Both experienced investigators in clinical and biological laboratories and post graduate students, confronted with a limited number of microscopic tech niques in their daily research work, have increasing difficulty in keeping (or obtaining) a general idea of the many time-honoured and new possibilities which microscopy has to offer. This book has been written with the aim of presenting general informa tion on light microscopic techniques, at a level somewhere in between booklets like those provided by microscope manufacturers (which are often too much focussed on the production program of a particular make) and very advanced treatises with a thorough mathematical treatment of all phenomena concerned. The physically oriented texts moreover often do not sufficiently take into account the practical situation in a medical or biolog ical laboratory; on the other hand, the value of really understanding what one is doing in using a microscopic technique is often underestimated. At tempt has been made, therefore, to present sufficient background informa tion necessary for a rational application of the different microscopical tech niques in their mutual relationship.
Author: Jens Rietdorf
Publisher: Springer Science & Business Media
ISBN: 9783540236986
Category : Science
Languages : en
Pages : 344
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Book Description
With contributions by numerous experts
Author: James Pawley
Publisher: Springer Science & Business Media
ISBN: 1475753489
Category : Science
Languages : en
Pages : 639
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Book Description
This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging. Coverage includes practical multiphoton, photodamage and phototoxicity, 3D FRET, 3D microscopy correlated with micro-MNR, CARS, second and third harmonic signals, ion imaging in 3D, scanning RAMAN, plant specimens, practical 3D microscopy and correlated optical tomography.
Author: Douglas B. Murphy
Publisher:
ISBN:
Category :
Languages : en
Pages : 538
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Book Description
Author: David L. Spector
Publisher: CSHL Press
ISBN: 0879697512
Category : Science
Languages : en
Pages : 384
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Book Description
This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.
Author: Terence David Allen
Publisher: Oxford University Press, USA
ISBN: 0198701268
Category : Science
Languages : en
Pages : 153
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Book Description
"Using light, electrons, or X-rays, microscopes today form a vital tool not only in biology but in many other disciplines, including materials science and nanotechnology. In this Very Short Introduction Terence Allen describes the scientific principles behind the main forms of microscopy, and the exciting new developments in the field. Beginning with a brief history of microscopy, Allen surveys the diverse and powerful forms of microscopes available today, illustrating how microscopy impinges on almost every aspect of our daily lives."--Inside front cover.
Author: Douglas J. Taatjes
Publisher: Springer Science & Business Media
ISBN: 1592599931
Category : Medical
Languages : en
Pages : 505
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Book Description
A diverse collection of state-of-the-art methods for the microscopic imaging of cells and molecules. The authors cover a wide spectrum of complimentary techniques, including such methods as fluorescence microscopy, electron microscopy, atomic force microscopy, and laser scanning cytometry. Additional readily reproducible protocols on confocal scanning laser microscopy, quantitative computer-assisted image analysis, laser-capture microdissection, microarray image scanning, near-field scanning optical microscopy, and reflection contrast microscopy round out this eclectic collection of cutting-edge imaging techniques now available. The authors also discuss preparative methods for particles and cells by transmission electron microscopy.
Author: Ch.W. Sensen
Publisher: Springer Science & Business Media
ISBN: 3540689931
Category : Medical
Languages : en
Pages : 447
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Book Description
A picture says more than a thousand words. This is something that we all know to be true. Imaging has been important since the early days of medicine and bi- ogy, as seen in the anatomical studies of Leonardo Da Vinci or Andreas Vesalius. More than 100 years ago, the ?rst noninvasive imaging technologies, such as K- rad Roentgen’s X-ray technology,were applied to the medical ?eld—and while still crude—revolutionized medical diagnosis. Today, every patient will be exposed to some kind of advanced imaging technology such as medical resonance imaging, computed tomography or four-dimensional ultrasound during their lifetime. Many diseases, such as brain tumors, are initially diagnosed solely by imaging, and most of the surgical planning relies on the patient imagery. 4D ultrasound is available to expecting parents who wish to create unique early memories of the new baby, and it may soon be used for the morphometric diagnosis of malformations that may one day be treatable—inutero! Light and electron microscopy are unequal brethren, which have contributed to most of our knowledge about the existence and organization of cells, tissues and microorganisms. Every student of biology or medicine is introduced to the fascinating images of the microcosm. New advances have converted these im- ing technologies, which were considered by many to be antiquated, into powerful tools for research in systems biology and related ?elds.
Author: Robert D. Goldman
Publisher: CSHL Press
ISBN: 9780879696832
Category : Science
Languages : en
Pages : 658
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Book Description
Recent advances in imaging technology reveal, in real time and great detail, critical changes in living cells and organisms. This manual is a compendium of emerging techniques, organized into two parts: specific methods such as fluorescent labeling, and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles, organs, and whole animals. Although presented primarily as a laboratory manual, the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action, created by investigators using the imaging techniques discussed in the book. The editors, David Spector and Robert Goldman, whose previous book was Cells: A Laboratory Manual,are highly respected investigators who have taught microscopy courses at Cold Spring Harbor Laboratory, the Marine Biology Laboratory at Woods Hole, and Northwestern University.
