Author: Richa Salwan
Publisher: Elsevier
ISBN: 0323950795
Category : Science
Languages : en
Pages : 208
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Book Description
Laboratory Methods in Microbiology and Molecular Biology describes various microbiological, biochemical, and molecular methods employed for the characterization, identification, and analysis of actinomycetes, bacteria and fungi. The book details general guidelines, expectations, and responsibilities for good lab practices and consists of chapters that covers basic microbiological, physiological, biochemical, and molecular aspects as well as in silico analysis using various bioinformatic tools. Other topics in the book include how to make solutions, microscopy and imaging of microorganisms, sero-diagnostics, and basic concepts of phylogeny, physiology, biotechnology, soil, food, and environmental microbiology while working in laboratory. Laboratory Methods in Microbiology and Molecular Biology is an informative update to current practices and future perspectives for the field of microbial biotechnology. It aims to facilitate professors, researchers, and graduate students in monitoring the precision and accuracy of the qualitative and quantitative methods in their research. Involves various procedures in diverse disciplines, from microbiology to genetics, molecular biology and biochemistry Lists the principles and facts underlying practical applications of bacteria and fungi which have prospects in various technologies Includes the questions ‘how’ and ‘why’ as an explanation for novice students and researchers to modify protocols Facilitates students, teachers and researchers to monitor the precision and accuracy of their qualitative and quantitative methods practically
Author: Heather Miller
Publisher: Academic Press
ISBN: 0123855454
Category : Science
Languages : en
Pages : 232
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Book Description
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author: Walt Ream
Publisher: Elsevier
ISBN: 0080479510
Category : Science
Languages : en
Pages : 268
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Book Description
Molecular Microbiology Laboratory is designed to teach molecular biology techniques to upper level undergraduates majoring in the life sciences. An extremely detailed lab preparation manual for teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading, as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles. The studies in these articles employ the techniques that the students are learning in the lab exercises, which reinforces their understanding of the material. These are techniques that students in any biological science will need to know, making this manual applicable to any life science curriculum. Key Features * Not a typical cookbook lab exercise, offers students the excitement and intellectual challenge of characterizing true unknowns. They could discover a new species! * Success rate greater than 85% for the entire experiment, even with very inexperienced students. * The ONLY manual that incorporates writing exercises into the curriculum. * Co-authored by Dr. Janine Trempy, one of four senior editors of the Journal of Microbiology Education, published by the American Society for Microbiology.
Author: W. F. Harrigan
Publisher: Academic Press
ISBN: 1483274349
Category : Science
Languages : en
Pages : 375
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Book Description
Laboratory Methods in Microbiology is a laboratory manual based on the experience of the authors over several years in devising and organizing practical classes in microbiology to meet the requirements of students following courses in microbiology at the West of Scotland Agricultural College. The primary object of the manual is to provide a laboratory handbook for use by students following food science, dairying, agriculture and allied courses to degree and diploma level, in addition to being of value to students reading microbiology or general bacteriology. It is hoped that laboratory workers in the food manufacturing and dairying industries will find the book useful in the microbiological aspects of quality control and production development. The book is organized into two parts. Part I is concerned with basic methods in microbiology and would normally form the basis of a first year course. Abbreviated recipes and formulations for a number of typical media and reagents are included where appropriate, so that the principles involved are more readily apparent. Part II consists of an extension of these basic methods into microbiology as applied in the food manufacturing, dairying and allied industries. In this part, the methods in current use are given in addition to, or in place of, the ""classical"" or conventional techniques.
Author: C. A. Reddy
Publisher: American Society for Microbiology Press
ISBN: 1555812236
Category : Science
Languages : en
Pages : 2949
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Book Description
A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. • Provides a comprehensive compendium of methods used in general and molecular microbiology. • Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis. • Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions. • Presents methods for bacteria, archaea, and for the first time a section on mycology. • Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.
Author: Walt Ream
Publisher: Academic Press
ISBN: 0080536824
Category : Science
Languages : en
Pages : 245
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Book Description
This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Provides well-tested experimental protocols for each technique Lists the reagents and preparation of each experiment separately Contains a complete schedule of experiments and the preparation required Includes study questions at the end of each chapter
Author: David H. Persing
Publisher: John Wiley & Sons
ISBN: 1555819079
Category : Science
Languages : en
Pages : 835
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Book Description
Presenting the latest molecular diagnostic techniques in one comprehensive volume The molecular diagnostics landscape has changed dramatically since the last edition of Molecular Microbiology: Diagnostic Principles and Practice in 2011. With the spread of molecular testing and the development of new technologies and their opportunities, laboratory professionals and physicians more than ever need a resource to help them navigate this rapidly evolving field. Editors David Persing and Fred Tenover have brought together a team of experienced researchers and diagnosticians to update this third edition comprehensively, to present the latest developments in molecular diagnostics in the support of clinical care and of basic and clinical research, including next-generation sequencing and whole-genome analysis. These updates are provided in an easy-to-read format and supported by a broad range of practical advice, such as determining the appropriate type and quantity of a specimen, releasing and concentrating the targets, and eliminating inhibitors. Molecular Microbiology: Diagnostic Principles and Practice Presents the latest basic scientific theory underlying molecular diagnostics Offers tested and proven applications of molecular diagnostics for the diagnosis of infectious diseases, including point-of-care testing Illustrates and summarizes key concepts and techniques with detailed figures and tables Discusses emerging technologies, including the use of molecular typing methods for real-time tracking of infectious outbreaks and antibiotic resistance Advises on the latest quality control and quality assurance measures Explores the increasing opportunities and capabilities of information technology Molecular Microbiology: Diagnostic Principles and Practice is a textbook for molecular diagnostics courses that can also be used by anyone involved with diagnostic test selection and interpretation. It is also a useful reference for laboratories and as a continuing education resource for physicians.
Author: John Lammert
Publisher: Pearson
ISBN:
Category : Computers
Languages : en
Pages : 244
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Book Description
This vivid, full-color laboratory techniques handbook is an instructive, concise, graphical presentation of the skills and techniques required in an introductory microbiology lab. Clear visual instructions enable readers to carry out fundamental manipulations and procedures effectively and safely. Demonstrates those techniques that will be used frequently for studying microbes in the laboratory. Has a safety section and frequent safety cautions throughout. Has a convenient, portable 6 x 9 trim size, a spiral binding and soft cover, making it ideal for use on the lab bench surface. It is priced inexpensively so that it will be suitable as a supplement to an in-house or commercial manual. Companion to any introductory laboratory whether for biology majors or allied health majors. "
Author: Robert F. Schleif
Publisher: Springer Science & Business Media
ISBN: 1461259568
Category : Science
Languages : en
Pages : 229
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Book Description
This volume has evolved from a laboratory methods book that one of us first compiled nearly fifteen years ago. Since that time the book has undergone many minor revisions in order to include new methods and updated versions of older methods. The result has been an increasingly useful and more widely circulated book. However, the recent series of technological explosions generally lumped together under the name of "recombinant DNA technology" has been a turning point in the evolution of this previously underground publication. Minor revisions will no longer do. To keep the book useful we have had to make major revi sions and additions. The result is a dramatically expanded book that should be more useful to more people. The larger size and wider usefulness of the book have made this more formal publication seem a reasonable step to take. One of the reasons that this volume should be useful to many people is that it includes only procedures that have been used repeatedly by us and that have proven highly reliable both to ourselves and to others in our laboratories.
Author: Sharad Vats
Publisher: GRIN Verlag
ISBN: 3656871507
Category : Medical
Languages : en
Pages : 221
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Book Description
Document from the year 2014 in the subject Biology - Micro- and Molecular Biology, , language: English, abstract: A laboratory Text book of Biochemistry, Molecular Biology and Microbiology is intended to prepare the undergraduate, postgraduate and research students to perform basic experiments on various aspects of bioscience and biotechnology. Moreover, in the Semester system of teaching it is necessary to explore experiments which are not lengthy and easily completed within contact hours. Initially the book deals with dilutions, pH, buffers, units of measurements and calculations. This is followed by lab safety rules which is very important for any student working with chemicals for their and safety of others. This book emphasizes on principles, reagent preparations and procedures related to experiments, which will be handy for students from different scientific backgrounds. A number of methods are available in the literature for quantification of various molecules. This book does not present all the available methods but based on experience it contains commonly used methods, which students should know. The methods have been written in a manner for direct practical use in the laboratory. This work has originated as a result of numerous requests from my students for eased out and explanatory methods pertaining to biochemistry, biotechnology, microbiology and others. The section on testing of adulterants is of much use for common mass because most of the food products we eat are adulterated. The approach is rather simple with the use of very easily available chemicals and the tests can be performed even in house. It is hoped that the reliable assays presented in this manual will help the students and research scholars to get to basics of experiments and various aspects associated with it.
