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Author:
Publisher: Elsevier
ISBN: 0124199542
Category : Science
Languages : en
Pages : 405
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Book Description
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on DNA
Author:
Publisher: Elsevier
ISBN: 0124199542
Category : Science
Languages : en
Pages : 405
Get Book Here
Book Description
Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on DNA
Author: Ray Wu
Publisher: Academic Press
ISBN: 0323137741
Category : Science
Languages : en
Pages : 935
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Book Description
The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.* Methods for: * DNA isolation and cloning* Synthesizing complementary DNA (cDNA)* Cleaving and manipulating DNA * Selecting useful reporter genes* Constructing vectors for cloning genes* Constructing expression vectors* Site-directed mutagenesis and gene disruption* Identifying and mapping genes* Transforming animal and plant cells* Sequencing DNA* Amplifying and manipulating DNA and PCR* Detecting DNA - protein interaction
Author: Richard R Burgess
Publisher: Academic Press
ISBN: 0080923178
Category : Science
Languages : en
Pages : 915
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Book Description
Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. - Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology - Assembles chapters on both common and less common relevant techniques - Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Author:
Publisher: Academic Press
ISBN: 9780121822545
Category : Science
Languages : en
Pages : 735
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Book Description
This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include cytology, biochemistry, cell fractionation, and cell biology.
Author:
Publisher: Academic Press
ISBN: 0128151498
Category : Science
Languages : en
Pages : 460
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Book Description
Volume 608 of the series Methods in Enzymology covers key aspects of enzyme discovery, engineering tools and platforms, and examples of applications in the enzymology of synthetic biology. Detailed methods for laboratory use of enzymes in synthetic biology applications Informative case history examples illustrating how enzyme and metabolic engineering are used to generate new products Emphasises latest developments in laboratory automation for the engineering of biology Covers many aspects of the design, build, test, learn cycle used in synthetic biology
Author: Benjamin F. Lasseter
Publisher: CRC Press
ISBN: 0429957386
Category : Medical
Languages : en
Pages : 203
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Book Description
Most lab manuals assume a high level of knowledge among biochemistry students, as well as a large amount of experience combining knowledge from separate scientific disciplines. Biochemistry in the Lab: A Manual for Undergraduates expects little more than basic chemistry. It explains procedures clearly, as well as giving a clear explanation of the theoretical reason for those steps. Key Features: Presents a comprehensive approach to modern biochemistry laboratory teaching, together with a complete experimental experience Includes chemical biology as its foundation, teaching readers experimental methods specific to the field Provides instructor experiments that are easy to prepare and execute, at comparatively low cost Supersedes existing, older texts with information that is adjusted to modern experimental biochemistry Is written by an expert in the field This textbook presents a foundational approach to modern biochemistry laboratory teaching together with a complete experimental experience, from protein purification and characterization to advanced analytical techniques. It has modules to help instructors present the techniques used in a time critical manner, as well as several modules to study protein chemistry, including gel techniques, enzymology, crystal growth, unfolding studies, and fluorescence. It proceeds from the simplest and most important techniques to the most difficult and specialized ones. It offers instructors experiments that are easy to prepare and execute, at comparatively low cost.
Author: William B. Coleman
Publisher: Springer Science & Business Media
ISBN: 1475725884
Category : Medical
Languages : en
Pages : 387
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Book Description
Notable practitioners describe how laboratory medicine is practiced today and illuminate how it will function tomorrow as the revolutionary advances afforded by molecular diagnostics become increasingly central to effective analysis. Proceeding from a discussion of elementary nucleic acid technology to a review of the more advanced techniques, the distinguished contributors lay the groundwork for a comprehensive understanding of their applications throughout clinical medicine. The result is a detailed description of those molecular technologies currently used in diagnostic laboratories, as well as those that seem particularly promising. Detailed discussions of specific clinical applications include those for cancer, hematological malignancies, cardiovascular disease, and neuromuscular, endocrine, and infectious diseases.
Author: Michael M. Burrell
Publisher: Humana Press
ISBN: 9780896032347
Category : Science
Languages : en
Pages : 370
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Book Description
The scientist' s understanding of the cell at the molecular level has advanced rapidly over the last twenty years. This improved understa- ing has led to the development of many new laboratory methods that increasingly allow old problems to be tackled in new ways. Thus the modern scientist cannot specialize in just one field of knowledge, but must be aware of many disciplines. To aid the process of investigation, the Methods Molecular Biology series has brought together many protocols and has highlighted the useful variations and the pitfalls of the different methods. However, protocols frequently cannot be simply taken from the shelf. Thus the starting sample for a chosen protocol may be unavailable in the correct state or form, or the products of the procedure require a different sort of processing. Therefore the scientist needs more detailed information on the nature and requirements of the enzymes being used. This information, though usually available in the literature, is often widely dispersed and frequently occurs in older volumes of journals; not everyone has comprehensive library facilities available. Also many scientists searching out such information are not trained enzymologists and may be unaware of some of the parameters that are important in a specific enzyme reaction.
Author: Gonzalo de Gonzalo
Publisher: John Wiley & Sons
ISBN: 352734683X
Category : Science
Languages : en
Pages : 54
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Book Description
This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.
Author: Hans Bisswanger
Publisher: John Wiley & Sons
ISBN: 3527820809
Category : Science
Languages : en
Pages : 574
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Book Description
A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.
