Investigations of Combined Gas Chromatography - Mass Spectrometry Techniques for the Analysis of the Sterol Contents of Marine Lipids PDF Download
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Author: John C. Roberts
Publisher:
ISBN:
Category : Gas chromatography
Languages : en
Pages :
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Book Description
Author: John C. Roberts
Publisher:
ISBN:
Category : Gas chromatography
Languages : en
Pages :
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Book Description
Author: J. C. Roberts
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: Angela Lavis
Publisher:
ISBN:
Category : Gas chromatography
Languages : en
Pages :
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Author: Angela Lavis
Publisher:
ISBN:
Category : Gas chromatography
Languages : en
Pages : 0
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Author:
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: R.J. Hamilton
Publisher: Springer Science & Business Media
ISBN: 1461311314
Category : Technology & Engineering
Languages : en
Pages : 406
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Book Description
This book focuses on the developments in the field of lipid analysis, providing an up-to-date review of the analytical techniques available to chemists and technologists to identify complex molecules. The requisite theoretical background will be provided for individual techniques, together with their strengths and weaknesses, and a guide to the enormous range of commercial applications. It will be an invaluable reference source to all sectors of the oils and fats industry where accurate labeling of foods, food contamination and adulteration are issues of increasing interest and concern.
Author: Zheng Yang
Publisher:
ISBN:
Category : Gas chromatography
Languages : en
Pages : 192
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Author: Xu Zeng
Publisher:
ISBN:
Category :
Languages : en
Pages : 380
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Book Description
The determination and accurate identification of fatty acids (FAs) in biological samples remain challenging due to the complexity of these compounds and confounding sample matrix effects. This thesis highlights a range of method developments and applications using advanced multidimensional gas chromatography (MDGC) and comprehensive two dimensional gas chromatography (GCxGC) techniques, coupled with mass spectrometry (MS) for FA profiling and structural identification.An investigation of appropriate combinations of stationary phases was studied prior to the subsequent multidimensional analytical method development. Recently introduced ionic liquid (IL) capillary GC stationary phases covering a wide polarity range have been evaluated for fatty acid methyl esters (FAMEs) analysis in terms of their elution patterns and retention indices (e,g. ECL) on the IL columns.The performance of a newly proposed integrated GCxGC / heart-cut (H/C) MDGC-FID method for isomeric PUFA separation was then evaluated. As a practical application, abundant long chain UFA isomers with carbon numbers ranging from 18 to 22 in marine oil, as well as a dairy food product, can almost be fully separated by using GCxGC. Additionally, more than 7 other FA compounds were found in the same region by switching the system to H/C MDGC-FID under a modified GC condition. Both these applications employed fast analysis conditions.Next, GCxGC was coupled to the quadrupole-accurate mass time of flight mass spectrometry (QTOFMS). This was done to achieve accurate detection and structural confirmation of individual known or unknown branched FA obtained through predicted empirical formula and accurate mass information. The approach was applied to phospholipid fatty acids (PLFAs) from complex forest soil samples in order to investigate the microbial community. Several high abundance branched hydroxyl- (OH-), iso-/anteiso- and cyclopropyl- (cy-) FAME were clearly determined. Tentative identities of trace level OH-FAME and unusual epoxidised FAME were found according to their predicted empirical formulae and elemental compositions. Epoxidised FA were previously found in the metabolic pathway of a Gram-positive soil bacterium Bacillus megaterium. The findings indicate that this approach has great potential towards fingerprinting and FA biomarker discovery in environmental research. Lastly, comprehensive fast GCxMS with a positive chemical ionisation mode (PCI) was proposed for FA analysis in medical studies. Method performance was evaluated by application to different types of FAs including PUFAMEs and bacterial FAMEs (e.g. branched FAMEs) as well as to real biological samples (e.g. wild type and transgenic mouse brains associated with Alzheimer's disease). Methanol was selected as the most appropriate PCI chemical reagent for total FA profiling. A comparison of both rapid comprehensive GCxPCI-QMS and GCxGC approaches, for fast screening capabilities and the rapid quantitative determination of long chain PUFA isomers in mice brain associated with Alzheimer's disease, was conducted in this study. An evaluation of both methods was based on the comparison of their 2D presentations, calibration linearity and minimum levels of detection. Fast GCxMS data demonstrated the good quantification potential of this approach towards biological FA analysis, especially in medical studies.
Author: Scott Jimenez
Publisher:
ISBN: 9781536172317
Category :
Languages : en
Pages : 100
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Book Description
This book describes the methodology used to determine sterol content in coral reef food webs, and how sterol data is used in trophic ecology studies. The authors briefly explain the basics of gas chromatography and mass spectrometry as necessary tools to confidently separate, identify, and quantify sterols. Sterols from different sponge families have been described, however, few studies have been conducted involving the sterols of the freshwater sponge compared to those of the marine environment. The number of sterols present varies with the species, and this characteristic make it possible to use this data as a chemosystematic marker. In olive oil, sterols constitute the majority of the unsaponifiable fraction. In recent years, there has been increased interest in the sterols of olive oil due to their health benefits and their importance to virgin olive oil quality. Thus, the authors discuss recent findings concerning the effect of cultivars on the sterolic profile of extra virgin olive oil. The subsequent study aims to contribute to the optimisation and valorisation of virgin olive oil quality in the world olive-producing areas. This work was carried out on the study of virgin olive oil from two new olive varieties obtained through uncontrolled crossings. The sterolic fraction of argan oil is also compared to that of olive oil. The total phytosterol content ranged from 1700.80 mg/kg in chemlali oil to 150.40mg/kg in argan oil. In contrast to chemlali oil in which Î2-sitosterol is predominant, the major sterols detected in argan oil is schottenol and spinasterol. Additionally, the standards of the European Union legislation for fats and oils can help to verify olive oil authenticity using the sterolic profile. As such, this collection critiques the existing regulatory methods and standards, highlights weaknesses and proposes possible solutions to safeguard the consumer and protect the olive oil market. In closing, the authors examine the analysis of phytosterols, a multistage procedure that includes extraction, isolation/purification as a group of related compounds and a chromatographic technique for separation, identification, and quantification.
Author: Michal Holcapek
Publisher: John Wiley & Sons
ISBN: 3527836500
Category : Science
Languages : en
Pages : 897
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Book Description
Mass Spectrometry for Lipidomics All-in-one guide to successful lipidomic analysis, combining the latest advances and best practices from academia, industry, and clinical research Mass Spectrometry for Lipidomics presents a systematic overview of lipidomic analysis, covering established standards of lipid analysis, available technology, and key lipid classes, as well as applications in basic research, medicine, pharma, and the food industry. Through connecting recent technological advances with key application areas, this unique guide bridges the gap between academia and industry by translating the vast body of knowledge that has been gained in the past decade into much-needed practical advice for novices as well as routine users. Edited by the president and vice-president of the International Lipidomics Society with contributions from the top experts in lipid analysis, Mass Spectrometry for Lipidomics covers a wide range of key topics, including: Aspects of sample preparation, separation methods, different mass spectrometry modes, as well as identification and quantitation, including the use of bioinformatics tools for data analysis Identification, quantitation and profiling of lipids in different types of biological samples Analytical approaches for all major classes of biological lipids, from fatty acids to phospholipids to sterols Novel applications in biological research, clinical diagnostics, as well as food and crop science For analytical chemists, biochemists, clinical chemists, and analytical laboratories and hospitals, Mass Spectrometry for Lipidomics presents a comprehensive and authoritative overview of the subject, with unmatched expertise from practicing professionals actively involved in the latest research.
