Invertebrate Tissue Culture Methods PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Invertebrate Tissue Culture Methods PDF full book. Access full book title Invertebrate Tissue Culture Methods by Jun Mitsuhashi. Download full books in PDF and EPUB format.
Author: Jun Mitsuhashi
Publisher: Springer Science & Business Media
ISBN: 4431678751
Category : Science
Languages : en
Pages : 435
Get Book Here
Book Description
I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.
Author: Jun Mitsuhashi
Publisher: Springer Science & Business Media
ISBN: 4431678751
Category : Science
Languages : en
Pages : 435
Get Book Here
Book Description
I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.
Author: Carmel Mothersill
Publisher: Springer
ISBN:
Category : Medical
Languages : en
Pages : 446
Get Book Here
Book Description
Aquatic Invertebrate Cell Culture is a very new field which has major applications in Aquaculture, Ecotoxicology and Pathology. In essence it is realised that pathogens behave differently in host cells than the impression gained from growth on agar plates. Another major application of invertebrate cell culture is an understanding of mechanisms involved in cellular and molecular responses to environmental change. This book aims to consider all relevant advances for the development of aquatic invertebrate cell culture.
Author: Edouard Kurstak
Publisher:
ISBN:
Category : Cultures (Biology)
Languages : en
Pages : 0
Get Book Here
Book Description
Author: Paul F. Jr. Kruse
Publisher: Elsevier
ISBN: 0323142079
Category : Science
Languages : en
Pages : 897
Get Book Here
Book Description
Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.
Author: Karl Maramorosch
Publisher: CRC Press
ISBN: 9780849376429
Category : Science
Languages : en
Pages : 250
Get Book Here
Book Description
Invertebrate cell culture is increasingly being used in various areas of biological research. Research in cellular biology and pathology that previously depended primarily on in vitro investigations of vertebrate animal cell systems is now being conducted using invertebrate cells. Specialists and pioneers from the United States, Japan, Switzerland, Slovakia, and China have presented original contributions to create a well-balanced cross-section of current developments. Topics discussed include the preparation of cell culture media; cultivation of mosquito, lepidopteran, grasshopper, and tick cells; the application of such cells to mammalian and plant virus research; and diverse applications in medicine, biology, and agriculture. A special chapter devoted to the work of Japanese cell culture pioneers is also featured. All chapters are supported by tables, photographs, and up-to-date bibliographies.
Author: Karl Maramorosch
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 318
Get Book Here
Book Description
This text aims to provide readers with a balanced cross-section of current developments within the research on invertebrate cell culture. Attention is focused on such topics as: the biochemistry and physiology of cultured invertebrate cells; aspects of virus infection; novel cultivation methods; assays of viruses affecting shrimp and insect cells; engineering of invertebrate cells for the production of baculovirus pesticides; application of microgravity to in vitro cell cultivation; and other aspects of biotechnology. The large body of information brings into focus the significant recent achievements in the laboratories of Africa, America, Europe and Asia.
Author: C Vago
Publisher: Elsevier
ISBN: 0323149669
Category : Science
Languages : en
Pages : 456
Get Book Here
Book Description
Invertebrate Tissue Culture, Volume I, reviews advances in the use of tissue and organ culture in invertebrate research in physiology and pathology. It describes methods in invertebrate tissue culture, including organ culture techniques in liquid and gel media; aseptic rearing of invertebrates for tissue culture; and cell culture of organisms, ranging from Lepidoptera and Diptera to Coleoptera, Orthoptera, Dictyoptera, Hymenoptera, Hemiptera, Crustacea, Arachnida, Merostomacea, and mollusks. Organized into two sections encompassing 13 chapters, this volume begins with an overview of the general methodology in cell and organ cultures and their preparation from aseptic conditions. It then discusses methods for the examination of cultures, including those concerning ultrastructure studies by electron microscopy. The reader is also introduced to cell cultures obtained from different groups of invertebrates, with emphasis on peculiarities specific to each group; the morphology and physiology of cultured invertebrate cells; and cultivation and growth of cells. This book is a valuable resource for specialists in the field of invertebrate cell and organ culture.
Author: E. N. Willmer
Publisher: Elsevier
ISBN: 1483277232
Category : Science
Languages : en
Pages : 843
Get Book Here
Book Description
Cells and Tissues in Culture: Methods, Biology, and Physiology, Volume 3 focuses on the applications of the methods of tissue culture to various fields of investigation, including virology, immunology, and preventive medicine. The selection first offers information on molecular organization of cells and tissues in culture and tissue culture in radiobiology. Topics include cellular organization at the molecular level, fibrogenesis in tissue culture, effect of radiation on the growth of isolated cells, and irradiation of the selected parts of the cell. The publication then considers the effects of invading organisms on cells and tissues in culture and cell, tissue, and organ cultures in virus research. The book elaborates on antibody production in tissue culture and tissue culture in pharmacology. Discussions focus on early attempts at in vitro studies, tissue culture in the study of pharmacologically active agents, and methods of assessment of drug activity. The text also reviews invertebrate tissue and organ culture in cell research; introduction and methods employed in plant tissue culture; and growth, differentiation and organogenesis in plant tissue and organ cultures. The selection is a vital source of data for readers interested in the culture of cells and tissues.
Author: Jennie P. Mather
Publisher: Springer Science & Business Media
ISBN: 0585275718
Category : Science
Languages : en
Pages : 247
Get Book Here
Book Description
It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.
Author: Edouard Kurstak
Publisher: Elsevier
ISBN: 0323151469
Category : Science
Languages : en
Pages : 417
Get Book Here
Book Description
Invertebrate Tissue Culture: Applications in Medicine, Biology, and Agriculture comprises the proceedings of the IV International Conference on Invertebrate Tissue Culture, held on June 5-8, 1975 at Mont Gabriel, Quebec, Canada. The conference focuses on invertebrate organ, tissue, and cell culture, as well as cell culture limitations, pitfalls, and applications in medicine, biology, agriculture, neurophysiology, and studies of morphogenesis, differentiation, viruses, symbionts, and parasites. This reference material specifically provides information on sophisticated laboratory methods and on numerous utilizations of invertebrate cell culture techniques in medicine and biology. This book also elucidates the nutritional requirements and the establishment of cell lines. The study of viruses and protozoa of agricultural and forest importance is also shown. This book will be useful and stimulating to the readers and will provide in a single volume the results obtained in the diverse areas of research pursued by the leading exponents of invertebrate tissue culture from America, Europe, Asia, and Australia.
