Author: Jun Mitsuhashi
Publisher: CRC Press
ISBN:
Category : Science
Languages : en
Pages : 332
Book Description
A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.
Invertebrate Cell System Applications, Volume II
Author: Jun Mitsuhashi
Publisher: CRC Press
ISBN:
Category : Science
Languages : en
Pages : 332
Book Description
A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.
Publisher: CRC Press
ISBN:
Category : Science
Languages : en
Pages : 332
Book Description
A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.
Invertebrate Cell System Applications
Author: Jun Mitsuhashi
Publisher: CRC Press
ISBN: 1351090755
Category : Science
Languages : en
Pages : 417
Book Description
A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.
Publisher: CRC Press
ISBN: 1351090755
Category : Science
Languages : en
Pages : 417
Book Description
A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.
Invertebrate Cell System Applications
Author: Jun Mitsuhashi
Publisher: CRC Press
ISBN: 1351090747
Category : Science
Languages : en
Pages : 290
Book Description
A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.
Publisher: CRC Press
ISBN: 1351090747
Category : Science
Languages : en
Pages : 290
Book Description
A useful reference for those using or interested in cultured invertebrate cells, this two-volume text provides information about techniques and advances in invertebrate tissue culture. Cell lines for Insecta, Crustacea, Mollusca, and Nematoda are introduces along with their characterizations. Developments in insect biotechnology, including foreign protein production by insect cells infected with recombinant virus are described. Fundamental studies for introducing foreign genes into cultured insect cells is also presented. Wide information on studies -at cellular levels-on pathogens of insects, plants, and vertebrates is given.
Invertebrate Immune Responses
Author: Edwin L. Cooper
Publisher: Springer Science & Business Media
ISBN: 3642796931
Category : Medical
Languages : en
Pages : 224
Book Description
E. L. Cooper The Immunodefense System Because invertebrates are exceedingly diverse and numerous, estimates reveal nearly 2 million species classified in more than 20 phyla from unicellular organisms up to the complex, multicellular protostomes and deuterostomes. It is not surprising to find less diverse defense/immune responses whose effector mechanisms remain to be completely elucidated. Of course, I am not advocating that the few of us devoted to analyzing invertebrate immunity attempt the Herculean task of examining all these species to uncover some kind of unique response! As these two volumes will reveal, we are doing fairly well in examining in depth only the most miniscule examples of invertebrates, some of which have great effects on human populations such as edible crustaceans or insect pests. This is in striking contrast to the mass of information on the mammalian immune response which has been derived essentially from the mouse, a member of one phylum, Vertebrata, an approach, reductionist to be sure, but one that has served well both the technological and conceptual advances of immunology as a disci pline. The essential framework of immunology, the overwhelming burst of results since the 1960s, have emanated primarily from this single animal. We should not forget the thymus and the bird's bursa of Fabricius, without which we might have been slower to recognize the bipartite T /B system.
Publisher: Springer Science & Business Media
ISBN: 3642796931
Category : Medical
Languages : en
Pages : 224
Book Description
E. L. Cooper The Immunodefense System Because invertebrates are exceedingly diverse and numerous, estimates reveal nearly 2 million species classified in more than 20 phyla from unicellular organisms up to the complex, multicellular protostomes and deuterostomes. It is not surprising to find less diverse defense/immune responses whose effector mechanisms remain to be completely elucidated. Of course, I am not advocating that the few of us devoted to analyzing invertebrate immunity attempt the Herculean task of examining all these species to uncover some kind of unique response! As these two volumes will reveal, we are doing fairly well in examining in depth only the most miniscule examples of invertebrates, some of which have great effects on human populations such as edible crustaceans or insect pests. This is in striking contrast to the mass of information on the mammalian immune response which has been derived essentially from the mouse, a member of one phylum, Vertebrata, an approach, reductionist to be sure, but one that has served well both the technological and conceptual advances of immunology as a disci pline. The essential framework of immunology, the overwhelming burst of results since the 1960s, have emanated primarily from this single animal. We should not forget the thymus and the bird's bursa of Fabricius, without which we might have been slower to recognize the bipartite T /B system.
Invertebrate Tissue Culture Methods
Author: Jun Mitsuhashi
Publisher: Springer Science & Business Media
ISBN: 4431678751
Category : Science
Languages : en
Pages : 435
Book Description
I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.
Publisher: Springer Science & Business Media
ISBN: 4431678751
Category : Science
Languages : en
Pages : 435
Book Description
I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.
Molecular Approaches to the Study of the Ocean
Author: K.E. Cooksey
Publisher: Springer Science & Business Media
ISBN: 9401149283
Category : Science
Languages : en
Pages : 562
Book Description
Marine biological science is now studied at the molecular level and although research scientists depend on information gained using molecular techniques, there is no book explaining the philosophy of this approach. Molecular Approaches to the Study of the Ocean introduces the reasons why molecular technology is such a powerful tool in the study of the oceans, describing the types of techniques that can be used, why they are useful and gives examples of their application. Molecular biological techniques allow phylogenetic relationships to be explored in a manner that no macroscopic method can; although the book deals with organisms near the base of the marine food web, the ideas can be used in studies of macroorganisms as well as those in freshwater environments.
Publisher: Springer Science & Business Media
ISBN: 9401149283
Category : Science
Languages : en
Pages : 562
Book Description
Marine biological science is now studied at the molecular level and although research scientists depend on information gained using molecular techniques, there is no book explaining the philosophy of this approach. Molecular Approaches to the Study of the Ocean introduces the reasons why molecular technology is such a powerful tool in the study of the oceans, describing the types of techniques that can be used, why they are useful and gives examples of their application. Molecular biological techniques allow phylogenetic relationships to be explored in a manner that no macroscopic method can; although the book deals with organisms near the base of the marine food web, the ideas can be used in studies of macroorganisms as well as those in freshwater environments.
Insect Cell Cultures
Author: Just M. Vlak
Publisher: Springer Science & Business Media
ISBN: 0306468506
Category : Science
Languages : en
Pages : 303
Book Description
A comprehensive reference work covering the key issues in insect cell cultures, this text includes 30 review papers on such topics as: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; and economics and regulatory aspects.; This text should be useful for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.
Publisher: Springer Science & Business Media
ISBN: 0306468506
Category : Science
Languages : en
Pages : 303
Book Description
A comprehensive reference work covering the key issues in insect cell cultures, this text includes 30 review papers on such topics as: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; and economics and regulatory aspects.; This text should be useful for cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.
Exploitation of Microorganisms
Author: D.G. Jones
Publisher: Springer Science & Business Media
ISBN: 940111532X
Category : Science
Languages : en
Pages : 494
Book Description
Microbiology may be described as one of the younger sciences with its history, as a precise subject, only dating as far back as Pasteur in the mid 1800s and his revelation both of the role of microorganisms in nature and their importance to human welfare. Medical scientists rapidly took up the challenge, with their area of microbiology flourishing and expanding almost in complete isolation from the rest of biology. We now know, of course, that microorganisms have always played an important, if not essential role, in the biosphere with fermented foods and beverages, plant and animal diseases and nutrient cycling foremost in their sphere of activities. Within the last twenty years, microbiology has received two enormous boosts with the developments in microbial genetics and genetic engineering probably being the most influential, and the greater awareness of pollution and environmental sustainability following a close second. In 1990, your editor had the privilege and pleasure of being elected as President of The Association of Applied Biologists in the United King dom and, as the topic for his three-day Presidential Conference, chose 'The exploitation of microorganisms in applied biology'. This meeting stimu lated great interest in a wide range of subject areas, from weed control to nematology, from plant breeding to plant pathology, from mushrooms to mycorrhiza. The proceedings of this meeting were published in Aspects of Applied Biology, No. 24, 1990.
Publisher: Springer Science & Business Media
ISBN: 940111532X
Category : Science
Languages : en
Pages : 494
Book Description
Microbiology may be described as one of the younger sciences with its history, as a precise subject, only dating as far back as Pasteur in the mid 1800s and his revelation both of the role of microorganisms in nature and their importance to human welfare. Medical scientists rapidly took up the challenge, with their area of microbiology flourishing and expanding almost in complete isolation from the rest of biology. We now know, of course, that microorganisms have always played an important, if not essential role, in the biosphere with fermented foods and beverages, plant and animal diseases and nutrient cycling foremost in their sphere of activities. Within the last twenty years, microbiology has received two enormous boosts with the developments in microbial genetics and genetic engineering probably being the most influential, and the greater awareness of pollution and environmental sustainability following a close second. In 1990, your editor had the privilege and pleasure of being elected as President of The Association of Applied Biologists in the United King dom and, as the topic for his three-day Presidential Conference, chose 'The exploitation of microorganisms in applied biology'. This meeting stimu lated great interest in a wide range of subject areas, from weed control to nematology, from plant breeding to plant pathology, from mushrooms to mycorrhiza. The proceedings of this meeting were published in Aspects of Applied Biology, No. 24, 1990.
Animal Cell Technology: From Target to Market
Author: European Society of Animal Cell Technology. General Meeting
Publisher: Springer Science & Business Media
ISBN: 9781402002649
Category : Medical
Languages : en
Pages : 684
Book Description
This book is the lasting product, a resource of up-to-date information in the scientific literature for the field of animal cell technology, as it was presented during a pleasant and stimulating meeting in Tylösand, Sweden, in June 2001. The title of the meeting, From Target to Market, indicates the usefulness of Animal Cell Technology during all steps in the pharmaceutical development process. Following the biotech products reaching the market, it shows an upward trend in the contribution of biotech products to total New Molecular Entity output in the nineties, which continued until 1996 when biotech represented 25% of the annual output. Since then the proportion has been decreasing. A perceived hurdle from a market perspective is that a protein per definition is biodegradable and thus requires intravenous, or for some drugs subcutaneous administration. New promising administration technologies such as pulmonary delivery were highlighted at this meeting. The emphasis on project selection prior to entry in the development phase has triggered a portfolio management using more extensive preclinical data before a development decision is taken. Animal cells have become a very important tool in the drug discovery process. The next generation of products will evolve from applications such as gene therapy, novel vaccines, cell therapy, and gene regulation. Animal cell technology has a major role to play in the post-sequence era.
Publisher: Springer Science & Business Media
ISBN: 9781402002649
Category : Medical
Languages : en
Pages : 684
Book Description
This book is the lasting product, a resource of up-to-date information in the scientific literature for the field of animal cell technology, as it was presented during a pleasant and stimulating meeting in Tylösand, Sweden, in June 2001. The title of the meeting, From Target to Market, indicates the usefulness of Animal Cell Technology during all steps in the pharmaceutical development process. Following the biotech products reaching the market, it shows an upward trend in the contribution of biotech products to total New Molecular Entity output in the nineties, which continued until 1996 when biotech represented 25% of the annual output. Since then the proportion has been decreasing. A perceived hurdle from a market perspective is that a protein per definition is biodegradable and thus requires intravenous, or for some drugs subcutaneous administration. New promising administration technologies such as pulmonary delivery were highlighted at this meeting. The emphasis on project selection prior to entry in the development phase has triggered a portfolio management using more extensive preclinical data before a development decision is taken. Animal cells have become a very important tool in the drug discovery process. The next generation of products will evolve from applications such as gene therapy, novel vaccines, cell therapy, and gene regulation. Animal cell technology has a major role to play in the post-sequence era.
The Molecular Biology of Insect Disease Vectors
Author: J.M. Crampton
Publisher: Springer Science & Business Media
ISBN: 9400915357
Category : Science
Languages : en
Pages : 590
Book Description
Only one generation ago, entomology was a proudly isolated discipline. In Comstock Hall, the building of the Department of Entomology at Cornell University where I was first introduced to experimental science in the laboratory of Tom Eisner, those of us interested in the chemistry of life felt like interlopers. In the 35 years that have elapsed since then, all of biology has changed, and entomology with it. Arrogant molecular biologists and resentful classical biologists might think that what has happened is a hostile take-over of biology by molecular biology. But they are wrong. More and more we now understand that the events were happier and much more exciting, amounting to a new synthesis. Molecular Biology, which was initially focused on the simplest of organisms, bacteria and viruses, broke out of its confines after the initial fundamental questions were answered - the structure of DNA, the genetic code, the nature of regulatory genes - and, importantly, as its methods became more and more generally applicable. The recombinant DNA revo lution of the 1970s, the development of techniques for sequencing macromolecules, the polymerase chain reaction, new molecular methods of genetic analysis, all brought molecular biology face to face with the infinite complexity and the exuber ant diversity of life. Molecular biology itself stopped being an isolated diScipline, pre occupied with the universal laws of life, and became an approach to addressing fas cinating specific problems from every field of biology.
Publisher: Springer Science & Business Media
ISBN: 9400915357
Category : Science
Languages : en
Pages : 590
Book Description
Only one generation ago, entomology was a proudly isolated discipline. In Comstock Hall, the building of the Department of Entomology at Cornell University where I was first introduced to experimental science in the laboratory of Tom Eisner, those of us interested in the chemistry of life felt like interlopers. In the 35 years that have elapsed since then, all of biology has changed, and entomology with it. Arrogant molecular biologists and resentful classical biologists might think that what has happened is a hostile take-over of biology by molecular biology. But they are wrong. More and more we now understand that the events were happier and much more exciting, amounting to a new synthesis. Molecular Biology, which was initially focused on the simplest of organisms, bacteria and viruses, broke out of its confines after the initial fundamental questions were answered - the structure of DNA, the genetic code, the nature of regulatory genes - and, importantly, as its methods became more and more generally applicable. The recombinant DNA revo lution of the 1970s, the development of techniques for sequencing macromolecules, the polymerase chain reaction, new molecular methods of genetic analysis, all brought molecular biology face to face with the infinite complexity and the exuber ant diversity of life. Molecular biology itself stopped being an isolated diScipline, pre occupied with the universal laws of life, and became an approach to addressing fas cinating specific problems from every field of biology.