Interaction of 16S and 23S Ribosomal RNA with Functional Ligands, Implications for the Mechanism of Protein Synthesis PDF Download
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Author: Danesh Moazed
Publisher:
ISBN:
Category : Ligands
Languages : en
Pages : 394
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Book Description
Author: Danesh Moazed
Publisher:
ISBN:
Category : Ligands
Languages : en
Pages : 394
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Book Description
Author: Robert Alexander Baan
Publisher:
ISBN:
Category : Peptides
Languages : en
Pages : 120
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Book Description
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 816
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Book Description
Author: Peining Dong
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 350
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Book Description
Author: Lihong Jiang
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 172
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Book Description
Author: Fazilah Abdul-Latif
Publisher:
ISBN:
Category : Proteins
Languages : en
Pages : 198
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Book Description
Ribosomes are ribonucleoprotein particles that are essential for the process of protein synthesis in all living systems. Escherichia coli, eubacterial organisms, have ribosomes which display a 70S sedimentation coefficient. They are formed by the association of two ribonucleoprotein subunits which sediment at 50S and 30S. The 30S subunit is a construct made of 21 different proteins and 16S ribosomal RNA. The 3'-terminus of this rRNA was the focus of the research recounted in this dissertation. This tract of 10 nucleotides in rRNA is absolutely conserved in eubacterial organisms and is asserted to be crucial for proper initiation of protein synthesis in eubacteria. Here I report the results of experiments done with 16S rRNA missing a part of the conserved 3'-terminus. These experiments were undertaken to provide a refinement of our understanding of the requirement for and function of this conserved segment of 16S rRNA. The deletions were made directly within mature 16S rRNA using RNase H and a 10 nucleotide synthetic DNA complementary to the 3'-terminus of 16S rRNA. The synthetic DNA was hybridized to 16S rRNA and then treated with RNase H. RNase H will only attack RNA when it is basepaired with DNA. This permits site-directed mutagenisis on the mature RNA. This deletion strategy efficiently yielded a 3'-terminal nucleotide deletion in E. coli 16S rRNA. This 3'-terminal deletion did not impair in vitro 30S subunit assembly. Therefore, the conservation of the sequence is not necessary for ribosome assembly. To investigate the functional properties of the modified particles an in vitro protein translation system primed with a natural mRNA was employed. The mRNA was viral MS2 messenger. Modified particles did translate the MS2 mRNA but the fidelity with which the translation occurred was diminished.
Author: Astrid Sigel
Publisher: Springer
ISBN: 3319217569
Category : Science
Languages : en
Pages : 663
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Book Description
MILS-16 provides an up-to-date review of the impact of alkali metal ions on life. Their bioinorganic chemistry and analytical determination, the solid state structures of bio-ligand complexes and the properties of alkali metal ions in solution in the context of all kinds of biologically relevant ligands are covered, this includes proteins (enzymes) and nucleic acids (G-quadruplexes). Minerals containing sodium (Na+) and potassium (K+) are abundant in the Earth's crust, making Na+ and K+ easily available. In contrast, the alkali elements lithium (Li+), rubidium, and cesium are rare and the radioactive francium occurs only in traces. Since the intra- and extracellular, as well as the compartmental concentrations of Na+ and K+ differ significantly, homeostasis and active transport of these ions are important; this involves transporters/carriers and pore-forming ion channel proteins. Systems like Na+/K+-ATPases, H+/K+-ATPases or Na+/H+ antiporters are thoroughly discussed. The role of K+ in photosynthesis and the role of Na+ in charging the "battery of life" are pointed out. Also, the relationships between alkali metal ions and diseases (e.g., Parkinson or traumatic brain injury) are covered and the relevance of Li+ salts in medicine (pharmacology and mechanism) is reviewed. This and more is treated in an authoritative and timely manner in the 16 stimulating chapters of Volume 16, The Alkali Metal Ions: Their Role for Life, which are written by 44 internationally recognized experts from 12 nations. The impact of this vibrant research area is manifested in nearly 3000 references, over 30 tables and more than 150 illustrations (two thirds in color). MILS-16 also provides excellent information for teaching. Astrid Sigel, Helmut Sigel, and Roland K. O. Sigel have long-standing interests in Biological Inorganic Chemistry. Their research focuses on metal ion interactions with nucleotides and nucleic acids and on related topics. They edited previously 44 volumes in the series Metal Ions in Biological Systems.
Author: Aleksandr S. Spirin
Publisher: Springer Science & Business Media
ISBN: 3642884466
Category : Science
Languages : en
Pages : 173
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Book Description
This monograph is neither a historical outline of the development of the concepts of protein biosynthesis and the structure and functions of the ribosomes, nor an exhaustive survey of the literature on these questions. The monograph is based upon an analysis of the modern trends in this field. The purpose of the monograph was to formulate more or less generalized representations of the structure and" function of the ribosome, as we envision it at the present day. It may be that this attempt is premature for a number of reasons, and the concepts outlined here will very soon be revised. Nonetheless, despite this risk, we believe it to be advisable to undertake this attempt for the following reasons: firstly, the undertaken analysis could aid in the comprehension of the substantial mass of extremely scattered experimental data on the ribosomes presently available; secondly, in any event, even if most of the concepts outlined rapidly become obselete, they can still serve as a stimulus for a whole series of experiments; and thirdly, we hope that some of the concepts outlined will still remain essentially correct and relatively stable. In view of the aforementioned, we should make the following reservations. First of all, we made no attempt to cite all the literature on the problems discussed, but considered it sufficient to illustrate the various premises with one or several sample references.
Author: Martin Alexander
Publisher: Academic Press
ISBN: 0080548482
Category : Science
Languages : en
Pages : 963
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Book Description
The First Edition of the Encyclopedia of Microbiology was hailed by leading scientists and researchers around the world as "excellent," "outstanding," and "impressive." This Second Edition will serve as an up-to-date version of this reference which has been useful to academic, industrial, and personal libraries for years. The Encyclopedia of Microbiology, Second Edition both challenges and stimulates the reader, and illustrates the importance of microbiology, a field that cannot be over emphasized in this booming biotechnology age. Key Features * Completely redesigned and revised approach with 65% new material * Contains approximately 300 articles, 1000 illustrations, and 400 tables * New design includes thematic table of contents, combined glossary of terms, and appendix * Provides color plate sections in each volume * 17 subject areas, including exciting coverage of microbes in extreme environments and microbes in emerging infections
Author: Marina V. Rodnina
Publisher: Springer Science & Business Media
ISBN: 3709102154
Category : Medical
Languages : en
Pages : 428
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Book Description
The ribosome is a macromolecular machine that synthesizes proteins with a high degree of speed and accuracy. Our present understanding of its structure, function and dynamics is the result of six decades of research. This book collects over 40 articles based on the talks presented at the 2010 Ribosome Meeting, held in Orvieto, Italy, covering all facets of the structure and function of the ribosome. New high-resolution crystal structures of functional ribosome complexes and cryo-EM structures of translating ribosomes are presented, while partial reactions of translation are examined in structural and mechanistic detail, featuring translocation as a most dynamic process. Mechanisms of initiation, both in bacterial and eukaryotic systems, translation termination, and novel details of the functions of the respective factors are described. Structure and interactions of the nascent peptide within, and emerging from, the ribosomal peptide exit tunnel are addressed in several articles. Structural and single-molecule studies reveal a picture of the ribosome exhibiting the energy landscape of a processive Brownian machine. The collection provides up-to-date reviews which will serve as a source of essential information for years to come.
