Integrating High-throughput Phenotyping, Genomic Selection, and Spatial Analysis for Plant Breeding and Management

Integrating High-throughput Phenotyping, Genomic Selection, and Spatial Analysis for Plant Breeding and Management PDF Author: Margaret Rose Krause
Publisher:
ISBN:
Category :
Languages : en
Pages : 220

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Book Description
Recent advances in high-throughput phenotyping, genomics, and precision agriculture have provided plant breeders and farmers with a wealth of information on the growth and development of crop plants. Methods for effectively leveraging these data resources are needed in order to drive genetic gain in breeding programs and to increase efficiency in farming systems. Three novel approaches for the development and management of high yielding, adapted crop varieties are presented. First, aerial hyperspectral reflectance phenotypes of bread wheat (Triticum aestivum L.) were used to develop relationship matrices for the prediction of grain yield within and across environments with genomic selection. Multi-kernel models combining marker/pedigree information with hyperspectral reflectance phenotypes gave the highest accuracies overall; however, improvements in accuracy over single-kernel marker- and pedigree-based models were reduced when correcting for days to heading. Second, aerial phenotypes collected on small, unreplicated plots representing the seed limited stage of wheat breeding programs were evaluated for their potential use as selection criteria for improving grain yield. The aerial phenotypes were shown to be heritable and positively correlated with grain yield measurements evaluated in replicated yield trials. Results also suggest that selection on aerial phenotypes at the seed-limited stage would cause a directional response in phenology due to confounding of those traits. Lastly, on-farm trials were conducted in collaboration with the New York Corn and Soybean Growers Association to identify optimal planting rates for corn (Zea mays L.) and soybean (Glycine max L.) given the underlying spatial variability of the soil and topographical characteristics of the fields. A random forest regression-based approach was created to develop variable rate planting designs for maximizing yields.

Integrating High-throughput Phenotyping, Genomic Selection, and Spatial Analysis for Plant Breeding and Management

Integrating High-throughput Phenotyping, Genomic Selection, and Spatial Analysis for Plant Breeding and Management PDF Author: Margaret Rose Krause
Publisher:
ISBN:
Category :
Languages : en
Pages : 220

Get Book Here

Book Description
Recent advances in high-throughput phenotyping, genomics, and precision agriculture have provided plant breeders and farmers with a wealth of information on the growth and development of crop plants. Methods for effectively leveraging these data resources are needed in order to drive genetic gain in breeding programs and to increase efficiency in farming systems. Three novel approaches for the development and management of high yielding, adapted crop varieties are presented. First, aerial hyperspectral reflectance phenotypes of bread wheat (Triticum aestivum L.) were used to develop relationship matrices for the prediction of grain yield within and across environments with genomic selection. Multi-kernel models combining marker/pedigree information with hyperspectral reflectance phenotypes gave the highest accuracies overall; however, improvements in accuracy over single-kernel marker- and pedigree-based models were reduced when correcting for days to heading. Second, aerial phenotypes collected on small, unreplicated plots representing the seed limited stage of wheat breeding programs were evaluated for their potential use as selection criteria for improving grain yield. The aerial phenotypes were shown to be heritable and positively correlated with grain yield measurements evaluated in replicated yield trials. Results also suggest that selection on aerial phenotypes at the seed-limited stage would cause a directional response in phenology due to confounding of those traits. Lastly, on-farm trials were conducted in collaboration with the New York Corn and Soybean Growers Association to identify optimal planting rates for corn (Zea mays L.) and soybean (Glycine max L.) given the underlying spatial variability of the soil and topographical characteristics of the fields. A random forest regression-based approach was created to develop variable rate planting designs for maximizing yields.

High-Throughput Crop Phenotyping

High-Throughput Crop Phenotyping PDF Author: Jianfeng Zhou
Publisher: Springer Nature
ISBN: 3030737349
Category : Science
Languages : en
Pages : 249

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Book Description
This book provides an overview of the innovations in crop phenotyping using emerging technologies, i.e., high-throughput crop phenotyping technology, including its concept, importance, breakthrough and applications in different crops and environments. Emerging technologies in sensing, machine vision and high-performance computing are changing the world beyond our imagination. They are also becoming the most powerful driver of the innovation in agriculture technology, including crop breeding, genetics and management. It includes the state of the art of technologies in high-throughput phenotyping, including advanced sensors, automation systems, ground-based or aerial robotic systems. It also discusses the emerging technologies of big data processing and analytics, such as advanced machine learning and deep learning technologies based on high-performance computing infrastructure. The applications cover different organ levels (root, shoot and seed) of different crops (grains, soybean, maize, potato) at different growth environments (open field and controlled environments). With the contribution of more than 20 world-leading researchers in high-throughput crop phenotyping, the authors hope this book provides readers the needed information to understand the concept, gain the insides and create the innovation of high-throughput phenotyping technology.

Molecular Plant Breeding

Molecular Plant Breeding PDF Author: Yunbi Xu
Publisher: CABI
ISBN: 1845936248
Category : Science
Languages : en
Pages : 756

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Book Description
Recent advances in plant genomics and molecular biology have revolutionized our understanding of plant genetics, providing new opportunities for more efficient and controllable plant breeding. Successful techniques require a solid understanding of the underlying molecular biology as well as experience in applied plant breeding. Bridging the gap between developments in biotechnology and its applications in plant improvement, Molecular Plant Breeding provides an integrative overview of issues from basic theories to their applications to crop improvement including molecular marker technology, gene mapping, genetic transformation, quantitative genetics, and breeding methodology.

Introducing Sparsity Into Selection Index Methodology with Applications to High-throughput Phenotyping and Genomic Prediction

Introducing Sparsity Into Selection Index Methodology with Applications to High-throughput Phenotyping and Genomic Prediction PDF Author: Marco Antonio Lopez Cruz
Publisher:
ISBN:
Category : Electronic dissertations
Languages : en
Pages : 149

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Book Description
Research in plant and animal breeding has been largely focused on the development of methods for a more efficient selection by altering the factors that affect genetic progress: selection intensity, selection accuracy, genetic variance, and length of the breeding cycle. Most of the breeding efforts have been primarily towards increasing selection accuracy and reducing the breeding cycle.Genomic selection has been successfully adopted by many public and private breeding organizations. Over years, these institutions have developed and accumulated large volumes of genomic data linked to phenotypes from multiple populations and multiple generations. This data abundance offers the opportunity to revolutionize genetic research. However, these data sets are also increasingly heterogeneous, with many subpopulations and multiple generations represented in the data. This translates into potentially heterogeneous allele frequencies and different LD patterns, thus leading to SNP-effect heterogeneity.Genomic selection methods were developed with reference to homogeneous populations in which SNP-effects are assumed constant across the whole population. These methods are not necessarily optimal for the contemporary available data sets for model training. Therefore, a first focus of this dissertation is on developing novel methods that can leverage the large-scale of modern data sets while coping with the heterogeneity and complexity of this type of data.In recent years, there have also been important advances in high-throughput phenotyping (HTP) technologies that can generate large volumes of data at multiple time-points of a crop. Examples of this include hyper-spectral imaging technologies that can capture the reflectance of electromagnetic power by crops at potentially thousands of wavelengths. The integration of HTP in genetic evaluations represents a great opportunity to further advance plant breeding; however, the high-dimensional nature of HTP data poses important challenges. Therefore, a second focus of this dissertation is on the development of a novel approach to efficiently incorporate HTP data for breeding values prediction.Thus, this dissertation aims to contribute novel methods that can improve the accuracy of genomic prediction by optimizing the use of large, potentially heterogeneous, genomic data sets and by enabling the integration of HTP data. We present a novel statistical approach that combines the standard selection index methodology with variable-selection methods commonly used in machine learning and statistics, and developed software to implement the method. Our approach offers solutions to both genomic selection with potentially highly heterogeneous genomic data sets, and the integration of HTP in genetic evaluations.

Stability of Traits Across Environments Using Image Phenotyping and Genotyping

Stability of Traits Across Environments Using Image Phenotyping and Genotyping PDF Author: Nicolas Morales
Publisher:
ISBN:
Category :
Languages : en
Pages : 0

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Book Description
Genetic gain for important agronomic traits can be accelerated in plant breeding by better understanding the environmental effects on field experimental plots over the growing season. Today, aerial image remote sensing via unoccupied aerial vehicles (UAVs) offers cost-effective collection of high throughput phenotypes (HTPs) with high temporal and spatial resolution. However, utilizing HTP to evaluate the genetic merit of tested accessions in a modern breeding program requires effective integration of the captured imagery with genome-wide marker data, experimental design and geographic information, agronomic phenotypic data, and soil and weather data. Furthermore, plant breeders require timely predictions of genetic effects for selecting accessions to advance; therefore, the data should be readily integrated into a quantitative genetics statistical framework. Hence, this dissertation presents four chapters: (1) a database schema for storing genome-wide marker data, (2) a web-database platform for managing plant breeding programs and their experiments, (3) a web-database tool for reliably processing aerial imagery into HTP, and (4) a statistical approach integrating HTP with genomic data to better resolve genetic effects over spatio-temporal environmental effects. In (1), a NoSQL data model is presented within the Chado database schema, utilizing the NoSQL and relational capabilities of PostgreSQL to link the genome-wide marker data and the plant breeding experimental data, respectively. Benchmarking demonstrates computation of a genomic relationship matrix (GRM) and a genome wide association study (GWAS) for datasets involving 1,325 diploid Zea mays L. (maize), 314 triploid Musa acuminata (banana), and 924 diploid Manihot esculenta (cassava) samples genotyped with 955,690, 142,119, and 287,952 genotype-by-sequencing (GBS) markers, respectively. In (2), Breedbase illustrates a web-database platform enabling plant breeders around the world to manage their breeding program data in a standardized process. Importantly, the Breeding API (BrAPI) allows open access and interoperability to the data. Then (3) focuses on ImageBreed as a web-database tool for processing aerial image phenotypes into HTP. Multi-spectral or color imagery from UAVs or from fixed camera systems can be uploaded, processed into orthophotomosaics if required, designated into geospatially referenced plot-polygons, and then summarized into vegetation indices (VIs) or convolutional neural network (CNN) HTP. In (4), the normalized difference vegetation index (NDVI) collected on several years of Genomes-to-Fields (G2F) hybrid maize (Zea mays L.) field experiments is used to improve genomic prediction for grain yield, grain moisture, and ear height. The proposed approach enables greater understanding of spatial heterogeneity in the field and improves the estimation of genetic effects. To conclude, continued aggregation of genomic and image data, coupled with statistical approaches, will enable plant breeders to better understand the stability of genetic effects across space and time. Future research into latent genetic spaces embedded in ground rover lidar point clouds and aerial imagery is an exciting avenue to understanding the permanent environment and genetic stability of accessions.

Genetic Data Analysis for Plant and Animal Breeding

Genetic Data Analysis for Plant and Animal Breeding PDF Author: Fikret Isik
Publisher: Springer
ISBN: 3319551779
Category : Science
Languages : en
Pages : 409

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Book Description
This book fills the gap between textbooks of quantitative genetic theory, and software manuals that provide details on analytical methods but little context or perspective on which methods may be most appropriate for a particular application. Accordingly this book is composed of two sections. The first section (Chapters 1 to 8) covers topics of classical phenotypic data analysis for prediction of breeding values in animal and plant breeding programs. In the second section (Chapters 9 to 13) we provide the concept and overall review of available tools for using DNA markers for predictions of genetic merits in breeding populations. With advances in DNA sequencing technologies, genomic data, especially single nucleotide polymorphism (SNP) markers, have become available for animal and plant breeding programs in recent years. Analysis of DNA markers for prediction of genetic merit is a relatively new and active research area. The algorithms and software to implement these algorithms are changing rapidly. This section represents state-of-the-art knowledge on the tools and technologies available for genetic analysis of plants and animals. However, readers should be aware that the methods or statistical packages covered here may not be available or they might be out of date in a few years. Ultimately the book is intended for professional breeders interested in utilizing these tools and approaches in their breeding programs. Lastly, we anticipate the usage of this volume for advanced level graduate courses in agricultural and breeding courses.

High-Throughput Plant Phenotyping

High-Throughput Plant Phenotyping PDF Author: Argelia Lorence
Publisher: Springer Nature
ISBN: 1071625373
Category : Science
Languages : en
Pages : 300

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Book Description
This volume looks at a collection of the latest techniques used to quantify the genome-by-environment-by-management (GxExM) interactions in a variety of model and plant crops. The chapters in this book are organized into five parts. Part One discusses high-throughput plant phenotyping (HTPP) protocols for plants growing under controlled conditions. Part Two present novel algorithms for extracting data from seed images, color analysis from fruits, and other digital readouts from 2D objects. Part Three covers molecular imaging protocols using PET and X-ray approaches, and Part Four presents a collection of HTPP techniques for crops growing under field conditions. The last part focuses on molecular analysis, metabolomics, network analysis, and statistical methods for the quantitative genetic analysis of HTP data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, High-Throughput Plant Phenotyping: Review and Protocols is a valuable resource for both novice and expert researchers looking to learn more about this important field. Chapter 21 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Advances and Applications of Cost-Effective, High-Throughput Genotyping Technologies for Sustainable Agriculture

Advances and Applications of Cost-Effective, High-Throughput Genotyping Technologies for Sustainable Agriculture PDF Author: Nisha Singh
Publisher: Frontiers Media SA
ISBN: 2832541860
Category : Science
Languages : en
Pages : 196

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Book Description
Recent advances in next-generation sequencing-driven mass production of genomic data and various other integrated techniques have considerably broadened and deepened our understanding of living organisms' molecular systems. Because complex quantitative traits are difficult to select due to low heritability, conventional plant breeding relies on phenotypic selection and breeder experience, it takes longer to develop a new, improved variety. For association studies to identify DNA markers linked to these complex traits, genotyping chip arrays allow genotyping of thousands of markers in a short amount of time. Plant breeding consistency and predictability have improved thanks to advances in genomics. NGS technologies bring new tools and concepts that can enhance the precision and efficiency of plant breeding such as cost-effective, high throughput genotyping technologies for sustainable agriculture. These genotyping technologies will be lowering the time and cost of developing high-quality food crops that are stress-resistant while still having a high nutritional value. This Research Topic focuses on recent advancements in NGS-related technologies, mainly the development of cost-effective high-throughput genotyping platforms with a wide range of bioinformatics tools, and possible translational multi-omics applications in crop breeding programs for sustainable agriculture.

Phenomics

Phenomics PDF Author: Roberto Fritsche-Neto
Publisher: Springer
ISBN: 3319136771
Category : Science
Languages : en
Pages : 145

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Book Description
This book represents a pioneer initiative to describe the new technologies available for next-generation phenotyping and applied to plant breeding. Over the last several years plant breeding has experienced a true revolution. Phenomics, i.e., high-throughput phenotyping using automation, robotics and remote data collection, is changing the way cultivars are developed. Written in an easy to understand style, this book offers an indispensable reference work for all students, instructors and scientists who are interested in the latest innovative technologies applied to plant breeding.

Smart Plant Breeding for Field Crops in Post-genomics Era

Smart Plant Breeding for Field Crops in Post-genomics Era PDF Author: Devender Sharma
Publisher: Springer Nature
ISBN: 981198218X
Category : Science
Languages : en
Pages : 424

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Book Description
This book emphasizes on cutting-edge next-generation smart plant breeding approaches for maximizing the use of genomic resources generated by high-throughput genomics in the post-genomic era. Through this book the readers would learn about the recent development in the genomic approaches such as genotype by sequencing (GBS) for genomic analysis (SNPs, Single Nucleotide Polymorphism), whole-genome re-sequencing (WGRS) and RNAseq for transcriptomic analysis (DEGs, Differentially Expressed Genes). To maximize the genetic gains in the cereal/food crops, the book covers topics on transgenic breeding, genome editing, high-throughput phenotyping, reliable/precision phenotyping and genomic information-based analysis. In the era of climate change and the ever-increasing population, food security and nutritional security are the primary concern of plant breeders, growers, and policymakers to address the UN’s sustainable development goals. Chapters of this book cohere around these goals and covers techniques such as (QTL mapping, association studies, candidate gene identification), omics, RNAi [through micro RNA (miRNA), small interfering RNA (siRNA) and artificial micro RNA (amiRNA)]. It also covers other genomic techniques like antisense technology, genome editing (CRISPR/cas9, base editing) and epigenomics that assist the crop improvement programmes to fulfil the UNs sustainable development goals. It explores the influence of rapidly available sequencing data assisting in the next generation breeding programmes. This volume is a productive resource for the students, researchers, scientists, teachers, public and private sector stakeholders involved in the genetic enhancement of cereal crops.