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Author: Xiaodong Wang
Publisher: Frontiers Media SA
ISBN: 2832534031
Category : Science
Languages : en
Pages : 114
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Book Description
Author: Xiaodong Wang
Publisher: Frontiers Media SA
ISBN: 2832534031
Category : Science
Languages : en
Pages : 114
Get Book Here
Book Description
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 18
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Book Description
In our short review provides perspective regarding the use of mass spectrometry imaging (MSI) to study the rhizosphere. It also serves to complement the multi-omic-focused review by White et al. in this journals' issue. MSI is capable of elucidating chemical distributions within samples of interest in situ, and thus can provide spatial context to MS omics data in complementary experimental endeavors. Most MSI-based studies of plant-microbe interactions have focused on the phyllosphere and on the "associated rhizosphere" (our term for material that is not removed during harvesting). Sample preparation for these in situ analyses tends to be a limiting factor. Our studies, however, have provided valuable insights into the spatial arrangement of proteins, peptides, lipids, and other metabolites within these systems. We intend this short review to be a primer on the fundamentals of MSI and its role in plant-microbe analysis. Finally, we offer a perspective on the future of MSI and its use in understanding the molecular transformations beyond what we call the associated rhizosphere, one which extends to the rest of rhizosphere and into the bulk soil.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 8
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Book Description
Direct visualization of plant tissues by matrix assisted laser desorption ionization-mass spectrometry imaging (MALDI-MSI) has revealed key insights into the localization of metabolites in situ. Recent efforts have determined the spatial distribution of primary and secondary metabolites in plant tissues and cells. Strategies have been applied in many areas of metabolism including isotope flux analyses, plant interactions, and transcriptional regulation of metabolite accumulation. Technological advances have pushed achievable spatial resolution to subcellular levels and increased instrument sensitivity by several orders of magnitude. Furthermore, it is anticipated that MALDI-MSI and other MSI approaches will bring a new level of understanding to metabolomics as scientists will be encouraged to consider spatial heterogeneity of metabolites in descriptions of metabolic pathway regulation.
Author: Bindesh Shrestha
Publisher: Elsevier
ISBN: 0128189991
Category : Science
Languages : en
Pages : 272
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Book Description
Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules. The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions. - Provides the introductory foundations of imaging mass spectrometry for those new to the technique - Organized by topic to facilitate a quick deep dive, allowing researchers to immediately apply the imaging MS techniques to their work - Includes case studies summarizing the imaging MS techniques developed for the class of molecules
Author: Tiffany Siegel Porta
Publisher: Royal Society of Chemistry
ISBN: 1839162414
Category : Science
Languages : en
Pages : 541
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Book Description
This book gathers knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging for postgraduate and professional researchers in academia and in industry where it has direct application to clinical research.
Author: Lin He
Publisher: Humana
ISBN: 9781493954087
Category : Medical
Languages : en
Pages : 0
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Book Description
This volume presents new developments in the field of mass spectrometry imaging, covering imaging, software, data analysis, new instrumentations, and new methodological approaches. Chapters provide detailed operational instructions from sample preparation to method selection, from comparative quantification to structural identification and from data collection to visualization of small molecule mapping in complex samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mass Spectrometry Imaging of Small Molecules aims to to bring the rapidly maturing methods of metabolic imaging to life science researchers and to minimize technical intimidation in adapting new technological platforms in biological research.
Author: Ramesh Namdeo Pudake
Publisher: Springer Nature
ISBN: 981160889X
Category : Science
Languages : en
Pages : 279
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Book Description
This book presents a timely review of the latest advances in rhizosphere biology, which have been facilitated by the application of omics tools. It includes chapters on the use of various omics tools in rhizosphere biology, focusing on understanding plant and soil microbe interactions. The role of proteomics and metagenomics in research on symbiotic association is also discussed in detail. The book also includes chapters on the use of omics tools for the isolation of functional biomolecules from rhizospheric microorganisms. The book’s respective sections describe and provide detailed information on important omics tools, such as genomics, transcriptomics, proteomics, metabolomics and meta-epigenomics. In turn, the book promotes and describes the combined use of plant biology, microbial ecology, and soil sciences to design new research strategies and innovative methods in soil biology. Lastly, it highlights the considerable potential of the rhizosphere in terms of crop productivity, bioremediation, ecological engineering, plant nutrition and health, as well as plant adaptation to stress conditions. This book offers both a practical guide and reference source for all scientists working in soil biology, plant pathology, etc. It will also benefit students studying soil microbiology, and researchers studying rhizosphere structure.
Author: Liang Chen
Publisher: Springer Nature
ISBN: 9819706807
Category :
Languages : en
Pages : 509
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Book Description
Author: Kashif Ali
Publisher: Frontiers Media SA
ISBN: 2832539785
Category : Science
Languages : en
Pages : 155
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Book Description
Due to the complexity and interconnectivity of many biological processes, an integrative systems biology approach is required to better understand them. The integration of various omics data (including genomics, proteomics, transcriptomics, and metabolomics data) will provide a holistic view that highlights the relationship among the various biomolecules and their functions in plant growth, resistance, quality, and many other important traits. Recent advances in analytical power and in the ability to process large data sets has enabled researchers to integrate multi-omics data and have a much deeper knowledge of the cell, tissue, organ, or even an entire organism being studied. The goal of this Research Topic is not only to publish high-quality and reliable omics data but also to answer the bigger question of how to integrate and correlate different data sets from multiple omics studies. Can one omics data set be used as a primary data set for the design and execution of another omics study? If so, then how it can be done? What are its pre-requisites? How can we plan research involving multi-omics approach to reach a meaningful conclusion? These are the questions that need our attention and their answers are critical for the future of omics-based research. For this Research Topic, we welcome contributors to submit manuscripts related to the following themes: • Studies on plant-based genomics, transcriptomics, proteomics, and metabolomics; • Development of new analytical methods for omics utilizing in plants; • Advancements in the current analytical methods for utilizing omics in plants; • Integration of multi-omics data sets; • Development of new statistical methods for data analysis.
Author: Stanislav S. Rubakhin
Publisher: Humana Press
ISBN: 9781617796968
Category : Science
Languages : en
Pages : 490
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Book Description
Mass spectrometry (MS) offers unmatched capabilities for the detection, characterization, and identification of a broad range of analytes. Mass spectrometry imaging (MSI) integrates MS data with information on the spatial distributions of the analytes, further enhancing the applicability of MS. In Mass Spectrometry Imaging: Principles and Protocols, expert practitioners from academia, industry, and the clinic contribute cutting-edge protocols describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues. Divided into three sections, this volume presents the principles of MS, current and future trends of MSI, and qualitative and quantitative protocols to measure and identify endogenous metabolites and xenobiotics. An array of MSI approaches and technologies for characterizing peptide and protein distributions are described in detail. Written in the highly successful Methods in Molecular BiologyTM series format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures. Also included are notes providing tips to avoid experimental pitfalls and helpful suggestions for method troubleshooting. Comprehensive and up-to-date, Mass Spectrometry Imaging: Principles and Protocols is written for scientists, biological and chemical engineers, and clinicians who are interested in applying MSI in their work and those who would benefit from having detailed experimental guidelines available in a single, convenient source.
