In Vitro Photodynamic Therapy Screening with Carbon Dot-protoporphyrin IX Conjugates PDF Download
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Author: Jose Ricardo Aguilar Cosme
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Jose Ricardo Aguilar Cosme
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Author: Homa Homayoni
Publisher:
ISBN:
Category : Afterglow (Physics)
Languages : en
Pages : 122
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In Photodynamic therapy (PDT) PDT, cancer destruction relies on applying a photosensitizing drug (PS) followed by light. Absorbed light can activate the PS to transfer energy to existing molecules and substrates or to oxygen to generate singlet oxygen which are highly toxic to cells. Protophorphyrin IX (PpIX) is a photosensitizers (PSs) which has FDA approval and an absorbance near the Soret band which is 10 times stronger than the absorbance in Q-band (600nm). Our goal was to design a modality to eliminate of external blue light in addition to increasing the drug's water dispersion which finally may result in enhancing PDT efficiency. The hypothesis of this study proposes an enhanced PDT efficiency through the delivery of synthesized afterglow nanoparticles (AG NPs), which may be excited by both X-ray and UV and emit blue light for a long time, even after removing the energy source; Folic acid-PpIX-conjugated NPs could also improve the water dispersion of PpIX. Afterglow alkaline earth silicates (Sr3MgSi2O8) nanoparticles doped with rear earth elements (Eu, Dy) were synthesized through this study; the best parameters to achieve a successful synthesis were investigated. the silanol groups oriented outside of the AG NPs caused a net negative surface charge (- 38.52 mV). Alkaline wet grinding decreased the NP size from 809 ± 40.9 nm to 399.5 ± 117.5 nm. The surface silanization of synthesized AG NPs was induced to introduce an NH2 functional group on the surface of AG NPs for further drug and FA conjugation. After APTES coating, the surface charge changed to -4.28 mV because NH2 oriented out of the NPs surface. Adding a new layer caused size increments to 458 ± 136.8 nm. Calculations of Conjugation efficiency (CE) proved that 100 [microgram]/ml of APTES-coated AG NPs was containing of 43.043 ± 6.42 [microgram]/ml of APTES. Protonated PpIX dicholoride with high reactive COOH groups were successfully conjugated to the surface of APTES-AG NPs which led to better water dispersion of PpIX-AG NPs and caused 20 times enhancement of the red emission intensity of PpIX- AG NPs for the concentration equal to 6.25 [microgram]/ml of free PpIX compared to the same concentration of PpIX in water; in addition, 4 times concentration decrement of drug was observed to get most intense red emission. The results of the spectrofluorophotometer confirmed that FRET had happened between APTES-AG NPs and PpIX which corresponds to the successful conjugation of PpIX and NPs. The size decreased to 232 ± 1.3 nm. Raman spectroscopy results confirmed not only PpIX but also FA were successfully conjugated to APTES-AG NPs. Ultimate NPs (FA-PpIX-APTES-AG NPs) could improve the generation of singlet oxygen 2.4% more than free PpIX for concentration of 1.5 [microgram]/ml of free PpIX. Conjugation efficiency (CE) calculation showed that in 100 [microgram]/ml PpIX-APTES-AG NPs, there was a 2.050±0.207 [microgram]/ml of conjugated PpIX and 100 [microgram]/ml PpIX-APTES-AG NPs was containing 26.87±2.998 [microgram]/ml of FA. Exposed PC3 cells to ultimate NPs (equal to 5 [microgram]/ml of free PpIX) demonstrated 30% less dark toxicity and almost 15% more toxicity after exposure to UV for 5 min compared to that of free PpIX. All mentioned results proved that the fabrication of FA-PpIX-conjugated AG NPs may introduce an acceptable solution to current challenges of PDT including weak penetration of blue light and low water dispersion of PpIX in water.
Author: Michael R. Hamblin
Publisher: Artech House
ISBN: 1596932783
Category : Medical
Languages : en
Pages : 601
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With today's focus on targeted and minimally invasive therapies, photodynamic therapy (PDT) is now being studied and used to combat many disease states and to investigate critical biological questions. This groundbreaking resource brings you the latest advances in photodynamic therapy and offers you a solid understanding of the design, delivery and dosimetry of the three basic ingredients of PDT - photosensitizers, light and oxygen. The book covers novel areas of mechanistic and innovative translational approaches. Moreover, it gives you an overview of the important medical applications of PDT, including approved treatments, clinical trials, and investigated therapies for cancer and non-malignant diseases.
Author: Mohammad Rabiee
Publisher: Morgan & Claypool Publishers
ISBN: 1643274163
Category : Science
Languages : en
Pages : 111
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Book Description
Advancement in the field of nanotechnology has revolutionized the field of medicines and pharmaceuticals in the twentieth century. The proper use of nanomaterials in medical applications requires a proper understanding of these compounds. This correct understanding, beyond the physical and chemical properties, must also have the correct logic of use. In other words, the strategic use of nanomaterials with applicable perspective can also help to advance research, but if we go forward with the current research perspective that leads to the expansion of inapplicable researches, the intrinsic importance of using these nanomaterials is eliminated. This book, considering the importance of nanomaterials and their application in medicine, as well as the significant growth of biomaterials in research fields, introduces the variables law (Rabiee's theory) for the implementation of this research and the establishment of a proper strategy. Considering that the degree of number of biomaterial and host variables follow a variety factors, and by increasing the degree of number of biomaterials and host variables, the degree of total variables also increases and as a result, performance and, consequently, biomaterial behavior in the host environment will have less control and predictive capabilities. For an external substance that is supposed to be in the human body, it must be predictable and controllable, In addition, according to the principle that the host in a fixed person does not have the ability to change, therefore, by using the simpler biomaterials (with less variables), the above goal is more accessible. It should be noted that in addition to observing biocompatibility tests for a biomaterial based on existing protocols and standards, the Applicable Compatibility (AC) parameter is also required in accordance with Rabiee's theory. This book is written in accordance with Rabiee's theory and the contents of this book should be evaluated from this perspective.
Author: Ravindra K Pandey
Publisher: World Scientific
ISBN: 9814719668
Category : Science
Languages : en
Pages : 563
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Book Description
The main objective of this book is to present the recent applications of photodynamic therapy (PDT) in treating cancer and other diseases. The limitations associated with current PDT agents, and the synthetic designs that have been used in various laboratories are also discussed. The utility of certain tumor-avid agent for cancer imaging (fluorescence, PET, MRI) is also summarized. The book also includes the use of delivery vehicles, including nanoparticles in improving the tumor-specificity of the desired agents. The book is basically focused on the translational approach of drug development. By providing certain specific examples, a clear concept of moving a 'product' from the bench to bed-side is also discussed.To have a clear concept of drug development the book is divided in three parts — Medicinal Chemistry, Mechanistic and Clinical studies. Each part includes the contributions from the leading scientists with extensive experience in the respective field. The handbook is assembled by renowned scientists Dr Dougherty, known as the father of PDT, Dr Kessel, well known for his contributions on mechanism of PDT and Dr Pandey for his inventions in developing improved agents for PDT and cancer-imaging.
Author: Md Saquib Hasnain
Publisher: Springer Nature
ISBN: 3031282639
Category : Technology & Engineering
Languages : en
Pages : 399
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Book Description
This book provides a holistic compilation on applications of carbon nanostructures, especially in advanced healthcare applications. It comprises chapters on utility of diverse multifunctional nanocarriers in drug delivery and biomedical applications, especially emphasizing on the synthesis and characterizations of nanosystems along with surface engineering approaches used for active targeting of the drugs. Moreover, the chapters also include the recent updates on the applications of the nanocarriers to fulfill the needs of various healthcare systems.
Author: Thierry Patrice
Publisher: Royal Society of Chemistry
ISBN: 1847551653
Category : Medical
Languages : en
Pages : 292
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For centuries, light has been used to cure various diseases. However, it is only recently that a new medical field has arisen. Photodynamic therapy (PDT), also known as photochemotherapy, is a fast growing technique which was initially devoted to cancer care but which is now recognised as a promising treatment technique in a variety of clinical fields. Written by recognised experts, Photodynamic Therapy provides a comprehensive explanation of what PDT is and how it has developed as a technique in areas such as the detection of lung cancer and applications in dermatology, gynaecology and neurosurgery. This book is ideal both as an introduction to PDT and as an informative text for those wishing to expand their knowledge. Practitioners in biological sciences, biotechnology and medicinal and pharmaceutical chemistry will find it an invaluable source of information.
Author: Flora Fitzgerald
Publisher: Nova Biomedical Books
ISBN: 9781536119121
Category : Photochemotherapy
Languages : en
Pages : 0
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As a new concept of cancer treatment, photodynamic therapy (PDT) has gained great attention in the last few decades. Compared to classical treatments such as surgery, chemotherapy and radiotherapy, PDT is a noninvasive, localized treatment of lesions that shows fewer side effects and has low systemic toxicity. In Chapter One, the basic mechanisms, applications and functional nanomaterials-based drug delivery systems for photodynamic therapy of cancer are reviewed. Chapter Two summarizes the application of different carbon based nanomaterials as agents for PDT and discusses current state-of-the-art use of fullerenes and their derivatives, carbon nanotubes and graphene quantum dots in PDT. Chapter Three covers the benefits and pitfalls of using chemi- and bioluminescent systems as intracellular excitation sources in PDT. Bioluminescence is a widespread natural phenomenon, which consists on emission of light resulting from the oxidation of a substrate in a reaction catalyzed by an enzyme in a biological system. Chapter Four addresses in the synthesis, characterization, and photodynamic activity of a novel hydrophobic photosensitizer 5,10,15,20-tetrakis(benzo[b]thiophene) Porphyrin (BTP) and water soluble photosensitizer 5,10,15,20-Tetrakis(7- sulfonatobenzo[b]thiophene) Porphyrin (SBTP). The authors lab is engaged in the synthesis of PDT molecules incorporating benzothiophene moiety to the meso-position of porphyrin molecules. Chapter Five discusses the Guidelines for Gastroenterological Endoscopy in Patients on Oral Antithrombotic Treatment established by the Japan Gastroenterological Endoscopy Society (JGES).These guidelines classify endoscopic interventions according to the risk of hemorrhage and specify the management of various antithrombotic drugs. Endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) are classified as high-risk procedures for hemorrhage, but PDT is not included in the guidelines. Because PDT causes ischemic necrosis of tumor tissue, the authors say this could possibly be performed safely in patients on oral antithrombotic therapy. The authors of Chapter Six developed a method of the initial concentration of protoporphyrin-IX in the operated removing glioblastoma tissue using the calibration curve of the fluorescent intensity and the known Pp-IX concentration in order to estimate of the more correlated with the cure rate.
Author: Samir Kumar
Publisher: BoD – Books on Demand
ISBN: 1839686596
Category : Technology & Engineering
Languages : en
Pages : 268
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This book describes the different methodologies for producing and synthesizing silver nanoparticles (AgNPs) of various shapes and sizes. It also provides an in-depth understanding of the new methods for characterizing and modifying the properties of AgNPs as well as their properties and applications in various fields. This book is a useful resource for a wide range of readers, including scientists, engineers, doctoral and postdoctoral fellows, and scientific professionals working in specialized fields such as medicine, nanotechnology, spectroscopy, analytical chemistry diagnostics, and plasmonics.
Author: Clare Louise Conway
Publisher:
ISBN:
Category :
Languages : en
Pages : 398
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