Immunological Techniques Using Fish Model - A Laboratory Manual PDF Download
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Author: R Dinakaran Michael
Publisher: Notion Press, Incorporated
ISBN: 9781644296332
Category :
Languages : en
Pages : 138
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Book Description
Hello there, immunology teachers of colleges and universities! Are you still struggling to design and develop laboratory exercises to demonstrate concepts of immunology to your undergrad and postgrad students using rabbits, guinea pigs and mice without a good animal house? All your problems are over. Here is an innovative and 'easy to follow' laboratory manual which has the procedures of many immunological techniques and assays using fish model. Using fish model is mainly to avoid the constraints of having and maintaining spacious air-conditioned animal house and animals like rabbits, guinea pigs and mice which are traditionally used for immunology laboratory exercises. Fishes have most of the immunological components, mechanisms and molecules found in higher vertebrates like rabbits, guinea pigs and mice though in a simpler style. Hence, fish is as an ideal vertebrate model for teaching and demonstrating the essential immunological concepts. The immunological exercises covered in this manual includes but not restricted to the basic techniques like dissection of lymphoid organs, preparation of leucocytes from these organs, antigen preparation, immunization and bleeding technique, and serum separation and assays like non-specific immune mechanisms like lysozyme activity and production of reactive oxygen by phagocytes, specific immune responses like antibody production, allograft rejection and DTH reaction, expression of immune related genes and vaccination and vaccine efficacy testing. Many of these techniques are being routinely used in institutions where the author served and also in institutions whose faculty fellows underwent training in the large number of training workshops conducted by the author.
Author: R Dinakaran Michael
Publisher: Notion Press, Incorporated
ISBN: 9781644296332
Category :
Languages : en
Pages : 138
Get Book Here
Book Description
Hello there, immunology teachers of colleges and universities! Are you still struggling to design and develop laboratory exercises to demonstrate concepts of immunology to your undergrad and postgrad students using rabbits, guinea pigs and mice without a good animal house? All your problems are over. Here is an innovative and 'easy to follow' laboratory manual which has the procedures of many immunological techniques and assays using fish model. Using fish model is mainly to avoid the constraints of having and maintaining spacious air-conditioned animal house and animals like rabbits, guinea pigs and mice which are traditionally used for immunology laboratory exercises. Fishes have most of the immunological components, mechanisms and molecules found in higher vertebrates like rabbits, guinea pigs and mice though in a simpler style. Hence, fish is as an ideal vertebrate model for teaching and demonstrating the essential immunological concepts. The immunological exercises covered in this manual includes but not restricted to the basic techniques like dissection of lymphoid organs, preparation of leucocytes from these organs, antigen preparation, immunization and bleeding technique, and serum separation and assays like non-specific immune mechanisms like lysozyme activity and production of reactive oxygen by phagocytes, specific immune responses like antibody production, allograft rejection and DTH reaction, expression of immune related genes and vaccination and vaccine efficacy testing. Many of these techniques are being routinely used in institutions where the author served and also in institutions whose faculty fellows underwent training in the large number of training workshops conducted by the author.
Author: Margaret J. Manning
Publisher: Academic Press
ISBN: 1483270327
Category : Nature
Languages : en
Pages : 388
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Book Description
Fish Immunology contains the proceedings of a symposium organized by the Fisheries Society of the British Isles, held in Plymouth, England, 11-13 July 1983. This volume contains 29 chapters and opens with a study on the prevention of disease outbreak or treatment of disease in fish farms with drugs or chemicals. Separate chapters follow on topics such as immune phenomena in Teleostei or Anura; phagocytosis in fish; the granulocytes of three elasmobranch species, namely Scyliorhinus canicula, Raja clavata and R. microcellata; and phagocytic cells in the dogfish (Scyliorhinus canicula l.); and levels of a-precipitin in two groups of wild fish: a group believed to be suffering from Ulcerative Dermal Necrosis (UDN) and a healthy group showing no external evidence of disease. Subsequent chapters deal with sequential antigenic competition in teleosts challenged with the fish-furunculosis bacterium Aeromonas salmonicida; the occurrence of vaccine uptake at the skin surface of rainbow trout; and vaccination and development of immunological memory in carp.
Author: Canadian Networking
Publisher: CRC Press
ISBN: 0429529031
Category : Medical
Languages : en
Pages : 164
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Book Description
The Manual of Immunological Methods represents the collaboration of the Canadian Network of Toxicology Centers, a non-profit network of university-based scientists dedicated to research, training, risk assessment, and communication. This manual provides detailed immunological methods that can be utilized by researchers or practitioners who want to
Author: National Research Council
Publisher: National Academies Press
ISBN: 0309154006
Category : Science
Languages : en
Pages : 246
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Book Description
A respected resource for decades, the Guide for the Care and Use of Laboratory Animals has been updated by a committee of experts, taking into consideration input from the scientific and laboratory animal communities and the public at large. The Guide incorporates new scientific information on common laboratory animals, including aquatic species, and includes extensive references. It is organized around major components of animal use: Key concepts of animal care and use. The Guide sets the framework for the humane care and use of laboratory animals. Animal care and use program. The Guide discusses the concept of a broad Program of Animal Care and Use, including roles and responsibilities of the Institutional Official, Attending Veterinarian and the Institutional Animal Care and Use Committee. Animal environment, husbandry, and management. A chapter on this topic is now divided into sections on terrestrial and aquatic animals and provides recommendations for housing and environment, husbandry, behavioral and population management, and more. Veterinary care. The Guide discusses veterinary care and the responsibilities of the Attending Veterinarian. It includes recommendations on animal procurement and transportation, preventive medicine (including animal biosecurity), and clinical care and management. The Guide addresses distress and pain recognition and relief, and issues surrounding euthanasia. Physical plant. The Guide identifies design issues, providing construction guidelines for functional areas; considerations such as drainage, vibration and noise control, and environmental monitoring; and specialized facilities for animal housing and research needs. The Guide for the Care and Use of Laboratory Animals provides a framework for the judgments required in the management of animal facilities. This updated and expanded resource of proven value will be important to scientists and researchers, veterinarians, animal care personnel, facilities managers, institutional administrators, policy makers involved in research issues, and animal welfare advocates.
Author: Neusely da Silva
Publisher: CRC Press
ISBN: 1351675842
Category : Science
Languages : en
Pages : 565
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Book Description
Microbiological Examination Methods of Food and Water (2nd edition) is an illustrated laboratory manual that provides an overview of current standard microbiological culture methods for the examination of food and water, adhered to by renowned international organizations, such as ISO, AOAC, APHA, FDA and FSIS/USDA. It includes methods for the enumeration of indicator microorganisms of general contamination, indicators of hygiene and sanitary conditions, sporeforming, spoilage fungi and pathogenic bacteria. Every chapter begins with a comprehensive, in-depth and updated bibliographic reference on the microorganism(s) dealt with in that particular section of the book. The latest facts on the taxonomic position of each group, genus or species are given, as well as clear guidelines on how to deal with changes in nomenclature on the internet. All chapters provide schematic comparisons between the methods presented, highlighting the main differences and similarities. This allows the user to choose the method that best meets his/her needs. Moreover, each chapter lists validated alternative quick methods, which, though not described in the book, may and can be used for the analysis of the microorganism(s) dealt with in that particular chapter. The didactic setup and the visualization of procedures in step-by-step schemes allow the user to quickly perceive and execute the procedure intended. Support material such as drawings, procedure schemes and laboratory sheets are available for downloading and customization. This compendium will serve as an up-to-date practical companion for laboratory professionals, technicians and research scientists, instructors, teachers and food and water analysts. Alimentary engineering, chemistry, biotechnology and biology (under)graduate students specializing in food sciences will also find the book beneficial. It is furthermore suited for use as a practical/laboratory manual for graduate courses in Food Engineering and Food Microbiology.
Author: Joanne S. Stolen
Publisher:
ISBN:
Category : Fishes
Languages : en
Pages : 246
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Book Description
Author: Andreas Radbruch
Publisher: Springer Science & Business Media
ISBN: 3662041294
Category : Science
Languages : en
Pages : 365
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Book Description
The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS
Author: T. M. Falk
Publisher: WorldFish
ISBN: 9718709878
Category : Aquaculture
Languages : en
Pages : 107
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Book Description
Author: Fred P. Meyer
Publisher:
ISBN:
Category : Fish kills
Languages : en
Pages : 138
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Book Description
Fish kills are graphic evidence of serious problems in a lake or stream. If the kill is related to the presence of toxic chemicals, there may be human health concerns, in addition to the obvious damage to the ecosystem and the fisheries resources. Depending on the cause of a fish kill, legal and economic ramifications may be involved. If the kill is caused by human or corporate actions, litigation is likely to follow, with possible court-awarded damages and assessed costs for cleanup and restoration. This manual is intended to help fisheries biologists and others to prepare for a fish kill investigation.
Author: Runjan Chetty
Publisher: Cambridge University Press
ISBN: 1316592065
Category : Medical
Languages : en
Pages : 525
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Book Description
Providing a unique A-Z guide to antibodies for immunohistology, this is an indispensable source for pathologists to ensure the correct application of immunohistochemistry in daily practice. Each entry includes commercial sources, clones, descriptions of stained proteins/epitopes, the full staining spectrum of normal and tumor tissues, staining pattern and cellular localization, the range of conditions of immunoreactivity, and pitfalls of the antibody's immunoprofile, giving pathologists a truly thorough quick-reference guide to sources, preparation and applications of specific antibodies. Appendices provide useful quick-reference tables of antibody panels for differential diagnoses, as well as summaries of diagnostic applications. Expanded from previous editions with over forty new entries, this handbook for diagnostic, therapeutic, prognostic and research applications of antibodies is an essential desktop book for practicing pathologists as well as researchers, residents and trainees.
