Immunochemical Techniques Laboratory Manual

Immunochemical Techniques Laboratory Manual PDF Author: John Goers
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 212

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Book Description
This is a laboratory manual for a course in immunochemical techniques designed to introduce students to the subject, enabling them to understand the wider applications of the techniques they are using. The text is written with easy-to-follow protocols for immunological techniques used in modern research labs and biochemical companies.

Immunochemical Techniques Laboratory Manual

Immunochemical Techniques Laboratory Manual PDF Author: John Goers
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 212

Get Book

Book Description
This is a laboratory manual for a course in immunochemical techniques designed to introduce students to the subject, enabling them to understand the wider applications of the techniques they are using. The text is written with easy-to-follow protocols for immunological techniques used in modern research labs and biochemical companies.

Immunochemical Techniques for the Identification and Estimation of Macromolecules

Immunochemical Techniques for the Identification and Estimation of Macromolecules PDF Author: Jørgen Clausen
Publisher: Elsevier Science Limited
ISBN: 9780444809094
Category : Science
Languages : en
Pages : 464

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Book Description
Rapid expansion in the field of immunochemistry has necessitated the publication of a new edition to succeed the indispensable present manual. As its predecessor, this revised edition presents you with the data and practical advice you need on the application of the rapidly expanding mass of data on immunochemistry. More specifically, the book examines the possibilities of employing immunological microtechniques in biochemical and histochemical analyses. The reader will find up-to-date information on the practical application of immunochemical analysis for laboratory use. Immunochemical Techniques especially stresses the possible errors and misinterpretations which may occur. Revised and extended chapters include: lectins, HLA-antigens, and crossed-electrophoresis. New chapters deal with: the complement system, enzyme immunoassay systems, immuno-fluorescence methods, immunoadsorbent techniques, and recent developments in methodology. Conveniently available in hardcover and paperback, this volume is unsurpassed both as a practical reference work, and as a laboratory manual.

Basic Exercises in Immunochemistry

Basic Exercises in Immunochemistry PDF Author: A. Nowotny
Publisher: Springer Science & Business Media
ISBN: 3662001489
Category : Science
Languages : en
Pages : 206

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Book Description
This book intends to be neither a complete survey of the field nor an exhaustive source of references. For these purposes, the use of the extensive compilation "Experimental Immunochemistry" by E. A. KABAT and M. M. MAYER (1962) or the excellent methodological textbook, "Methods in Immunology", by D. H. CAMPBELL, J. S. GARVEY, E. E. CREMER and D. H. SUSSDORF (1963), or the quite comprehensive series "Methods in Immunology and Immunochemistry" by C. A. WILLIAMS and M. W. CHASE (1967) are more suitable. The main purpose of this manual ist to provide students with a simple book which will introduce them to some frequently occurring problems in the three major sections of the immunochemistry of natural products. These are the isolation of the materials, the chemical analysis of the constituents and their structure, and, finally, the assays of the most important biological and immunological activities. In this manual the exercises are simplified and several shortcuts are taken in order to fit them into the framework of a teaching course. The introduction to each exercise gives a brief and elementary explanation of the reaction on which it is based. "Materials and Equipment" lists all tissues or cells, chemicals, glassware, and special equipment which must be available to carry out the exercise, although the very common laboratory tools are usually omitted from the list.

Immunological Techniques Using Fish Model - A Laboratory Manual

Immunological Techniques Using Fish Model - A Laboratory Manual PDF Author: R Dinakaran Michael
Publisher: Notion Press, Incorporated
ISBN: 9781644296332
Category :
Languages : en
Pages : 138

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Book Description
Hello there, immunology teachers of colleges and universities! Are you still struggling to design and develop laboratory exercises to demonstrate concepts of immunology to your undergrad and postgrad students using rabbits, guinea pigs and mice without a good animal house? All your problems are over. Here is an innovative and 'easy to follow' laboratory manual which has the procedures of many immunological techniques and assays using fish model. Using fish model is mainly to avoid the constraints of having and maintaining spacious air-conditioned animal house and animals like rabbits, guinea pigs and mice which are traditionally used for immunology laboratory exercises. Fishes have most of the immunological components, mechanisms and molecules found in higher vertebrates like rabbits, guinea pigs and mice though in a simpler style. Hence, fish is as an ideal vertebrate model for teaching and demonstrating the essential immunological concepts. The immunological exercises covered in this manual includes but not restricted to the basic techniques like dissection of lymphoid organs, preparation of leucocytes from these organs, antigen preparation, immunization and bleeding technique, and serum separation and assays like non-specific immune mechanisms like lysozyme activity and production of reactive oxygen by phagocytes, specific immune responses like antibody production, allograft rejection and DTH reaction, expression of immune related genes and vaccination and vaccine efficacy testing. Many of these techniques are being routinely used in institutions where the author served and also in institutions whose faculty fellows underwent training in the large number of training workshops conducted by the author.

Immunochemical Protocols

Immunochemical Protocols PDF Author: John Pound
Publisher: Humana Press
ISBN: 9780896033887
Category : Science
Languages : en
Pages : 508

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Book Description
This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins, and their various conjugates. The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities.

Immunology: Overview and Laboratory Manual

Immunology: Overview and Laboratory Manual PDF Author: Tobili Y. Sam-Yellowe
Publisher: Springer Nature
ISBN: 3030646866
Category : Medical
Languages : en
Pages : 461

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Book Description
A two-in-one text providing teaching lab students with an overview of immunology as well as a lab manual complete with current standard exercises. Section I of this book provides an overview of the immune system and immunity, and includes review questions, problem sets, case studies, inquiry-based questions, and more to provide students with a strong foundation in the field. Section II consists of twenty-two lab exercises focused on key concepts in immunology, such as antibody production, cell separation, cell function, immunoassays, Th1/Th2 cytokine detection, cell and tissue culture methods, and cell and molecular biology techniques. Appendices include safety information, suggested links and readings, and standard discipline processes, protocols, and instructions.

Immunochemical Protocols. Methods in Molecular Biology

Immunochemical Protocols. Methods in Molecular Biology PDF Author: John Pound
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Book Description
This much anticipated second edition of the best selling Immunological Protocols provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for ready use by molecular biologists. It covers the breadth of relevant established methods-from protein blotting and immunoassays through the visualization of cellular antigens and in situ hybridization-each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including conventional" and recombinant antibodies, fusion proteins, and their various conjugates. The second edition of Immunochemical Protocols brings many previously specialized techniques within the grasp of all laboratory workers. It remains, like its predecessor, "an excellent laboratory manual and reference" and opens the door to the productive use of a new generation of immunochemical reagents with exciting possibilities."

Antibodies

Antibodies PDF Author: Edward A. Greenfield
Publisher: Anchor Books
ISBN: 9781936113804
Category : SCIENCE
Languages : en
Pages : 0

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Book Description
This second edition of the now-classic lab manual Antibodies, by Harlow and Lane, has been revised, extended, and updated by Edward Greenfield of the Dana-Farber Cancer Center, with contributions from other leaders in the field. Once again, the manual is an essential resource for molecular biology, immunology, and cell culture labs on all matters relating to antibodies. The chapters on hybridomas and monoclonal antibodies have been recast with extensive new information and there are additional chapters on characterizing antibodies, antibody engineering, and flow cytometry. As in the original book, the emphasis in this second edition is on providing clear and authoritative protocols with sufficient background information and troubleshooting advice for the novice as well as the experienced investigator.

Immunohistochemistry

Immunohistochemistry PDF Author: Simon Renshaw
Publisher: Scion Publishing Ltd
ISBN: 1907904417
Category : Medical
Languages : en
Pages : 289

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Book Description
1. Introduction (S Renshaw) 2. Antibodies for immunochemistry (C Onley) 3. The selection of reporter labels (S Mardle) 4. Immunochemical staining techniques (S Renshaw) 5. Multiple immunochemical staining techniques (I Jones) 6. Confocal microscopy and immunohistochemistry (M Cuttle) 7. Ultrastructural immunochemistry (J Skepper) 8. Image capture, analysis and quantification (D Tannahill) 9. Quality assurance in immunohistochemistry (P Jackson) 10. Automated immunochemistry (E Schenck) List of suppliers; Index

Using Antibodies

Using Antibodies PDF Author: Edward Harlow
Publisher: CSHL Press
ISBN: 9780879695446
Category : Antibodies
Languages : en
Pages : 524

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Book Description