Author: Arsalan Amir Ali
Publisher:
ISBN:
Category :
Languages : en
Pages : 114
Book Description
Nostoc punctiforme is a filamentous cyanobacterium that has an immense potential to be used as a platform to produce biofuels and other high-value compounds. Their ability to synthesize lipid droplets and other valuable metabolites while eliminating the requirement for land also considerably increases their commercial prospective. N. punctiforme grows using plant-like oxygenic photosynthesis and can also differentiate into spore-like akinetes, nitrogen-fixing heterocysts, or motile hormogonia in response to stress. This unique ability to adapt to environmental stresses by differentiation also makes them a valuable model for studying cellular differentiation. A subset of the genes involved in stress responses are hypothesized to be regulated by the twelve alternative sigma factor subunits of RNA polymerase, which were previously identified in the genome (along with the housekeeping sigma, SigA). The sigma factors are known to be post translationally regulated by the anti-sigma factors, that are hypothesized to sequester the sigma factors until stress induces their release. However, unlike the sigma factors, the anti-sigma factors are well conserved at the primary sequence level. Thus, not much is known about anti-sigma factors in general or about the specific sigma/anti-sigma interactions in N. punctiforme. Seven putative anti-sigma factors were identified in N. punctiforme by sequence homology. We screened for the interaction of all thirteen sigma factors with these putative anti-sigma factors using a bacterial 2-hybrid assay (BacTH). Each of the anti-sigma factors interacted with two or more sigma factors in the BacTH analysis, but no interactions were found with group 3 sigma factors or the highly divergent sigma factor SigI. Twenty-three positive interactions were identified using the BacTH system, which were then quantified by using beta-galactosidase assays. We then tried to confirm twelve of the interactions showing the highest beta-galactosidase activity by using GST-pulldown assays and Western blotting. Two novel sigma/anti-sigma interacting pairs were identified by this study. The sigma factor SigB2 was shown to interact with the anti-sigma factor SapB2 and the sigma factor SigD was shown to interact with the anti-sigma factor SapD. These findings will help increase our understanding of complex regulatory processes that occur at the transcriptional level during cellular differentiation, and following stresses associated with production of high-value compounds in N. punctiforme.
Identification of Sigma
Author: Arsalan Amir Ali
Publisher:
ISBN:
Category :
Languages : en
Pages : 114
Book Description
Nostoc punctiforme is a filamentous cyanobacterium that has an immense potential to be used as a platform to produce biofuels and other high-value compounds. Their ability to synthesize lipid droplets and other valuable metabolites while eliminating the requirement for land also considerably increases their commercial prospective. N. punctiforme grows using plant-like oxygenic photosynthesis and can also differentiate into spore-like akinetes, nitrogen-fixing heterocysts, or motile hormogonia in response to stress. This unique ability to adapt to environmental stresses by differentiation also makes them a valuable model for studying cellular differentiation. A subset of the genes involved in stress responses are hypothesized to be regulated by the twelve alternative sigma factor subunits of RNA polymerase, which were previously identified in the genome (along with the housekeeping sigma, SigA). The sigma factors are known to be post translationally regulated by the anti-sigma factors, that are hypothesized to sequester the sigma factors until stress induces their release. However, unlike the sigma factors, the anti-sigma factors are well conserved at the primary sequence level. Thus, not much is known about anti-sigma factors in general or about the specific sigma/anti-sigma interactions in N. punctiforme. Seven putative anti-sigma factors were identified in N. punctiforme by sequence homology. We screened for the interaction of all thirteen sigma factors with these putative anti-sigma factors using a bacterial 2-hybrid assay (BacTH). Each of the anti-sigma factors interacted with two or more sigma factors in the BacTH analysis, but no interactions were found with group 3 sigma factors or the highly divergent sigma factor SigI. Twenty-three positive interactions were identified using the BacTH system, which were then quantified by using beta-galactosidase assays. We then tried to confirm twelve of the interactions showing the highest beta-galactosidase activity by using GST-pulldown assays and Western blotting. Two novel sigma/anti-sigma interacting pairs were identified by this study. The sigma factor SigB2 was shown to interact with the anti-sigma factor SapB2 and the sigma factor SigD was shown to interact with the anti-sigma factor SapD. These findings will help increase our understanding of complex regulatory processes that occur at the transcriptional level during cellular differentiation, and following stresses associated with production of high-value compounds in N. punctiforme.
Publisher:
ISBN:
Category :
Languages : en
Pages : 114
Book Description
Nostoc punctiforme is a filamentous cyanobacterium that has an immense potential to be used as a platform to produce biofuels and other high-value compounds. Their ability to synthesize lipid droplets and other valuable metabolites while eliminating the requirement for land also considerably increases their commercial prospective. N. punctiforme grows using plant-like oxygenic photosynthesis and can also differentiate into spore-like akinetes, nitrogen-fixing heterocysts, or motile hormogonia in response to stress. This unique ability to adapt to environmental stresses by differentiation also makes them a valuable model for studying cellular differentiation. A subset of the genes involved in stress responses are hypothesized to be regulated by the twelve alternative sigma factor subunits of RNA polymerase, which were previously identified in the genome (along with the housekeeping sigma, SigA). The sigma factors are known to be post translationally regulated by the anti-sigma factors, that are hypothesized to sequester the sigma factors until stress induces their release. However, unlike the sigma factors, the anti-sigma factors are well conserved at the primary sequence level. Thus, not much is known about anti-sigma factors in general or about the specific sigma/anti-sigma interactions in N. punctiforme. Seven putative anti-sigma factors were identified in N. punctiforme by sequence homology. We screened for the interaction of all thirteen sigma factors with these putative anti-sigma factors using a bacterial 2-hybrid assay (BacTH). Each of the anti-sigma factors interacted with two or more sigma factors in the BacTH analysis, but no interactions were found with group 3 sigma factors or the highly divergent sigma factor SigI. Twenty-three positive interactions were identified using the BacTH system, which were then quantified by using beta-galactosidase assays. We then tried to confirm twelve of the interactions showing the highest beta-galactosidase activity by using GST-pulldown assays and Western blotting. Two novel sigma/anti-sigma interacting pairs were identified by this study. The sigma factor SigB2 was shown to interact with the anti-sigma factor SapB2 and the sigma factor SigD was shown to interact with the anti-sigma factor SapD. These findings will help increase our understanding of complex regulatory processes that occur at the transcriptional level during cellular differentiation, and following stresses associated with production of high-value compounds in N. punctiforme.
Sigma Receptors and the Immune System
Author: Ben B. Whitlock
Publisher:
ISBN:
Category :
Languages : en
Pages : 382
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 382
Book Description
Identification of Sigma Phase in Superalloys by Correlation with Electronic and Magnetic Properties
Author: Uriah David Otting
Publisher:
ISBN:
Category : Heat resistant alloys
Languages : en
Pages : 80
Book Description
Publisher:
ISBN:
Category : Heat resistant alloys
Languages : en
Pages : 80
Book Description
Identification of the Anti-sigma Factor for Sigma Y in Bacillus Subtilis
Author: Matthew Mendel
Publisher:
ISBN:
Category : Bacillus subtilis
Languages : en
Pages : 234
Book Description
Publisher:
ISBN:
Category : Bacillus subtilis
Languages : en
Pages : 234
Book Description
Managing Emotions in Organizations
Author: Riikka Harikkala-Laihinen
Publisher: Springer Nature
ISBN: 3030605671
Category : Business & Economics
Languages : en
Pages : 135
Book Description
This book takes a fresh perspective to acquisition research, focusing on employee emotions. It builds on the human-centric approach to mergers and acquisitions, where previous literature has concluded that emotions are important, yet few studies have explored them in depth. To fill the gap, this book takes emotion research in organizations as its starting point, exploring what emotions are, how they emerge, and how they influence organizational contexts, such as acquisitions. Whereas previous acquisition literature has concluded that emotions are most often negative and lead to complications, this book shows how emotions can become a positive force driving post-acquisition change and unification. This book combines multidisciplinary theoretical insights with practical real-world case studies to provide detailed analysis and approachable findings that will appeal to academics and practitioners alike.
Publisher: Springer Nature
ISBN: 3030605671
Category : Business & Economics
Languages : en
Pages : 135
Book Description
This book takes a fresh perspective to acquisition research, focusing on employee emotions. It builds on the human-centric approach to mergers and acquisitions, where previous literature has concluded that emotions are important, yet few studies have explored them in depth. To fill the gap, this book takes emotion research in organizations as its starting point, exploring what emotions are, how they emerge, and how they influence organizational contexts, such as acquisitions. Whereas previous acquisition literature has concluded that emotions are most often negative and lead to complications, this book shows how emotions can become a positive force driving post-acquisition change and unification. This book combines multidisciplinary theoretical insights with practical real-world case studies to provide detailed analysis and approachable findings that will appeal to academics and practitioners alike.
Human Sigma
Author: Jim Asplund
Publisher: Simon and Schuster
ISBN: 1595620451
Category : Business & Economics
Languages : en
Pages : 320
Book Description
Six Sigma changed the face of manufacturing quality. Now, HumanSigma is poised to do the same for sales and service organizations. Human Sigma offers an innovative, research-based approach to one of the toughest challenges businesses face today: how to effectively manage the employee-customer encounter to drive business success. Based on research spanning 10 million employees and 10 million customers around the world, the Human Sigma approach combines a proven method for assessing the health of the employee-customer encounter with a disciplined process for improving it. Human Sigma is based on five rules to bring excellence to how employees engage and interact with customers: RULE #1: E Pluribus Unum. Employee and customer experiences must be managed together — not as separate entities. RULE #2: Feelings Are Facts. Emotions drive and shape the employee-customer encounter. RULE #3: Think Globally, Measure and Act Locally. The employee-customer encounter must be measured and managed at the local level. RULE #4: There Is One Number You Need to Know. Employee and customer engagement interact to drive enhanced financial performance. And this interaction can be quantified and summarized with a single performance metric. RULE #5: If You Pray for Potatoes, You Better Grab a Hoe. Good intentions alone do not constitute a plan of action. Sustainable improvement in the employee-customer encounter requires disciplined local action coupled with a companywide commitment to changing how employees are recruited, positioned in roles, rewarded and recognized, and importantly, how they are managed. Essential reading for global business leaders, Human Sigma shows how sales and service companies can flourish in the new global economy. It reveals a profoundly different method for managing human systems for growth. Blending strategic analysis with hands-on, practical steps and advice, Human Sigma will change how you view your work, your employees and your customers forever.
Publisher: Simon and Schuster
ISBN: 1595620451
Category : Business & Economics
Languages : en
Pages : 320
Book Description
Six Sigma changed the face of manufacturing quality. Now, HumanSigma is poised to do the same for sales and service organizations. Human Sigma offers an innovative, research-based approach to one of the toughest challenges businesses face today: how to effectively manage the employee-customer encounter to drive business success. Based on research spanning 10 million employees and 10 million customers around the world, the Human Sigma approach combines a proven method for assessing the health of the employee-customer encounter with a disciplined process for improving it. Human Sigma is based on five rules to bring excellence to how employees engage and interact with customers: RULE #1: E Pluribus Unum. Employee and customer experiences must be managed together — not as separate entities. RULE #2: Feelings Are Facts. Emotions drive and shape the employee-customer encounter. RULE #3: Think Globally, Measure and Act Locally. The employee-customer encounter must be measured and managed at the local level. RULE #4: There Is One Number You Need to Know. Employee and customer engagement interact to drive enhanced financial performance. And this interaction can be quantified and summarized with a single performance metric. RULE #5: If You Pray for Potatoes, You Better Grab a Hoe. Good intentions alone do not constitute a plan of action. Sustainable improvement in the employee-customer encounter requires disciplined local action coupled with a companywide commitment to changing how employees are recruited, positioned in roles, rewarded and recognized, and importantly, how they are managed. Essential reading for global business leaders, Human Sigma shows how sales and service companies can flourish in the new global economy. It reveals a profoundly different method for managing human systems for growth. Blending strategic analysis with hands-on, practical steps and advice, Human Sigma will change how you view your work, your employees and your customers forever.
Sigma Phase
Author: Temple University. Research Institute
Publisher:
ISBN:
Category :
Languages : en
Pages : 48
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 48
Book Description
The Sigma Phase
Author: Francis Bernard Foley
Publisher:
ISBN:
Category : Stainless steel
Languages : en
Pages : 48
Book Description
Publisher:
ISBN:
Category : Stainless steel
Languages : en
Pages : 48
Book Description
Identification of Novel Regulators of the Alternative Sigma Factor [sigma]B in Staphylococcus Aureus
Author: William A. Kwan
Publisher:
ISBN:
Category :
Languages : en
Pages : 116
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 116
Book Description
Bacterial Signal Transduction: Networks and Drug Targets
Author: Ryutaro Utsumi
Publisher: Springer Science & Business Media
ISBN: 0387788859
Category : Medical
Languages : en
Pages : 257
Book Description
This fascinating book encourages many microbiologists and students to enter the new world of signal transduction in microbiology. Over the past decade, a vast amount of exciting new information on the signal transduction pathway in bacteria has been unearthed.
Publisher: Springer Science & Business Media
ISBN: 0387788859
Category : Medical
Languages : en
Pages : 257
Book Description
This fascinating book encourages many microbiologists and students to enter the new world of signal transduction in microbiology. Over the past decade, a vast amount of exciting new information on the signal transduction pathway in bacteria has been unearthed.