Author: Dwayne William Dexter
Publisher:
ISBN:
Category : Genetic regulation
Languages : en
Pages : 186
Book Description
Identification and Characterization of a Novel Gene Affecting the Transcript Level of an ATP-binding Cassette Transporter Gene, PDR5, in Saccharomyces Cerevisiae
Author: Dwayne William Dexter
Publisher:
ISBN:
Category : Genetic regulation
Languages : en
Pages : 186
Book Description
Publisher:
ISBN:
Category : Genetic regulation
Languages : en
Pages : 186
Book Description
Handbook of Drug Screening
Author: Ramakrishna Seethala
Publisher: CRC Press
ISBN: 0824741447
Category : Medical
Languages : en
Pages : 619
Book Description
A presentation of screening techniques, modern technologies, and high-capacity instrumentation for increased productivity in the development and discovery of new drugs, chemical compounds, and targeted delivery of pharmaceuticals. It contains practical applications and examples of strategies in cell-based and cell-free screens as well as homogeneous, fluorescence, chemiluminescence, and radioactive-based technologies.
Publisher: CRC Press
ISBN: 0824741447
Category : Medical
Languages : en
Pages : 619
Book Description
A presentation of screening techniques, modern technologies, and high-capacity instrumentation for increased productivity in the development and discovery of new drugs, chemical compounds, and targeted delivery of pharmaceuticals. It contains practical applications and examples of strategies in cell-based and cell-free screens as well as homogeneous, fluorescence, chemiluminescence, and radioactive-based technologies.
Dissertation Abstracts International
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 854
Book Description
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 854
Book Description
Cumulated Index Medicus
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1860
Book Description
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1860
Book Description
American Doctoral Dissertations
Author:
Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 704
Book Description
Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 704
Book Description
Applied and Environmental Microbiology
Author:
Publisher:
ISBN:
Category : Microbial ecology
Languages : en
Pages : 1020
Book Description
Publisher:
ISBN:
Category : Microbial ecology
Languages : en
Pages : 1020
Book Description
Genetics Abstracts
Author:
Publisher:
ISBN:
Category : Genetics
Languages : en
Pages : 776
Book Description
Publisher:
ISBN:
Category : Genetics
Languages : en
Pages : 776
Book Description
Anticancer Research
Author:
Publisher:
ISBN:
Category : Cancer
Languages : en
Pages : 882
Book Description
Publisher:
ISBN:
Category : Cancer
Languages : en
Pages : 882
Book Description
Molecular Biology of the Cell
Author:
Publisher:
ISBN:
Category : Cells
Languages : en
Pages : 984
Book Description
Publisher:
ISBN:
Category : Cells
Languages : en
Pages : 984
Book Description
Genetic and Biochemical Characterization of the Signaling Interface and the Deviant ATP Binding Site in the Yeast Multidrug Transporter Pdr5
Author: Neeti Ananthaswamy
Publisher:
ISBN:
Category : ATP-binding cassette transporters
Languages : en
Pages : 92
Book Description
Pdr5 is an ABC transporter in Saccharomyces cerevisiae that facilitates xenobiotic efflux. Like all ABC transporters Pdr5 is composed of a pair of transmembrane domain (TMDs) that contain substrate binding pockets and a pair of nucleotide binding domains (NBDs) that power substrate transport. The NBD contains conserved motifs including Walker A, Walker B, Q-loop and Signature that facilitate ATP binding/hydrolysis. At each ATP binding site, an ATP molecule is sandwiched between Walker A, Walker B, Q-loop of one NBD and Signature of the other. In the Pdr family of fungal transporters, one of the ATP binding sites is canonical, known to bind/hydrolyze ATP. The other ATP binding site, however, is made up deviant residues. Hydrolysis of ATP at the NBD is thought to cause a conformational change at the TMDs. X-ray structure of Sav1866 implicates Q-loop and the intercellular loops in communication between the TMDs and the NBDs. In order to determine the signal interface in Pdr5, we evaluated the role of the Q loop residues, E244 in NBD1 and Q951 in NBD2. Surprisingly, mutation of these conserved residues exhibited only mild drug hyper sensitivity and retained significant ATPase activity. The double mutant showed a greater than additive sensitivity, indicating a functional overlap between the two Q loops. Interestingly, the reduced ATPase activity of the double mutant was equalto the single mutants. It is likely, therefore that the Q-loop residues are involved in interdomain communication. The precise function of the deviant ATP site in Pdr5 remains unclear. In order to elucidate its role, we evaluated the contribution of the deviant Walker A (C199) and a residue in the deviant signature motif (E1013). Mutating these residues to alanine exhibited only moderate drug hypersensitivity. They also retained significant ATPase activity, indicating that the deviant Walker A and Signature are not catalytic. As expected, mutating K911 in Walker A and G312 in the signature of the canonical site creates a null phenotype. This indicates that the deviant and the canonical ATP binding sites are not equivalent. We propose that the deviant site might regulate ATP hydrolysis at the canonical site.
Publisher:
ISBN:
Category : ATP-binding cassette transporters
Languages : en
Pages : 92
Book Description
Pdr5 is an ABC transporter in Saccharomyces cerevisiae that facilitates xenobiotic efflux. Like all ABC transporters Pdr5 is composed of a pair of transmembrane domain (TMDs) that contain substrate binding pockets and a pair of nucleotide binding domains (NBDs) that power substrate transport. The NBD contains conserved motifs including Walker A, Walker B, Q-loop and Signature that facilitate ATP binding/hydrolysis. At each ATP binding site, an ATP molecule is sandwiched between Walker A, Walker B, Q-loop of one NBD and Signature of the other. In the Pdr family of fungal transporters, one of the ATP binding sites is canonical, known to bind/hydrolyze ATP. The other ATP binding site, however, is made up deviant residues. Hydrolysis of ATP at the NBD is thought to cause a conformational change at the TMDs. X-ray structure of Sav1866 implicates Q-loop and the intercellular loops in communication between the TMDs and the NBDs. In order to determine the signal interface in Pdr5, we evaluated the role of the Q loop residues, E244 in NBD1 and Q951 in NBD2. Surprisingly, mutation of these conserved residues exhibited only mild drug hyper sensitivity and retained significant ATPase activity. The double mutant showed a greater than additive sensitivity, indicating a functional overlap between the two Q loops. Interestingly, the reduced ATPase activity of the double mutant was equalto the single mutants. It is likely, therefore that the Q-loop residues are involved in interdomain communication. The precise function of the deviant ATP site in Pdr5 remains unclear. In order to elucidate its role, we evaluated the contribution of the deviant Walker A (C199) and a residue in the deviant signature motif (E1013). Mutating these residues to alanine exhibited only moderate drug hypersensitivity. They also retained significant ATPase activity, indicating that the deviant Walker A and Signature are not catalytic. As expected, mutating K911 in Walker A and G312 in the signature of the canonical site creates a null phenotype. This indicates that the deviant and the canonical ATP binding sites are not equivalent. We propose that the deviant site might regulate ATP hydrolysis at the canonical site.