High Resolution Separation and Analysis of Biological Macromolecules, Part B: Applications PDF Download
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Author: Barry L. Karger
Publisher: Academic Press
ISBN:
Category : Medical
Languages : en
Pages : 604
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Book Description
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Liquid chromatography Electrophoresis Mass spectrometry
Author: Barry L. Karger
Publisher: Academic Press
ISBN:
Category : Medical
Languages : en
Pages : 604
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Book Description
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. More than 260 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences. Liquid chromatography Electrophoresis Mass spectrometry
Author: Michael L. Johnson
Publisher: Academic Press
ISBN: 0080923402
Category : Science
Languages : en
Pages : 519
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Book Description
In the past several years, there has been an explosion in the ability of biologists, molecular biologists and biochemists to collect vast amounts of data on their systems. This volume presents sophisticated methods for estimating the thermodynamic parameters of specific protein-protein, protein-DNA and small molecule interactions. The use of thermodynamics in biological research is used as an "energy book-keeping system. While the structure and function of a molecule is important, it is equally important to know what drives the energy force. These methods look to answer: What are the sources of energy that drive the function? Which of the pathways are of biological significance? As the base of macromolecular structures continues to expand through powerful techniques of molecular biology, such as X-ray crystal data and spectroscopy methods, the importance of tested and reliable methods for answering these questions will continue to expand as well.
Author:
Publisher: Academic Press
ISBN: 0080921639
Category : Science
Languages : en
Pages : 334
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Book Description
By combining the tools of organic chemistry with those of physical biochemistry and cell biology, Non-Natural Amino Acids aims to provide fundamental insights into how proteins work within the context of complex biological systems of biomedical interest. The critically acclaimed laboratory standard for 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. With more than 400 volumes published, each Methods in Enzymology volume presents material that is relevant in today's labs -- truly an essential publication for researchers in all fields of life sciences. - Demonstrates how the tools and principles of chemistry combined with the molecules and processes of living cells can be combined to create molecules with new properties and functions found neither in nature nor in the test tube - Presents new insights into the molecular mechanisms of complex biological and chemical systems that can be gained by studying the structure and function of non-natural molecules - Provides a "one-stop shop" for tried and tested essential techniques, eliminating the need to wade through untested or unreliable methods
Author:
Publisher: Academic Press
ISBN: 0080923291
Category : Science
Languages : en
Pages : 491
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Book Description
The combination of faster, more advanced computers and more quantitatively oriented biomedical researchers has recently yielded new and more precise methods for the analysis of biomedical data. These better analyses have enhanced the conclusions that can be drawn from biomedical data, and they have changed the way that experiments are designed and performed. This volume, along with previous and forthcoming 'Computer Methods' volumes for the Methods in Enzymology serial, aims to inform biomedical researchers about recent applications of modern data analysis and simulation methods as applied to biomedical research.
Author: Paul Wassarman
Publisher: Academic Press
ISBN: 0123848814
Category : Science
Languages : en
Pages : 637
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Book Description
This volume comprehensively covers new technologies and methodologies that have appeared for the study of mouse development - Update of volume 225, Guide to Techniques in Mouse Development, edited by P.M. Wassarman and M.L. DePamphilis and published in 1993 - Comprehensively covers: - new techniques for the cryopreservation of gametes and embryos - production of transgenic and null (knockout) animals (use of ES cells) - generation of conditional/inducible mutant animals, use of gene-trap mutagenesis - analysis of allele-specific expression, use of new reporter constructs - humanizing of transgenic animals - transcript profiling of mouse development - imaging of mouse development - rederivation of animals and use of mouse genomics
Author: Roya Khosravi-Far
Publisher: Elsevier
ISBN: 0080888135
Category : Science
Languages : en
Pages : 505
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Book Description
One of the major goals of researchers in the field of apoptosis is to identify targets for novel therapies in cancer, AIDS, and Alzheimer's disease. Understanding the molecular mechanisms of the various components of the apoptotic pathways is the first step to reaching this goal. The 2002 Nobel Prize in Physiology or Medicine was awarded to Sydney Brenner (United Kingdom), H. Robert Horvitz (US) and John E. Sulston (UK) "for their discoveries concerning genetic regulation of organ development and programmed cell death." Cell death is a fundamental aspect of embryonic development, normal cellular turnover and maintenance of homeostasis (maintaining a stable, constant environment) on the one hand, and aging and disease on the other. This volume addresses the significant advances with the techniques that are being used to analyze cell death. - This volume provides the necessary, trusted methods to carry out this research on these latest therapeutic techniques. Once researchers understand the molecular mechanisms of the apoptotic pathways, they can begin to develop new therapies - Presents key methods on studying tumors and how these cancer cells evade cell death - Eliminates searching through many different sources to avoid pitfalls so the same mistakes are not made over and over
Author: P. Michael Conn
Publisher: Academic Press
ISBN: 0123851157
Category : Science
Languages : en
Pages : 441
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Book Description
This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights. - This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation - The authors explain how these methods are able to provide important biological insights
Author:
Publisher: Academic Press
ISBN: 0123859298
Category : Science
Languages : en
Pages : 465
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Book Description
This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation. The authors explain how these methods are able to provide important biological insights - This volume provides descriptions of the occurrence of the UPR, methods used to assess it, pharmacological tools and other methodological approaches to analyze its impact on cellular regulation - The authors explain how these methods are able to provide important biological insights
Author:
Publisher: Academic Press
ISBN: 0123812976
Category : Science
Languages : en
Pages : 721
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Book Description
This volume of Methods in Enzymology covers the current methodology for the detection and assessment of constitutively active proteins. The chapters written by expert authors who are leaders in the field, provide hints and tricks not available in primary research publications.It is extensively referenced, with useful figures and tables throughout the volume. - Expert authors who are leaders in the field - Extensively referenced and useful figures and tables - Provides hints and tricks to facilitate reproduction of methods
Author:
Publisher: Academic Press
ISBN: 0080570070
Category : Science
Languages : en
Pages : 610
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Book Description
The critically acclaimed laboratory standard for more than 40 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect — full-text online of volume 1 onward.
