HDBK HPLC FOR SEPARATION AMINO ACIDS PEPTIDES & PROTEINS PDF Download
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Author: William S. Hancock
Publisher: CRC-Press
ISBN: 9780849335105
Category : Science
Languages : en
Pages : 514
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Book Description
Introduction to the general principles of HPLC; HPLC instrumentation; Mobile phases; Detection methods; Separation of free amino acids; Resolution of amino acids as diastereomeric derivatives.
Author: William S. Hancock
Publisher: CRC-Press
ISBN: 9780849335105
Category : Science
Languages : en
Pages : 514
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Book Description
Introduction to the general principles of HPLC; HPLC instrumentation; Mobile phases; Detection methods; Separation of free amino acids; Resolution of amino acids as diastereomeric derivatives.
Author: William S. Hancock
Publisher: CRC-Press
ISBN: 9780849335112
Category : Science
Languages : en
Pages : 544
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Book Description
All areas of high performance liquid chromatography (HPLC) relevant to the separation of amino acid and polypeptide samples are described in these comprehensive volumes. Eighty-four chapters cover the application of HPLC to research in the life sciences. Both basic information and specific details are presented on such areas as commercially available HPLC columns, ligand-exchange chromatography, peptide hormone separations, peptide antibiotic separations and drug-protein binding. Practical suggestions are given to help you avoid pitfalls which can exist in the analysis of biological materials.
Author: Taylor & Francis Group
Publisher:
ISBN: 9781315891972
Category :
Languages : en
Pages :
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Book Description
Author: Chemical Rubber Company (Cleveland, Ohio)
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Author: William S. Hancock
Publisher: CRC-Press
ISBN: 9780849335105
Category : Science
Languages : en
Pages : 489
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Book Description
Introduction to the general principles of HPLC; HPLC instrumentation; Mobile phases; Detection methods; Separation of free amino acids; Resolution of amino acids as diastereomeric derivatives.
Author: Marie-Isabel Aguilar
Publisher: Springer Science & Business Media
ISBN: 1592597424
Category : Science
Languages : en
Pages : 411
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Book Description
The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
Author: Paul T. Matsudaira
Publisher: Elsevier
ISBN: 0080924611
Category : Science
Languages : en
Pages : 205
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Book Description
Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Mass spectrophotometric characterization of proteins and peptides N-terminal sequencing of proteins with blocked N termini Internal amino acid sequence analysis after protease digestion in-gel and on-blot Improved microscale peptide purification methods Computer analysis of protein sequences New protocols tested and refined through everyday use in authors' laboratories Updated reference chapter covering all aspects of protein microsequencing
Author: Colin T. Mant
Publisher: CRC Press
ISBN: 1351440756
Category : Medical
Languages : en
Pages : 960
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Book Description
This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.
Author: Danilo Corradini
Publisher: CRC Press
ISBN: 1420016946
Category : Science
Languages : en
Pages : 715
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Book Description
High performance liquid chromatography (HPLC) is one of the most widespread analytical and preparative scale separation techniques used for both scientific investigations and industrial and biomedical analysis. Now in its second edition, this revised and updated version of the Handbook of HPLC examines the new advances made in this field since the
Author: A. Darbre
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 650
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Book Description
Protein chemistry is a wide field embracing the analysis, synthesis, sequencing and separation of peptides, proteins and individual amino acids. Practical protein chemistry is a survey of proven techniques designed primarily to be a laboratory handbook for research workers involved with proteins.
