Genetic and Biochemical Analysis of the Role of TBP Associated Factors in RNA Polymerase II Dependent Gene Transcription in the Yeast Saccharomyces Cerevisiae

Genetic and Biochemical Analysis of the Role of TBP Associated Factors in RNA Polymerase II Dependent Gene Transcription in the Yeast Saccharomyces Cerevisiae PDF Author: Steven Lee Sanders
Publisher:
ISBN:
Category : RNA polymerases
Languages : en
Pages : 200

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Dissertation Abstracts International

Dissertation Abstracts International PDF Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 902

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American Doctoral Dissertations

American Doctoral Dissertations PDF Author:
Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 776

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The Molecular Biology of the Yeast Saccharomyces, Life Cycle and Inheritance

The Molecular Biology of the Yeast Saccharomyces, Life Cycle and Inheritance PDF Author: Jeffrey N. Strathern
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 776

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RNA Polymerase III Transcription

RNA Polymerase III Transcription PDF Author: Robert J. White
Publisher: Springer Science & Business Media
ISBN: 3662035189
Category : Science
Languages : en
Pages : 274

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Book Description
This monograph reviews and summarizes the substantial body of work that has been published on the transcription by polymerase III over the past 5 years. Progress in this field has been very rapid since 1993, and this new edition incorporates all the recent developments and offers the reader a highly detailed analysis of the current state of research on this largest and most complex of the eukaryotic RNA polymerases.

Cumulated Index Medicus

Cumulated Index Medicus PDF Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 1860

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Biochemical and Genetic Analysis of Mot1, a Regulator of Basal Transcription Saccharomyces Cerevisiae

Biochemical and Genetic Analysis of Mot1, a Regulator of Basal Transcription Saccharomyces Cerevisiae PDF Author: Joanne Irene Adamkewicz
Publisher:
ISBN:
Category :
Languages : en
Pages : 338

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MECHANISTIC STUDIES OF RNA POLYMERASE II SPECIES-SPECIFIC TRANSCRIPTION INITIATION PATTERNS

MECHANISTIC STUDIES OF RNA POLYMERASE II SPECIES-SPECIFIC TRANSCRIPTION INITIATION PATTERNS PDF Author: Chen Yang
Publisher:
ISBN:
Category :
Languages : en
Pages : 213

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Book Description
The basal eukaryotic transcription machinery for protein coding genes is highly conserved from yeast to high eukaryotes. However, while human cells usually initiate at a single transcription start site approximately 30 bp downstream of a TATA element, Schizosaccharomyces pombe typically initiates at multiple sites 30-70 bp, and Saccharomyces cerevisiae 40 to 200 bp downstream of the TATA. The determinant factor(s) for the species specific initiation and the underlying mechanisms for the multiple far downstream start site utilization in yeast are not well understood. By swapping the highly purified transcription factors between S. pombe and S. cerevisiae reconstituted transcription systems, we confirmed previous observations that RNA polymerase II and/or the general transcription factor TFIIB determine the species-specific start site utilization patterns. Further genetic and biochemical assays of TFIIB chimeras indicated that RNAPII, but not TFIIB as previously proposed, determines the distinct initiation patterns not only between the two yeast systems but also between human and yeast systems. Bubble template initiation assays showed that there is an inverse correlation between the amount of negative charge in the TFIIB B-fingertip and the efficiency of the first phosphodiester bond formation. Moreover, biochemical studies indicate that multiple initiation steps, including first phosphodiester bond formation, and RNA:DNA hybrid stability determined initiation-to-elongation transition, could be modulated to regulate the far downstream start sites utilization in S. cerevisiae. A model for multiple far downstream transcription start sites formation in S. cerevisiae is proposed.

Introduction to Epigenetics

Introduction to Epigenetics PDF Author: Renato Paro
Publisher: Springer Nature
ISBN: 3030686701
Category : Science
Languages : en
Pages : 215

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Book Description
This open access textbook leads the reader from basic concepts of chromatin structure and function and RNA mechanisms to the understanding of epigenetics, imprinting, regeneration and reprogramming. The textbook treats epigenetic phenomena in animals, as well as plants. Written by four internationally known experts and senior lecturers in this field, it provides a valuable tool for Master- and PhD- students who need to comprehend the principles of epigenetics, or wish to gain a deeper knowledge in this field. After reading this book, the student will: Have an understanding of the basic toolbox of epigenetic regulation Know how genetic and epigenetic information layers are interconnected Be able to explain complex epigenetic phenomena by understanding the structures and principles of the underlying molecular mechanisms Understand how misregulated epigenetic mechanisms can lead to disease

Macromolecular Protein Complexes II: Structure and Function

Macromolecular Protein Complexes II: Structure and Function PDF Author: J. Robin Harris
Publisher: Springer Nature
ISBN: 3030281515
Category : Science
Languages : en
Pages : 657

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Book Description
This book follows on from Volume 83 in the SCBI series (“Macromolecular Protein Complexes”), and addresses several important topics (such as the Proteasome, Anaphase Promoting Complex, Ribosome and Apoptosome) that were not previously included, together with a number of additional exciting topics in this rapidly expanding field of study. Although the first SCBI Protein Complex book focused on soluble protein complexes, the second (Vol. 87)addressed Membrane Complexes, and the third (Vol. 88) put the spotlight on Viral Protein and Nucleoprotein Complexes, a number of membrane, virus and even fibrillar protein complexes have been be considered for inclusion in the present book. A further book is also under preparation that follows the same pattern, in an attempt to provide a thorough coverage of the subject. Chapter 9 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.