Author: Yishi Jin
Publisher:
ISBN:
Category :
Languages : en
Pages : 724
Book Description
Generating Dorsal-ventral Asymmetry in the Drosophila Embryo
Author: Yishi Jin
Publisher:
ISBN:
Category :
Languages : en
Pages : 724
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 724
Book Description
Generating Complex Patterns with Limited Signals
Author: Wendy Lockwood Banka
Publisher:
ISBN:
Category :
Languages : en
Pages : 356
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 356
Book Description
Dorsal-ventral Patterning and Gene Regulation in the Early Embryo of Drosophila Melanogaster
Author: Mayra Garcia
Publisher:
ISBN:
Category : Developmental genetics
Languages : en
Pages : 0
Book Description
In order for an embryo to develop and form properly, the anterior-posterior and dorsal-ventral axes must be specified. This is accomplished by controlled regulation of gene expression that allows for the activation and repression of tissue specific genes. Patterning of the Drosophila dorsal-ventral axis is an excellent model for understanding how axis specification is controlled. The dorsal-ventral axis is patterned by a nuclear gradient of Dorsal that is highest in ventral regions of the embryo. Dorsal activates genes in a concentration dependent manner to establish early patterning of the embryo. The patterns are refined by interactions between Dorsal and other activators as well as repressors in both dorsal and ventral regions of the embryo. Until recently there was only evidence for repressors acting in ventral regions of the embryo but our studies and other recent studies have provided evidence to suggest that several repressors act in dorsal regions of the embryo to refine Dorsal target genes. Here we show that an element, the A-box, previously identified in the cis-regulatory module (CRM) of the gene intermediate neuroblast defective (ind), is necessary and sufficient to mediate dorsal repression and is also involved in activation of ind. We conducted an affinity chromatography assay and identified factors that bound the A-box element. One of the factors that bound to this element, Grh, activates ind. We also identified several chromatin-remodeling factors that may function to silence ind in dorsal regions of the embryo. Our results also indicate that a second tier of repression that is independent of the A-box element, mediates repression of ind via Dpp-signaling. We extended our studies to the CRMs of ventral neuroblast defective (vnd) and short gastrulation (sog). Using a chimeric CRM repression assay, we found that strong and weak dorsal repression are also mediated by the vnd and sog CRMs, respectively. This suggests that limiting amounts of Dorsal are not sufficient to establish the dorsal borders of dorsal-ventral patterning genes as was previously believed, and rather, repressors are used to establish these borders.
Publisher:
ISBN:
Category : Developmental genetics
Languages : en
Pages : 0
Book Description
In order for an embryo to develop and form properly, the anterior-posterior and dorsal-ventral axes must be specified. This is accomplished by controlled regulation of gene expression that allows for the activation and repression of tissue specific genes. Patterning of the Drosophila dorsal-ventral axis is an excellent model for understanding how axis specification is controlled. The dorsal-ventral axis is patterned by a nuclear gradient of Dorsal that is highest in ventral regions of the embryo. Dorsal activates genes in a concentration dependent manner to establish early patterning of the embryo. The patterns are refined by interactions between Dorsal and other activators as well as repressors in both dorsal and ventral regions of the embryo. Until recently there was only evidence for repressors acting in ventral regions of the embryo but our studies and other recent studies have provided evidence to suggest that several repressors act in dorsal regions of the embryo to refine Dorsal target genes. Here we show that an element, the A-box, previously identified in the cis-regulatory module (CRM) of the gene intermediate neuroblast defective (ind), is necessary and sufficient to mediate dorsal repression and is also involved in activation of ind. We conducted an affinity chromatography assay and identified factors that bound the A-box element. One of the factors that bound to this element, Grh, activates ind. We also identified several chromatin-remodeling factors that may function to silence ind in dorsal regions of the embryo. Our results also indicate that a second tier of repression that is independent of the A-box element, mediates repression of ind via Dpp-signaling. We extended our studies to the CRMs of ventral neuroblast defective (vnd) and short gastrulation (sog). Using a chimeric CRM repression assay, we found that strong and weak dorsal repression are also mediated by the vnd and sog CRMs, respectively. This suggests that limiting amounts of Dorsal are not sufficient to establish the dorsal borders of dorsal-ventral patterning genes as was previously believed, and rather, repressors are used to establish these borders.
Polarity Inducing Signals and Signal Transduction in Dorsal-ventral Patterning in the Drosophila Embryo
Author: David Samuel Schneider
Publisher:
ISBN:
Category :
Languages : en
Pages : 304
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 304
Book Description
The Dorsal/ventral Patterning of Transcription in the Drosophila Embryo
Author: Jiandong Huang
Publisher:
ISBN:
Category :
Languages : en
Pages : 314
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 314
Book Description
Dorsal-ventral Patterning in the Drosophila Embryo
Author: Robert Paul Ray
Publisher:
ISBN:
Category : Drosophila
Languages : en
Pages : 520
Book Description
Publisher:
ISBN:
Category : Drosophila
Languages : en
Pages : 520
Book Description
Generating Pattern in the Drosophila Embryo
Author: James A. Langeland
Publisher:
ISBN:
Category :
Languages : en
Pages : 340
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 340
Book Description
Transcriptional Repression by Dorsal and Groucho During Dorsal/ventral Patterning of the Drosophila Embryo
Author: Rubén Darío Flores-Saaib
Publisher:
ISBN:
Category :
Languages : en
Pages : 320
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 320
Book Description
Regulation of Dorsal/ventral Patterning Genes by the Drosophila Morphogen Gradient Dorsal
Author: Duojia Pan
Publisher:
ISBN:
Category :
Languages : en
Pages : 214
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 214
Book Description
Investigations Into the Roles of Easter and Snake in Patterning the Dorsal-ventral Axis of the Early Drosophila Embryo
Author: Robert Kenichi Maeda
Publisher:
ISBN:
Category :
Languages : en
Pages : 266
Book Description
Publisher:
ISBN:
Category :
Languages : en
Pages : 266
Book Description