Gene Amplification in Mammalian Cells PDF Download
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Author: Rodney E. Kellems
Publisher: CRC Press
ISBN: 1000104885
Category : Science
Languages : en
Pages : 576
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Book Description
Serves as a comprehensive review to the substantial impact of gene amplification in molecular biology, genetic engineering and medical science. The book covers the mechanism of gene amplification, organization and structure of amplified genes.
Author: Rodney E. Kellems
Publisher: CRC Press
ISBN: 1000104885
Category : Science
Languages : en
Pages : 576
Get Book
Book Description
Serves as a comprehensive review to the substantial impact of gene amplification in molecular biology, genetic engineering and medical science. The book covers the mechanism of gene amplification, organization and structure of amplified genes.
Author: Rodney E. Kellems
Publisher: CRC Press
ISBN: 9780367402662
Category :
Languages : en
Pages : 568
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Book Description
Serves as a comprehensive review to the substantial impact of gene amplification in molecular biology, genetic engineering and medical science. The book covers the mechanism of gene amplification, organization and structure of amplified genes.
Author: Jeffrey H. Miller
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 188
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Book Description
Author: George M. Malacinski
Publisher: Free Press
ISBN:
Category : Science
Languages : en
Pages : 424
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Book Description
Author: Michael Kriegler
Publisher: Oxford University Press, USA
ISBN: 9780716770046
Category : Gene Expression Regulation
Languages : en
Pages : 242
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Book Description
This book presents a descriptive, comprehensive catalogue of viral and cellular cis-acting elements and the latest expression vectors for obtaining optimum expression of foreign genes introduced into mammalian cells. Included is a selection of optimized, state-of-the-art protocols for transferring genes into mammalian cells and subsequently assaying for transfer and expression of the genes of interest. This unique volume describes in detail essential techniques for propagating cells and cell lines, DNA transfer techniques, drug selection and gene amplification techniques, expression cloning, subtractive hybridization and retrovirus-mediated gene transfer techniques. Also featured are highly sensitive assays for gene transfer and expression, including detection of DNA and RNA by both isotopic and non-isotopic detection methods as well as the most sensitive non-isotopic Western techniques for the detection of proteins.
Author: Robert T. Schimke
Publisher:
ISBN:
Category : Chromosomes
Languages : en
Pages : 370
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Book Description
Author: Mohamed Al-Rubeai
Publisher: Springer Science & Business Media
ISBN: 9048122457
Category : Medical
Languages : en
Pages : 259
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Book Description
Mammalian cell lines command an effective monopoly for the production of therapeutic proteins that require post-translational modifications. This unique advantage outweighs the costs associated with mammalian cell culture, which are far grater in terms of development time and manufacturing when compared to microbial culture. The development of cell lines has undergone several advances over the years, essentially to meet the requirement to cut the time and costs associated with using such a complex hosts as production platforms. This book provides a comprehensive guide to the methodology involved in the development of cell lines and the cell engineering approach that can be employed to enhance productivity, improve cell function, glycosylation and secretion and control apoptosis. It presents an overall picture of the current topics central to expression engineering including such topics as epigenetics and the use of technologies to overcome positional dependent inactivation, the use of promoter and enhancer sequences for expression of various transgenes, site directed engineering of defined chromosomal sites, and examination of the role of eukaryotic nucleus as the controller of expression of genes that are introduced for production of a desired product. It includes a review of selection methods for high producers and an application developed by a major biopharmaceutical industry to expedite the cell line development process. The potential of cell engineering approch to enhance cell lines through the manipulation of single genes that play important roles in key metabolic and regulatory pathways is also explored throughout.
Author: Rodney E. Kellems
Publisher: CRC Press
ISBN: 1000148092
Category : Science
Languages : en
Pages : 584
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Book Description
Serves as a comprehensive review to the substantial impact of gene amplification in molecular biology, genetic engineering and medical science. The book covers the mechanism of gene amplification, organization and structure of amplified genes.
Author: Hansjörg Hauser
Publisher: Walter de Gruyter
ISBN: 3110809281
Category : Science
Languages : en
Pages : 513
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Book Description
„Hauser und Wagner haben die neuen Möglichkeiten der Mammalian Cell Biology sehr anregend dargestellt.“ Prof. Dr. Hans Fritz, Ludwig-Maximilians-Universität München
Author: Gyun Min Lee
Publisher: John Wiley & Sons
ISBN: 3527343342
Category : Science
Languages : en
Pages : 436
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Book Description
Offers a comprehensive overview of cell culture engineering, providing insight into cell engineering, systems biology approaches and processing technology In Cell Culture Engineering: Recombinant Protein Production, editors Gyun Min Lee and Helene Faustrup Kildegaard assemble top class authors to present expert coverage of topics such as: cell line development for therapeutic protein production; development of a transient gene expression upstream platform; and CHO synthetic biology. They provide readers with everything they need to know about enhancing product and bioprocess attributes using genome-scale models of CHO metabolism; omics data and mammalian systems biotechnology; perfusion culture; and much more. This all-new, up-to-date reference covers all of the important aspects of cell culture engineering, including cell engineering, system biology approaches, and processing technology. It describes the challenges in cell line development and cell engineering, e.g. via gene editing tools like CRISPR/Cas9 and with the aim to engineer glycosylation patterns. Furthermore, it gives an overview about synthetic biology approaches applied to cell culture engineering and elaborates the use of CHO cells as common cell line for protein production. In addition, the book discusses the most important aspects of production processes, including cell culture media, batch, fed-batch, and perfusion processes as well as process analytical technology, quality by design, and scale down models. -Covers key elements of cell culture engineering applied to the production of recombinant proteins for therapeutic use -Focuses on mammalian and animal cells to help highlight synthetic and systems biology approaches to cell culture engineering, exemplified by the widely used CHO cell line -Part of the renowned "Advanced Biotechnology" book series Cell Culture Engineering: Recombinant Protein Production will appeal to biotechnologists, bioengineers, life scientists, chemical engineers, and PhD students in the life sciences.
