FNR-dependent Transcriptional Regulation in Escherichia Coli PDF Download
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Author: Eva Christine Ziegelhoffer
Publisher:
ISBN:
Category :
Languages : en
Pages : 430
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Book Description
Author: Eva Christine Ziegelhoffer
Publisher:
ISBN:
Category :
Languages : en
Pages : 430
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Book Description
Author: Karl Derek Weber
Publisher:
ISBN:
Category :
Languages : en
Pages : 176
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Book Description
Author: E. C. C. Lin
Publisher: Springer Science & Business Media
ISBN: 1468486012
Category : Medical
Languages : en
Pages : 1010
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Book Description
This up-to-date guide focuses on the understanding of key regulatory mechanisms governing gene expression in Escherichia coli. Studies of E. coli not only provide the first models of gene regulation, but research continues to yield different control mechanisms.
Author: Hemali Bharat Patel
Publisher:
ISBN:
Category :
Languages : en
Pages : 392
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Book Description
Abstract: The stringent response is a response network in bacteria that is triggered by nutrient limitation and involves a global reprogramming of gene transcription, orchestrated largely by a signaling molecule guanosine tetraphosphate (ppGpp). Despite decades of studies on individual genes perturbed during the stringent response in Escherichia coli, the characteristic transcriptional profile and the role of ppGpp in affecting global transcriptional changes during the stringent response had remained to be determined systematically. We provoked the stringent response, in the strain E. coli K-12, by multiple induction pathways and identified the core transcriptional profile that is characteristic of the cellular response to ppGpp accumulation. By comparison of wild type and ppGpp-deficient E. coli mutant strains, we identified the gene expression changes that are specifically dependent upon ppGpp. We found six transcriptional regulators that are recruited in the core stringent response. We show the regulatory targets of one of these six regulators, RpoS. Over 80% of transcriptional regulation was directly or indirectly dependent upon the general stress response sigma factor, RpoS, even in presence of ppGpp. Our findings suggest that ppGpp alone is not capable of mounting a global stringent response, and it relies heavily on transcriptional regulators such as RpoS. Existing regulatory models for ppGpp-mediated regulation suggest a global redistribution of RNA polymerase (RNAP), upon binding with ppGpp, during stringent response. We have shown evidence for such redistribution of RNAP in presence of ppGpp by conducting ChIP-chip analysis of ppGpp-rich and ppGpp-deficient cells with an antibody specific to the alpha-subunit of RNAP. We also correlated transcriptional changes with a phenotype of increased viability in acidic medium, and demonstrated that it was dependent upon both ppGpp and RpoS. We have shown that ppGpp enhances long-term survival, up to two hours, in acid. Our results reveal the significance of stringent response in development of phenotypes that facilitate adaptation in acidic host environments for E. coli.
Author: Saira Khan
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 412
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Author: Markus Willard Covert
Publisher:
ISBN:
Category :
Languages : en
Pages : 434
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Book Description
Author: Weiyin Shen
Publisher:
ISBN:
Category : Escherichia coli
Languages : en
Pages : 334
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Author: Erin Leigh Mettert
Publisher:
ISBN:
Category :
Languages : en
Pages : 300
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Author: Asma Issam Hatoum
Publisher:
ISBN:
Category :
Languages : en
Pages : 302
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Author: Thomas Edward Kuhlman
Publisher:
ISBN: 9781109953855
Category :
Languages : en
Pages : 200
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Book Description
In Chapter 4, Quantitative Characteristics of Gene Regulation by Small RNA, we study quantitatively two classes of bacterial small RNAs (sRNA) in Escherichia coli. We demonstrate that sRNA provide a novel mode of gene regulation with characteristics distinct from those of protein-mediated gene regulation. These include a threshold-linear response with a tuneable threshold and a built-in capability for hierarchical cross talk.
