Fluorescence : theory, instrument, and practice

Fluorescence : theory, instrument, and practice PDF Author: Georges G. Guilbault
Publisher:
ISBN:
Category :
Languages : fr
Pages : 697

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Fluorescence : theory, instrument, and practice

Fluorescence : theory, instrument, and practice PDF Author: Georges G. Guilbault
Publisher:
ISBN:
Category :
Languages : fr
Pages : 697

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Book Description


Fluorescence: Theory, Instrumentation, and Practice

Fluorescence: Theory, Instrumentation, and Practice PDF Author: George G. Guilbault
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 744

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Fluorescence and Phosphorescence Spectroscopy

Fluorescence and Phosphorescence Spectroscopy PDF Author: Stephen G Schulman
Publisher: Elsevier
ISBN: 1483160998
Category : Science
Languages : en
Pages : 299

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Book Description
Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists.

Practical Fluorescence, Second Edition

Practical Fluorescence, Second Edition PDF Author: George G. Guilbault
Publisher: CRC Press
ISBN: 1000104443
Category : Science
Languages : en
Pages : 825

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Book Description
This is a new edition (first, 1973) of an introduction to the principles and applications of all phases of luminescence spectroscopy. Contains (all rewritten) chapters on general aspects of luminescence, instrumentation, effects of molecular structure and environment, inorganic analysis and phosphorescence. The second edition also introduces new topics such as process, applications, bioprocess monitoring and biotechnology methods, soild surface luminescence and pesticide analysis, providing expanded coverage on chemiluminescence and environmental analysis and updates information on equipment, supplies newer references and more.

Practical Fluorescence Spectroscopy

Practical Fluorescence Spectroscopy PDF Author: Zygmunt (Karol) Gryczynski
Publisher: CRC Press
ISBN: 1498752950
Category : Medical
Languages : en
Pages : 804

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Book Description
Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence. Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations Covers steady-state phenomena, time-resolved phenomena, and advanced methods Spans biophysical, biochemical, and biomedical applications Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements

Introduction to Fluorescence

Introduction to Fluorescence PDF Author: David M. Jameson
Publisher: Taylor & Francis
ISBN: 1439806047
Category : Science
Languages : en
Pages : 316

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Book Description
The phenomenon known as fluorescence is now widely used in the chemical and life sciences largely due to the development of highly sophisticated fluorescent probe chemistries and the commercial availability of these probes as well as the development of novel microscopy approaches. Introduction to Fluorescence helps readers acquire a sound understanding of basic fluorescence theory and practice. It describes general principles in a straightforward way and uses examples from a variety of disciplines to demonstrate them. In color throughout, the book takes readers through the history of important discoveries to the most current advances. It introduces the fundamentals of the fluorescence phenomenon and gives detailed examples of fluorescence applications in the molecular life sciences, including biochemistry, biophysics, clinical chemistry and diagnostics, pharmaceutical science, and cell and molecular biology. The author presents the basic theories underlying the applications and offers in-depth information on practical aspects. Along with a list of references in each chapter, the text incorporates more than 250 figures that clearly illustrate the concepts and gives the chemical structures of the most widely used fluorescent molecules. In addition, the appendix provides a "Rogue’s Gallery" of the most common errors and pitfalls to avoid.

Aquatic Organic Matter Fluorescence

Aquatic Organic Matter Fluorescence PDF Author: Paula G. Coble
Publisher: Cambridge University Press
ISBN: 0521764610
Category : Science
Languages : en
Pages : 407

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Book Description
A core text on principles, laboratory/field methodologies, and data interpretation for fluorescence applications in aquatic science, for advanced students and researchers.

Light Spectroscopy

Light Spectroscopy PDF Author: Dr David Harris
Publisher: Garland Science
ISBN: 1000101444
Category : Science
Languages : en
Pages : 193

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Book Description
Spectrophotometry and spectrofluorometry are core techniques used throughout biology and medicine. These techniques evolve continuously and this book provides information on the latest advances in spectroscopic methods.

Practical Fluorescence; Theory, Methods, and Techniques

Practical Fluorescence; Theory, Methods, and Techniques PDF Author: George G. Guilbault
Publisher:
ISBN:
Category : Science
Languages : en
Pages : 688

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Book Description


Techniques

Techniques PDF Author: Joseph R. Lakowicz
Publisher: Springer Science & Business Media
ISBN: 0306470578
Category : Science
Languages : en
Pages : 462

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Book Description
In this inaugural volume of a new series, experts in the field help biochemists, analytical chemists, spectroscopists, biophysicists, and other specialists keep up with the latest techniques and technologies available in fluorescence spectroscopy.