Fabrication of Micro- und Nanofluidic Devices for Single DNA Molcule Analysis PDF Download
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Languages : en
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Languages : en
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Author: Yuning Zhang
Publisher:
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Languages : en
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"Nanofluidic devices, containing features with dimensions of 1-100 nm, allow for the direct detection, analysis and manipulation of single molecule analytes. In particular, over the past ten years, there has been increasing interest in developing nanofluidic devices capable of analyzing DNA at the single-molecule level, with the goal of developing high throughput mapping and eventually sequencing technology. Part of this thesis will be focusing on single-molecular DNA detection using solid state nanopores. The nanopore fabrication technique via electron beam ablation will be presented. Noise reduction is affected by coating a layer of PDMS(polydimethylsiloxane) on the nanopore supporting chip. Different folding states of DNA molecules translocating through the nanopore are observed. Since the classic nanopore setup has low signal to noise ratio, we have successfully fabricated a novel micro/nanoiudic device combining nanopore detectors with nanochannels devices by embedding a nanopore inside the nanochannel. The device concept, device fabrication, theoretical analysis and preliminary results will be covered in this thesis." --
Author: 史利南
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Languages : en
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Author: Kazuma Mawatari
Publisher: World Scientific
ISBN: 1848168012
Category : Science
Languages : en
Pages : 187
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For the past decade, new research fields utilizing microfluidics have been formed. General micro-integration methods were proposed, and the supporting fundamental technologies were widely developed. These methodologies have made various applications in the fields of analytical and chemical synthesis, and their superior performances such as rapid, simple, and high efficient processing have been proved. Recently, the space is further downscaling to 101 103nm scale (we call the space extended-nano space). The extended-nano space located between the conventional nanotechnology (100 101nm) and microtechnology (>1 m), and the research tools are not well established. In addition, the extended-nano space is a transient space from single molecules to bulk condensed phase, and fluidics and chemistry are not unknown. For these purposes, basic methodologies were developed, and new specific phenomena in fluidics and chemistry were found. These new phenomena were applied to unique chemical operations such as concentration and ion selection. The new research fields which are now being created are quite different from those in microspace. Unique devices are also increasingly being reported. In this book, we describe the fundamental technologies for extended-nano space and show the unique liquid properties found in this space and applications for single molecule or cell analysis. The research area is very new and hence, exciting. In contrast to other specialized areas, the research fields require wide knowledge (chemistry, fluidics, mechanics, photonics, biology etc.) and state-of-the-art technologies (bottom-up and top-down fabrication for various hard and soft materials, precise fluidic control, single molecule detection methods, and particle surface modification methods etc.), which have not been not covered by conventional review papers or books. Therefore, researchers or students new to the field need a new book covering these fields including recent research topics, applications and problems to be solved in the future. Our motivation is to summarize the state-of-the-art technologies for research and demonstrate new chemistry and fluidics in extended-nano space for students and researchers in academia or industry. We also emphasize the potential large impact microfluidic technologies have on chemistry and biochemistry.
Author: Miao Yu
Publisher:
ISBN:
Category : DNA
Languages : en
Pages : 130
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Author: Fan-Gang Tseng
Publisher: MDPI
ISBN: 3038421464
Category : Computers
Languages : en
Pages : 167
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This book is a printed edition of the Special Issue "Micro/Nanofluidic Devices for Single Cell Analysis" that was published in Micromachines
Author: Xiujun (James) Li
Publisher: Newnes
ISBN: 0444594612
Category : Science
Languages : en
Pages : 486
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Book Description
Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip: Principles and Applications provides chemists, biophysicists, engineers, life scientists, biotechnologists, and pharmaceutical scientists with the principles behind the design, manufacture, and testing of life sciences microfluidic systems. This book serves as a reference for technologies and applications in multidisciplinary areas, with an emphasis on quickly developing or new emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology. The book offers practical guidance on how to design, analyze, fabricate, and test microfluidic devices and systems for a wide variety of applications including separations, disease detection, cellular analysis, DNA analysis, proteomics, and drug delivery. Calculations, solved problems, data tables, and design rules are provided to help researchers understand microfluidic basic theory and principles and apply this knowledge to their own unique designs. Recent advances in microfluidics and microsystems for life sciences are impacting chemistry, biophysics, molecular, cell biology, and medicine for applications that include DNA analysis, drug discovery, disease research, and biofluid and environmental monitoring. - Provides calculations, solved problems, data tables and design rules to help understand microfluidic basic theory and principles - Gives an applied understanding of the principles behind the design, manufacture, and testing of microfluidic systems - Emphasizes on quickly developing and emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology
Author: Venkata Raghavendra Subrahmanya Sarma Mokkapati
Publisher:
ISBN: 9789090261133
Category :
Languages : en
Pages :
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Author: Iddo Heller
Publisher: Springer Nature
ISBN: 1071633775
Category : Science
Languages : en
Pages : 511
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Book Description
This third edition volume expands on the previous editions with new discussions on the latest techniques and developments in the field. The chapters in this book are organized into four parts, and cover topics such as optical tweezers; single-molecule fluorescence tools; atomic force microscopy; magnetic tweezers; applications to virus protein shells, unfolding of proteins, nucleic acids, motor proteins, in vivo and in vitro; and protocols to establish specific surface interactions and perform force calibration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Single Molecule Analysis: Methods and Protocols, Third Edition is a valuable resource for all researchers who want to learn more about this exciting and still expanding field. Chapters 2, 7, 8, 9, 12, 18, and 19 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Author: Joshua Edel
Publisher: Royal Society of Chemistry
ISBN: 1849734046
Category : Science
Languages : en
Pages : 326
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Book Description
The Nanoscience and Nanotechnology Series provides a comprehensive resource of books covering key topics such as the synthesis, characterisation, performance and properties of nanostructured materials and technologies and their applications.
