Expression of Tn9-derived Chloramphenicol Acetyltransferase Gene Fusions in Bacillus Subtilis and Escherichia Coli PDF Download
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Author: Taha Ibrahim Zaghloul
Publisher:
ISBN:
Category :
Languages : en
Pages : 536
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Author: Taha Ibrahim Zaghloul
Publisher:
ISBN:
Category :
Languages : en
Pages : 536
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Author: David Scott Goldfarb
Publisher:
ISBN:
Category :
Languages : en
Pages : 314
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Book Description
Author: Colin R. Harwood
Publisher: Springer Science & Business Media
ISBN: 1489935029
Category : Medical
Languages : en
Pages : 424
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Book Description
The genus Bacillw; has a long history of importance, both from an economic point of view and as a source of experimental microorganisms. This volume critically reviews aspects of identification, molecular biology, and growth that are of impor tance for the current and anticipated future exploitation of members of this group. In addition, the volume includes a chapter on taxonomy, as the importance of good taxonomy is often not fully appreciated; on sporulation, since so many important products are produced concomitantly with this process and we are beginning to understand the mechanisms by which the process is controlled; and, finally, on the cell envelope, as we are only just beginning to appreciate the significance of dif ferences between the cell walls of gram-positive and gram-negative bacteria for productivity and processing. The commercial importance of Bacillus lies mainly in the area of enzyme pro duction for the food, drink, and detergent markets. Increasingly, however, the ability of Bacillus to secrete proteins, coupled with its regulatory acceptability, has resulted in strenuous efforts to develop species of Bacillus as hosts for the produc tion of value-added heterologous proteins. Difficulties have often been encoun tered, indicating a need to divert more resources to improving our understanding of the molecular biology of members of this grou p. Experience with Escherichia coli, a far from ideal organism from a commercial point of view, suggests that an in creased investment in Bacillus is likely ultimately to be productive.
Author: National Academy of Sciences (U.S.)
Publisher:
ISBN:
Category : Electronic journals
Languages : en
Pages : 1008
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Book Description
The Proceedings of the National Academy of Sciences (PNAS) publishes research reports, commentaries, reviews, colloquium papers, and actions of the Academy. PNAS is a multidisciplinary journal that covers the biological, physical, and social sciences.
Author: A Ganesan
Publisher: Elsevier
ISBN: 0323153097
Category : Science
Languages : en
Pages : 381
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Book Description
Molecular Cloning and Gene Regulation in Bacilli presents the proceedings of the 1981 Cetus Conference on Genetics held at Stanford University, Stanford, California. It summarizes both basic and applied aspects of bacilli genetics. It discusses significant advances made in understanding chromosome structure, gene arrangement, molecular cloning, cloned gene expression, DNA metabolism, transcription, and translation. Divided into five sessions, the book starts by discussing the DNA sequence from RNA intergenic spaces of Bacillus subtilis rRNA gene sets, the construction of a bifunctional cosmid vector of large DNA segments, and the mating system of bacilli. Molecular cloning session covers complementation system and dominance analyses in Bacillus, genetic fusion of Escherichia coli lac genes to a Bacillus subtilis promoter, and DNA cloning of B. subtilis. It also describes the construction of trimethoprim resistant B. subtilis plasmid and expression of E. coli trp genes cloned in B. subtilis. Session III encompasses chapters that discuss protein secretion by bacilli; regulation of alpha-amylase production in B. subtilis; entomocidal toxin translation of B. thuringiensis; and expression of crystal protein, heterologous, and eukaryotic genes in bacilli. Session IV focuses on various aspects of DNA metabolism of bacilli, such as the interaction of bacterial chromosome with cell membrane; plasmid DNA in competent cells and protoplasts of B. subtilis; analysis of peptides synthesized by B. subtilis mutants; and DNA repair, uptake, restriction, modification, and recombination. The final session examines species-specific translation, control of gene expression and replications in plasmids, development of expression-vector in B. subtilis, and regulatory modifications of RNA polymerase. Each chapter is presented in an experimental manner, consisting of a summary of the study, materials and methods, results, as well as references.
Author:
Publisher:
ISBN:
Category : Medicine
Languages : en
Pages : 924
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Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 566
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Author: Stanton Gelvin
Publisher: Springer Science & Business Media
ISBN: 9400909519
Category : Science
Languages : en
Pages : 444
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Book Description
During the past ten years, great advances have been made in the area of plant molecular biology. Such formerly esoteric techniques as gene transfer and plant regeneration are now routinely performed, making the dissection of regulatory elements of genes a common practice in many laboratories. Along with this new technology has come an almost bewildering array of rapidly changing techniques, often making it difficult for the novice to select and perform the technique most appropriate for answering a given biological question. In 1986, some of us felt that many of these techniques had become routine enough to warrant the publication of a laboratory manual. The manual is designed both for advanced college level laboratory courses and as a 'bench guide' for use in the scientific laboratory. Recognizing the rapidly changing nature of plant molecular biology technology, the editors have designed a laboratory manual that is both easy to use in the laboratory and which will be updated as the techniques change and new technologies are devised. Additional chapters that can replace or be added to this first edition will be published periodically. The editors recognize that many of the techniques described in this manual depend upon specialized plant genetic material, microbial strains, or recombinant plasmids. Those people desiring such material should contact the relevant authors directly. A list of the various contributors to this manual, including their addresses, is included.
Author: Chih-Kai Lin
Publisher:
ISBN:
Category :
Languages : en
Pages : 228
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Book Description
Author: C. D. Swarna Latha
Publisher: Discovery Publishing House
ISBN: 9788183561952
Category : Bioengineering
Languages : en
Pages : 368
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Book Description
The intention of the present book entitled Eco-tourism and Environmental Management is to lay stress on the operating geomorphic processes and the assemblage of resultant landforms which have fascinated the Pachmarhi hills as an Eden of Bliss and provided the nice balance of natural beauty for the development of tourism industry. Process and form are the synonym of topography and thus the topo-culture has been defined through quantitative techniques and qualitative approaches. Nature attracts the man on their beauty and if the affection is marked too deep, it may cause the arrival of their lovers many times to quench the thirst. In this respect, it is noteworthy if on earth be an eden of bliss, it is Pachmarhi a hill resort, a trekker s paradise, the queen of Satpura and the most verdant jewel of Madhya Pradesh where nature has found exquisite expression in myriad enchanting ways. The observations have been made on the basis of the topographical sheets (Survey of India) interpretation and the extensive field survey of the area. The evaluation of terrain is made through morphometric tools denoting the facts from topo sheets and the problems and prospects have been raised by the judgement of field knowledge. Effects have been made to discuss and explain the topic through scientific approaches. The book comprises seven chapters including sections subsequently. Each chapter separately stands complete in itself but it is interlinked with the succeeding one, presenting a consummate finish with its conclusion.
