Expression of the Galactose Metabolism Genes in Yeast

Expression of the Galactose Metabolism Genes in Yeast PDF Author: Bruce Alexander Citron
Publisher:
ISBN:
Category : Eukaryotic cells
Languages : en
Pages : 364

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Book Description

Expression of the Galactose Metabolism Genes in Yeast

Expression of the Galactose Metabolism Genes in Yeast PDF Author: Bruce Alexander Citron
Publisher:
ISBN:
Category : Eukaryotic cells
Languages : en
Pages : 364

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Book Description


Galactose Regulon of Yeast

Galactose Regulon of Yeast PDF Author: Paike Jayadeva Bhat
Publisher: Springer Science & Business Media
ISBN: 3540740155
Category : Science
Languages : en
Pages : 229

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Book Description
This self contained book presents a comprehensive overview of the past, present and future of the galactose regulon of yeast, the classical model system of molecular biologists. The book starts with a brief historical overview on yeast research. This is followed by molecular genetics of the galactose regulon, isolation of genes and testing of the hypotheses. Contemporary topics including genomics, evolution, binary and graded responses, and stochasticity are all addressed.

Yeast Sugar Metabolism

Yeast Sugar Metabolism PDF Author: Friedrich K. Zimmermann
Publisher: CRC Press
ISBN: 9781566764667
Category : Technology & Engineering
Languages : en
Pages : 590

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Book Description
Yeast Sugar Metabolism looks at the biomechanics, genetics, biotechnology and applications of yeast sugar. The yeast Saccharomyces cereisiae has played a central role in the evolution of microbiology biochemistry and genetics, in addition to its use of a technical microbe for the production of alcoholic beverages and leavening of dough.

The Molecular Biology of the Yeast Saccharomyces

The Molecular Biology of the Yeast Saccharomyces PDF Author: Jeffrey N. Strathern
Publisher:
ISBN:
Category : Fungi
Languages : en
Pages : 730

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Molecular Mechanisms in Yeast Carbon Metabolism

Molecular Mechanisms in Yeast Carbon Metabolism PDF Author: Jure Piškur
Publisher: Springer
ISBN: 3642550134
Category : Science
Languages : en
Pages : 328

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Book Description
Yeast is one of the most studied laboratory organisms and represents one of the most central models to understand how any eukaryote cell works. On the other hand, yeast fermentations have for millennia provided us with a variety of biotech products, like wine, beer, vitamins, and recently also with pharmaceutically active heterologous products and biofuels. A central biochemical activity in the yeast cell is the metabolism of carbon compounds, providing energy for the whole cell, and precursors for any of the final fermentation products. A complex set of genes and regulatory pathways controls the metabolism of carbon compounds, from nutrient sensing, signal transduction, transcription regulation and post-transcriptional events. Recent advances in comparative genomics and development of post-genomic tools have provided further insights into the network of genes and enzymes, and molecular mechanisms which are responsible for a balanced metabolism of carbon compounds in the yeast cell, and which could be manipulated in the laboratory to increase the yield and quality of yeast biotech products. This book provides a dozen of most comprehensive reviews on the recent developments and achievements in the field of yeast carbon metabolism, from academic studies on gene expression to biotechnology relevant topics.

Yeast Metabolic Engineering

Yeast Metabolic Engineering PDF Author: Valeria Mapelli
Publisher: Humana Press
ISBN: 9781493905621
Category : Science
Languages : en
Pages : 0

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Book Description
Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume. Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.

Genetic and Molecular Dissection of the Integration of Galactose and Glucose Signaling in Saccharomyces Cerevisiae Strains

Genetic and Molecular Dissection of the Integration of Galactose and Glucose Signaling in Saccharomyces Cerevisiae Strains PDF Author: Renan Antonio Escalante Chong
Publisher:
ISBN:
Category :
Languages : en
Pages :

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Book Description
Cells need to sense the environment in order to survive, in particular they need to detect nutrients which will provide different building blocks and energy for the cell. This task is complicated by the fact that there can be multiple sources for the same type of nutrient available for the cell. A classical example of how cells sense multiple signals is given by carbon catabolite repression in the budding yeast S. cerevisiae. In this model the preferred carbon source glucose represses the genes used to metabolize an alternative source such as galactose. This means that the preferred carbohydrate glucose is thought to inhibit the induction of galactose genes when above a threshold concentration. Instead, we show that galactose metabolic genes (GAL) induction depends on the ratio of galactose and glucose. Surprisingly, we find that a critical portion of information processing occurs upstream of the canonical components of the GAL pathway. We then explore how cells choose between different responses to the environment. Specifically, we set out to characterize the variability in the response to combinations of galactose and glucose between several natural yeast isolates. To elucidate the genetic basis of this phenotypic variation we use QTL mapping on these strains. Our study reveals that a signal transducer GAL3 plays a central role in establishing variation in GAL gene induction.Lastly, we focus on the control of transcription in the cell. Many promoters in the cell produce both a coding transcript and a divergent transcript. To identify mutants that affect transcriptional directionality we use a bidirectionalfluorescent protein reporter in the yeast nonessential gene deletion collection. We determine that chromatin assembly can regulate divergent transcription. Moreover, mutations in the chromatin assembly factor CAF-I can lead to genome wide derepression of nascent divergent transcription.

Control of Macromolecular Synthesis

Control of Macromolecular Synthesis PDF Author: Ole Maaløe
Publisher:
ISBN:
Category : Bacteria
Languages : en
Pages : 306

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Book Description


Yeast Stress Responses

Yeast Stress Responses PDF Author: Stefan Hohmann
Publisher: Springer Science & Business Media
ISBN: 3540456112
Category : Science
Languages : en
Pages : 398

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Book Description
Every cell has developed mechanisms to respond to changes in its environment and to adapt its growth and metabolism to unfavorable conditions. The unicellular eukaryote yeast has long proven as a particularly useful model system for the analysis of cellular stress responses, and the completion of the yeast genome sequence has only added to its power This volume comprehensively reviews both the basic features of the yeast genral stress response and the specific adapations to different stress types (nutrient depletion, osmotic and heat shock as well as salt and oxidative stress). It includes the latest findings in the field and discusses the implications for the analysis of stress response mechanisms in higher eukaryotes as well.

Bioreaction Engineering Principles

Bioreaction Engineering Principles PDF Author: Jens Nielsen
Publisher: Springer
ISBN: 1461507677
Category : Technology & Engineering
Languages : en
Pages : 537

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Book Description
This is the second edition of the text "Bioreaction Engineering Principles" by Jens Nielsen and John Villadsen, originally published in 1994 by Plenum Press (now part of Kluwer). Time runs fast in Biotechnology, and when Kluwer Plenum stopped reprinting the first edition and asked us to make a second, revised edition we happily accepted. A text on bioreactions written in the early 1990's will not reflect the enormous development of experimental as well as theoretical aspects of cellular reactions during the past decade. In the preface to the first edition we admitted to be newcomers in the field. One of us (JV) has had 10 more years of job training in biotechnology, and the younger author (IN) has now received international recognition for his work with the hottest topics of "modem" biotechnology. Furthermore we are happy to have induced Gunnar Liden, professor of chemical reaction engineering at our sister university in Lund, Sweden to join us as co-author of the second edition. His contribution, especially on the chemical engineering aspects of "real" bioreactors has been of the greatest value. Chapter 8 of the present edition is largely unchanged from the first edition. We wish to thank professor Martin Hjortso from LSU for his substantial help with this chapter.