Evaluation of genetic diversity of jackfruit (Artocapus heterophyllus Lam) varieties based on sequence analysis of the rbcL gene PDF Download
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Author: Prem Jose Vazhacharickal
Publisher: GRIN Verlag
ISBN: 3668383286
Category : Science
Languages : en
Pages : 75
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Book Description
Scientific Study from the year 2016 in the subject Biology - Genetics / Gene Technology, Mar Augusthinose College, language: English, abstract: Jackfruit (Artocarpus heterophyllus) is commonly grown in home gardens of tropical and sub-tropical countries. The fruit contains high levels of carbohydrates, protein, starch, calcium and vitamins. Jack fruit has diverse medicinal uses, especially anti-oxidant, anti-inflammatory, anti-microbial, anti-cancer and anti-fungal activity. Jackfruit is considered to be an underutilized fruit where most of the fruits get wasted due to ignorance, lack of post harvest technology and gaps in supply chain systems. Jackfruit contains more protein, calcium, iron, vitamins and other essential nutrients as compared to the common fruits. To find out the morphological variation among jack fruits among Kerala we conducted an elaborative survey and found out the parameters such as tree characteristics, leaf characterises or fruit characteristics of selected samples. Both qualitative and quantitative data are measured using field visits and standard methods and morphological indicators; after analysis of data there is a morphological variation among the jack fruit samples identified. Advances in the genetic markers such as RFLP and PCR based methods are more reliable for identification of genetic diversity than morphological markers although each technique has advantages and limitations. The objective of this research work was to estimate the level of genetic diversity and to assess genetic relationships among six varieties of jackfruit using the ‘rbcL gene’ based on PCR technique and RFLP markers. The partial sequence of ‘rbcL’ gene of six different Artocarpus varieties was used in the analysis. The size of amplified products was approximately 700 bp. After sequencing and sequence editing, sequence information on a 651 bp region was finally obtained for analysis. The alignment of sequences revealed two haplotypes out of 651 sites. The nucleotide frequencies are 27.96% (A), 29.47% (T/U), 19.69% (C), and 22.89% (G). Being one of the underutilized fruits in India, Artocarpus heterophyllus Lam has promising leads to further scientific research and livelihood strategies. The study of rbcL gene using PCR and RFLP seems to be a promising tool in establishing genetic diversity among jackfruit varieties.
Author: Prem Jose Vazhacharickal
Publisher: GRIN Verlag
ISBN: 3668383286
Category : Science
Languages : en
Pages : 75
Get Book Here
Book Description
Scientific Study from the year 2016 in the subject Biology - Genetics / Gene Technology, Mar Augusthinose College, language: English, abstract: Jackfruit (Artocarpus heterophyllus) is commonly grown in home gardens of tropical and sub-tropical countries. The fruit contains high levels of carbohydrates, protein, starch, calcium and vitamins. Jack fruit has diverse medicinal uses, especially anti-oxidant, anti-inflammatory, anti-microbial, anti-cancer and anti-fungal activity. Jackfruit is considered to be an underutilized fruit where most of the fruits get wasted due to ignorance, lack of post harvest technology and gaps in supply chain systems. Jackfruit contains more protein, calcium, iron, vitamins and other essential nutrients as compared to the common fruits. To find out the morphological variation among jack fruits among Kerala we conducted an elaborative survey and found out the parameters such as tree characteristics, leaf characterises or fruit characteristics of selected samples. Both qualitative and quantitative data are measured using field visits and standard methods and morphological indicators; after analysis of data there is a morphological variation among the jack fruit samples identified. Advances in the genetic markers such as RFLP and PCR based methods are more reliable for identification of genetic diversity than morphological markers although each technique has advantages and limitations. The objective of this research work was to estimate the level of genetic diversity and to assess genetic relationships among six varieties of jackfruit using the ‘rbcL gene’ based on PCR technique and RFLP markers. The partial sequence of ‘rbcL’ gene of six different Artocarpus varieties was used in the analysis. The size of amplified products was approximately 700 bp. After sequencing and sequence editing, sequence information on a 651 bp region was finally obtained for analysis. The alignment of sequences revealed two haplotypes out of 651 sites. The nucleotide frequencies are 27.96% (A), 29.47% (T/U), 19.69% (C), and 22.89% (G). Being one of the underutilized fruits in India, Artocarpus heterophyllus Lam has promising leads to further scientific research and livelihood strategies. The study of rbcL gene using PCR and RFLP seems to be a promising tool in establishing genetic diversity among jackfruit varieties.
Author: Food and Agriculture Organization of the United Nations
Publisher: Food & Agriculture Org.
ISBN: 9251084025
Category : Technology & Engineering
Languages : en
Pages : 304
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Book Description
The publication was prepared based on information provided by 86 countries, outcomes from regional and subregional consultations and commissioned thematic studies. It includes: •an overview of definitions and concepts related to Forest Genetic Resources (FGR) and a review of their value; •a description of the main drivers of changes; •the presentation of key emerging technologies; •an analysis of the current status of FGR conservation, use and related developments; •recommendations addressing the challenges and needs. By the FAO Commission on Genetic Resources for Food and Agriculture.
Author: Mehdi Razzaghi-Abyaneh
Publisher: Springer Science & Business Media
ISBN: 364238076X
Category : Science
Languages : en
Pages : 453
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Book Description
The goal of this book is to provide essential information on the use of different medicinal plants and their secondary metabolites for the treatment of various fungal diseases affecting human beings, animals and plants. It is divided in four parts: Part I examines the global distribution of plant-derived antifungal compounds, Part II deals with antifungal activities of plant metabolites, Part III includes plants used in Ayurveda and traditional systems for treating fungal diseases, and Part IV discusses the use of plant-derived products to protect plants against fungal diseases.
Author: Sueli Rodrigues
Publisher: Academic Press
ISBN: 0128031530
Category : Technology & Engineering
Languages : en
Pages : 490
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Book Description
Exotic Fruits Reference Guide is the ultimate, most complete reference work on exotic fruits from around the world. The book focuses on exotic fruit origin, botanical aspects, cultivation and harvest, physiology and biochemistry, chemical composition and nutritional value, including phenolics and antioxidant compounds. This guide is in four-color and contains images of the fruits, in addition to their regional names and geographical locations. Harvest and post-harvest conservation, as well as the potential for industrialization, are also presented as a way of stimulating interest in consumption and large scale production. - Covers exotic fruits found all over the world, described by a team of global contributors - Provides quick and easy access to botanical information, biochemistry, fruit processing and nutritional value - Features four-color images throughout for each fruit, along with its regional name and geographical location - Serves as a useful reference for researchers, industrial practitioners and students
Author: John Vaughan
Publisher: OUP Oxford
ISBN: 0191609498
Category : Science
Languages : en
Pages : 806
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Book Description
The Oxford Book of Food Plants is a beautifully illustrated compendium of facts about the plants we grow in our gardens and use in our cooking. Gorgeous botanical illustrations are accompanied by accessible yet authoritative descriptions of each plant, along with fascinating historical details and nutritive values. This is a new edition of a classic book — fully updated with the latest nutritional research, as well as beautiful new plates and descriptions of many exotic edible plants that have only recently found their way into our markets and onto our kitchen tables — it is a must-have for anyone who loves good food, cooking, and gardening.
Author: B. N. Sathyanarayana
Publisher: I. K. International Pvt Ltd
ISBN: 8189866117
Category : Botany, Experimental
Languages : en
Pages : 299
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Book Description
Plant Tissue Culture forms an integral basis of the present day biotechnology. Plant Tissue Culture: Practices and New Experimental Protocols is being brought out to fill the existing gap in the available literature on plant tissue culture, especially focusing on the aspects of practical procedures and protocols of tissue culture. This book contains important experimental techniques and gives guidance on carrying out hands-on experiences. It has been designed in a simple way, giving all the necessary procedures as a general guideline and also necessary tips to maneuver any problem encountered. These tips are based on the first hand experiences of the author while teaching and researching the techniques of plant tissue culture. A unique feature of this book is the inclusion of several techniques describing the actual protocols experimented and developed with different plant species by different scientists. A substantial number of original colored plates including fluorescence photographs standout the book. This pioneering work is valuable for the students who are looking for fresh outlook and search.
Author: A.N.M. Alamgir
Publisher: Springer
ISBN: 3319638629
Category : Medical
Languages : en
Pages : 554
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Book Description
This volume focuses on the importance of therapeutically active compounds of natural origin. Natural materials from plants, microbes, animals, marine organisms and minerals are important sources of modern drugs. Beginning with two chapters on the development and definition of the interdisciplinary field of pharmacognosy, the volume offers up-to-date information on natural and biosynthetic sources of drugs, classification of crude drugs, pharmacognosical botany, examples of medical application, WHO ́s guidelines and intellectual property rights for herbal products.
Author: Blas Manuel De Luna
Publisher: Carnegie-Mellon University Press
ISBN:
Category : Poetry
Languages : en
Pages : 72
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Book Description
A collection of poetry by Blas Manuel De Luna.
Author: Sajad Majeed Zargar
Publisher: Springer
ISBN: 9811074798
Category : Science
Languages : en
Pages : 358
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Book Description
The natural environment for plants is composed of a complex set of abiotic and biotic stresses; plant responses to these stresses are equally complex. Systems biology allows us to identify regulatory hubs in complex networks. It also examines the molecular “parts” (transcripts, proteins and metabolites) of an organism and attempts to combine them into functional networks or models that effectively describe and predict the dynamic activities of that organism in different environments. This book focuses on research advances regarding plant responses to abiotic stresses, from the physiological level to the molecular level. It highlights new insights gained from the integration of omics datasets and identifies remaining gaps in our knowledge, outlining additional focus areas for future crop improvement research. Plants have evolved a wide range of mechanisms for coping with various abiotic stresses. In many crop plants, the molecular mechanisms involved in a single type of stress tolerance have since been identified; however, in order to arrive at a holistic understanding of major and common events concerning abiotic stresses, the signaling pathways involved must also be elucidated. To date several molecules, like transcription factors and kinases, have been identified as promising candidates that are involved in crosstalk between stress signalling pathways. However, there is a need to better understand the tolerance mechanisms for different abiotic stresses by thoroughly grasping the signalling and sensing mechanisms involved. Accordingly, this book covers a range of topics, including the impacts of different abiotic stresses on plants, the molecular mechanisms leading to tolerance for different abiotic stresses, signaling cascades revealing cross-talk among various abiotic stresses, and elucidation of major candidate molecules that may provide abiotic stress tolerance in plants.
Author: Prem Jose Vazhacharickal
Publisher: GRIN Verlag
ISBN: 3668380600
Category : Science
Languages : en
Pages : 86
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Book Description
Scientific Study from the year 2017 in the subject Biology - Genetics / Gene Technology, Mar Augusthinose College, language: English, abstract: Artocarpus heterophyllus belong to the Moraceae family and are abundant in Western Ghats. The fruit provide two MJ per kg/wet weight of ripe perianth and contain high levels of carbohydrates, protein, starch, calcium and vitamins. Jackfruit has diverse medicinal uses especially for anti-oxidant, anti-inflammatory, antimicrobial, anti-cancer and anti-fungal activity. MatK (maturase K) genes are fast evolving, highly variant regions of plant chloroplast DNA that can serve as potential biomarkers for DNA coding and also in generating primers for plants with identification of unique motif regions. Advances in the genetic markers such as RFLP and PCR based methods are more reliable for identification of genetic diversity than morphological markers, although each technique has advantages and limitations. The objective of this research work was to estimate the level of genetic diversity and to assess genetic relationships among six varieties of jackfruit using ‘matK gene’ based on PCR technique and RFLP markers. The partial sequence of the ‘matK’ gene of six different Artocarpus varities was used in the analysis. The size of amplified products was approximately 700 bp. After sequencing and sequence editing, sequence information on a 674 bp region was finally obtained for analysis. The alignment of sequences revealed two haplotypes out of 674 sites. The nucleotide frequencies are 30.00% (A), 37.69% (T/U), 17.93% (C), and 14.39% (G). Being one of the underutilized fruits in India, Artocarpus heterophyllus Lam. has promising leads to further scientific researches and livelihood strategies. The study of matK gene using PCR and RFLP seems to a promising tool in establishing genetic diversity among jackfruit varities. The tree, indigenous to the Western Ghats, is an important source of nutritious food during summer season. Encouragements should be made to the marketing as well as value added food products from this underutilized fruit tree.
