Enzyme Kinetic Analysis and Mechanistic Studies by Electrospray Mass Spectrometry PDF Download
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Author: Hong Gao
Publisher:
ISBN:
Category :
Languages : en
Pages : 420
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Author: Hong Gao
Publisher:
ISBN:
Category :
Languages : en
Pages : 420
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Book Description
Author: Julian Whitelegge
Publisher: Elsevier
ISBN: 0080932037
Category : Science
Languages : en
Pages : 563
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Book Description
This book is designed to be a central text for young graduate students interested in mass spectrometry as it relates to the study of protein structure and function as well as proteomics. It is a definite must-have work for:- libraries at academic institutions with Master and Graduate programs in biochemistry, molecular biology, structural biology and proteomics- individual laboratories with interests covering these areas - libraries and individual laboratories in the pharmaceutical and biotechnology industries.*Serves as an essential reference to those working in the field*Incorporates the contributions of prominent experts *Features comprehensive coverage and a logical structure
Author: Igor A. Kaltashov
Publisher: John Wiley & Sons
ISBN: 1118232119
Category : Science
Languages : en
Pages : 312
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Book Description
The definitive guide to mass spectrometry techniques in biology and biophysics The use of mass spectrometry (MS) to study the architecture and dynamics of proteins is increasingly common within the biophysical community, and Mass Spectrometry in Structural Biology and Biophysics: Architecture, Dynamics, and Interaction of Biomolecules, Second Edition provides readers with detailed, systematic coverage of the current state of the art. Offering an unrivalled overview of modern MS-based armamentarium that can be used to solve the most challenging problems in biophysics, structural biology, and biopharmaceuticals, the book is a practical guide to understanding the role of MS techniques in biophysical research. Designed to meet the needs of both academic and industrial researchers, it makes mass spectrometry accessible to professionals in a range of fields, including biopharmaceuticals. This new edition has been significantly expanded and updated to include the most recent experimental methodologies and techniques, MS applications in biophysics and structural biology, methods for studying higher order structure and dynamics of proteins, an examination of other biopolymers and synthetic polymers, such as nucleic acids and oligosaccharides, and much more. Featuring high-quality illustrations that illuminate the concepts described in the text, as well as extensive references that enable the reader to pursue further study, Mass Spectrometry in Structural Biology and Biophysics is an indispensable resource for researchers and graduate students working in biophysics, structural biology, protein chemistry, and related fields.
Author: Pawel L. Urban
Publisher: John Wiley & Sons
ISBN: 1118887352
Category : Science
Languages : en
Pages : 376
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Book Description
Time is an important factor in physical and natural sciences. It characterizes the progress of chemical and biochemical processes. Mass spectrometry provides the means to study molecular structures by detecting gas-phase ions with the unique mass-to-charge ratios. Time-resolved mass spectrometry (TRMS) allows one to differentiate between chemical states that can be observed sequentially at different time points. Real-time mass spectrometric monitoring enables recording data continuously with a specified temporal resolution. The TRMS approaches – introduced during the past few decades – have shown temporal resolutions ranging from hours down to microseconds and beyond. This text covers the key aspects of TRMS. It introduces ion sources, mass analyzers, and interfaces utilized in time-resolved measurements; discusses the influence of data acquisition and treatment; finally, it reviews most prominent applications of TRMS – in the studies of reaction kinetics and mechanism, physicochemical phenomena, protein structure dynamics, biocatalysis, and metabolic profiling. It will assist science and engineering students to gain a basic understanding of the TRMS concept, and to recognize its usefulness. In addition, it may benefit scientists who conduct molecular studies in the areas of chemistry, physics and biology.
Author:
Publisher: Academic Press
ISBN: 0128122749
Category : Medical
Languages : en
Pages : 634
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Book Description
Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 596, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this comprehensive update include Measurement of enzyme binding isotope effects, Chemical ligation and isotope labeling to locate dynamic effects, Measurement of heavy enzyme isotope effects, Extracting kinetic isotope effects from a global analysis of reaction progress curves, KIE of metabolic flux and enzymes, Solvent and Primary KIE on Flavin Enzymes, and The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD. Readers who are interested in applying or understanding this research will find useful methods currently used for measuring isotope effects on solution and enzyme reactions. Written by pioneers of modern isotope effect research Is the only collection of modern kinetic isotope effect methods currently available
Author: Daniel L. Purich
Publisher: Elsevier
ISBN: 0080489389
Category : Science
Languages : en
Pages : 551
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Book Description
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. - Spectroscopic Detection of Reaction Intermediates - Isotopic and Kenetic Detection of Reaction Intermediates - Chemical Trapping and Inhibitor Methods for Detecting Reaction Intermediates
Author: Horacio G Pontis
Publisher: Academic Press
ISBN: 0128034033
Category : Science
Languages : en
Pages : 252
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Book Description
Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth to them. Chapters include information on how biological active protein is extracted for different cells, determination of enzymatic activity, separation of proteins by different available methods, and descriptions of analytical methods for the determination of various types of carbohydrates in photosynthetic organisms. The book contains useful protocols for researchers working on the determination of carbohydrate metabolism. The book provides foundational content as well as step-by-step guidance on how to design and conduct an experiment, including what other methodologies could be used if advanced instruments are not readily available. - Includes a variety of analytical methods and how to apply the methods using examples from specific case studies - Discusses technical information on how to characterize plant carbohydrates and sugar nucleosides - Contains easy-to-follow protocols with detailed explanations for self-guidance - Provides foundational content as well as step-by-step guidance on how to design and conduct an experiment
Author: Na Bi (Ph.D.)
Publisher:
ISBN:
Category :
Languages : en
Pages : 532
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Book Description
Author: Norberto Peporine Lopes
Publisher: Royal Society of Chemistry
ISBN: 1782625275
Category : Science
Languages : en
Pages : 312
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Book Description
Mass spectrometry is one of the most widespread technologies in chemistry and has been increasingly used in biology with the rise of omics sciences. This book summarizes some important methodological approaches in mass spectrometry and applications in the field of chemical biology. The core chapters build on basic concepts introduced in the opening chapter and explore established fields such as high throughput screening, proteomics and metabolomics. Emerging applications of mass spectrometry in elucidating biosynthetic pathways, enzyme mechanisms and protein-protein interactions are then presented. Connections between these diverse research fields are highlighted throughout. The book concludes with a discussion of databases and future perspectives. This book will be a useful tool to early chemical biology researchers wishing to incorporate mass spectrometry as a tool in their research.
Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 774
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Book Description
