Engineering of a Novel Nonribosomal Peptide Synthetase for Alanyl-glutamine Production PDF Download
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Author: Jennifer Fick Brannock
Publisher:
ISBN:
Category :
Languages : en
Pages : 184
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Book Description
Author: Jennifer Fick Brannock
Publisher:
ISBN:
Category :
Languages : en
Pages : 184
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Book Description
Author: Philipp Stephan
Publisher:
ISBN:
Category :
Languages : de
Pages : 0
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Book Description
Over the last decades, discovery rates of new antibiotics have declined while the occurrence of multi-resistant pathogens has increased, leading towards a post-antibiotic era. Many current antibiotics are natural products or derivatives, known for their excellent bioactivities but complex structures that complicate synthetic production. Many antibiotics, like penicillin and vancomycin, are peptides produced by nonribosomal peptide synthetases (NRPS). These multi-modular enzyme complexes function like assembly lines, with each module adding a specific amino acid to the peptide through the biocatalytic activities of distinct domains. Targeted NRPS engineering to alter structures of the produced peptides has faced challenges, but directed evolution of NRPS domains seems to offer a promising solution. This thesis presents a straightforward method for the directed evolution of adenylation (A) domains using LC-MS/MS detection of dipeptides in cell lysates, demonstrating its power by engineering the GrsB1 A domain from gramicidin S biosynthesis to select for L-piperazic acid (Piz) instead of its native substrate L-Pro without reducing enzyme activity. The modified antibiotic, Piz-gramicidin S, showed improved bioactivity. Despite compatibility issues between NRPS domains, kinetic analyses revealed that A domain substrate preferences ultimately determine final product ratios. Additionally, the thesis proposes a novel DNA-based approach for NRPS engineering, using zinc finger domains that bind specific DNA sequences to simplify module rearrangement by making NRPSs DNA-templated. This method offers a more efficient alternative to traditional cloning techniques by replacing the handling of large NRPS genes with much shorter sequences of the zinc finger domains. Overall, this research advances the engineering of NRPSs for developing new antibiotics with better efficacy against resistant pathogens and highlights the usefulness of directed evolution in this context.
Author: Djamel Drider
Publisher: Springer Science & Business Media
ISBN: 1441976922
Category : Science
Languages : en
Pages : 451
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Book Description
The book will provide an overview of the advancement of fundamental knowledge and applications of antimicrobial peptides in biomedical, agricultural, veterinary, food, and cosmetic products. Antimicrobial peptides stand as potentially great alternatives to current antibiotics, and most research in this newly-created area has been published in journals and other periodicals. It is the editors’ opinion that it is timely to sum up the most important achievements in the field and provide the scientific community in a reference book. The goals of this project include illustrating the achievements made so far, debating the state of the art, and drawing new perspectives.
Author: Volker F. Wendisch
Publisher: Springer Science & Business Media
ISBN: 3540485961
Category : Science
Languages : en
Pages : 418
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Book Description
This book presents the current knowledge of fundamental as well as applied microbiology of amino acids. Coverage details the amino acid biosynthetic pathways, their genetic and biochemical regulation, transport of amino acids and genomics of producing microorganisms. The book also examines the metabolic engineering of microorganisms for the biotechnological production of amino acids for use as pharmaceuticals and as food and feed additives.
Author: Christopher Walsh
Publisher: Roberts and Company Publishers
ISBN: 9780974707730
Category : Science
Languages : en
Pages : 524
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Book Description
Covering the major classes of posttranslational modifications, Posttranslational Modification of Proteins is the first comprehensive treatment of this burgeoning area of proteome diversification.
Author: Theodor Wieland
Publisher: Springer Science & Business Media
ISBN: 3642758509
Category : Science
Languages : en
Pages : 311
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Book Description
Almost two centuries ago proteins were recognized as the primary materials (proteios = primary) oflife, but the significance and wide role of peptides (from pepsis = digestion) in practically all life pro cesses has only become apparent in the last few decades. Biologi cally active peptides are now being discovered at rapid intervals in the brain and in other organs including the heart, in the skin of amphibians and many other tissues. Peptides and peptide-like compounds are found among toxins and antibiotics. It is unlikely that this process, an almost explosive broadening of the field, will come to a sudden halt. By now it is obvious that Nature has used the combination of a small to moderate number of amino acids to generate a great variety of agonists with specific and often highly sophisticated functions. Thus, peptide chemistry must be regarded as a discipline in its own right, a major branch of biochemistry, fairly separate from the chemistry of proteins. Because of the important role played by synthesis both in the study and in the practical preparation of peptides, their area can be considered as belonging to bio-organic chemistry as well. The already overwhelming and still increasing body of know ledge renders an account of the history of peptide chemistry more and more difficult. It appears therefore timely to look back, to take stock and to recall the important stages in the development of a new discipline.
Author: W. Russ Algar
Publisher: John Wiley & Sons
ISBN: 352768347X
Category : Technology & Engineering
Languages : en
Pages : 923
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Book Description
This timely, one-stop reference is the first on an emerging and interdisciplinary topic. Covering both established and recently developed ligation chemistries, the book is divided into two didactic parts: a section that focuses on the details of bioorthogonal and chemoselective ligation reactions at the level of fundamental organic chemistry, and a section that focuses on applications, particularly in the areas of chemical biology, biomaterials, and bioanalysis, highlighting the capabilities and benefits of the ligation reactions. With chapters authored by outstanding scientists who range from trailblazers in the field to young and emerging leaders, this book on a highly interdisciplinary topic will be of great interest for biochemists, biologists, materials scientists, pharmaceutical chemists, organic chemists, and many others.
Author: Norbert Sewald
Publisher: Wiley-VCH Verlag GmbH
ISBN: 9783527304059
Category : Science
Languages : en
Pages : 562
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Book Description
Peptides play a decisive role in many physiological processes, whether as neurotransmitters, hormones or antibiotics. The rapid developments in peptide research over the past few decades make it almost impossible for newcomers to gain an overview. This means an easily comprehensible yet concise introduction is vital. This unique work covers all the important aspects of this wide-ranging field in one handy volume. On the basis of the fundamental chemical and structural properties of peptides, this reference runs the gamut from analysis, the occurrence and biological importance of peptides, via chemical, biochemical and genetic methods of peptide synthesis, right up to peptide libraries, peptide design and their role in drug research. Yet this book offers much more than a mere overview of the latest level of research. An encyclopedic appendix with valuable data on more than 500 biological relevant peptides and proteins, a comprehensive register and details of further literature references make this the ideal reference for all questions regarding peptide research. For newcomers and specialists alike. On the basis of the fundamental chemical and structural properties of peptides, this reference runs the gamut from analysis, the occurrence and biological importance of peptides.
Author: Patrick O’Donoghue
Publisher: MDPI
ISBN: 3039217348
Category : Science
Languages : en
Pages : 188
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Book Description
Dear Colleagues, Synthetic biology is a broad and emerging discipline that capitalizes on recent advances in molecular biology, genetics, protein and RNA engineering and omics technologies. These technologies have transformed our ability to reveal the biology of the cell and the molecular basis of disease. This Special Issue on “Synthetic RNA and DNA Programming” features original research articles and reviews, highlighting novel aspects of basic molecular biology and the molecular mechanisms of disease that were uncovered by the application and development of novel synthetic biology-driven approaches.
Author: Hideharu Anazawa
Publisher: Springer Science & Business Media
ISBN: 4431546073
Category : Science
Languages : en
Pages : 302
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Book Description
Microbial production: From genome design to cell surface engineering affords a comprehensive review of novel technology and approaches being implemented for manufacturing microorganisms, written by specialists in both academia and industry. This book is divided into three sections: the first includes technology for improvement of fermentation strains and many supporting technologies and information; the second examines novel technology useful for analysis of cell activities, analyzing gene function, and designing genomes of producer strains; and finally, a discussion of the practical application of the techniques and success case studies in many fields of bio-production, such as microbiological production, pharmaceuticals, chemicals, foods and cosmetics.
