RNA Methodologies

RNA Methodologies PDF Author: Robert E. Farrell Jr.
Publisher: Elsevier
ISBN: 0080454763
Category : Science
Languages : en
Pages : 794

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Book Description
This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

RNA Methodologies

RNA Methodologies PDF Author: Robert E. Farrell Jr.
Publisher: Elsevier
ISBN: 0080454763
Category : Science
Languages : en
Pages : 794

Get Book Here

Book Description
This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project.RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5' and 3' RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis.* Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Electrophoresis in Stabilizing Media

Electrophoresis in Stabilizing Media PDF Author: John Whitaker
Publisher: Elsevier
ISBN: 1483220958
Category : Science
Languages : en
Pages : 433

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Book Description
Paper Chromatography and Electrophoresis, Volume I: Electrophoresis in Stabilizing Media covers the general features of electrophoresis in stabilizing media. The book includes a consideration of the factors which determine the rate of movement of the compounds in an electrical field, the factors which must be controlled in order to obtain successful results, as well as the general arrangement and types of equipment used. The text also provides a description of methods for the separation of specific classes of compounds (amines, amino acids, peptides, proteins, nucleic acids, derivatives, and related compounds, carbohydrates, and organic acids and derivatives) normally encountered by chemists. Inorganic chemists, organic chemists, clinical chemists, and biochemists will find the book invaluable.

Physical Chemical Techniques

Physical Chemical Techniques PDF Author: Dan H. Moore
Publisher: Academic Press
ISBN: 1483194892
Category : Science
Languages : en
Pages : 405

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Book Description
Physical Techniques in Biological Research, Volume II, Part A: Physical Chemical Techniques focuses on physical chemical techniques that have been most widely used in the study of molecules of biological significance. This book outlines the theoretical basis of the methods, describes the apparatus and manipulations used, and describes the applications of the techniques by examples. Organized into seven chapters, this volume begins with an overview of the basic property that makes the use of isotopes as tracers possible. This text then explains the predicted behavior during separations of chemically reacting systems by digital computer techniques. Other chapters consider the mutual diffusion in a binary system of components A and B. This book discusses as well the migration of charged particles or molecules in a liquid medium under the influence of an applied electric field. The final chapter deals with the basic units of electric potential differences. This book is a valuable resource for biological chemists.

Techniques

Techniques PDF Author: Z. Deyl
Publisher: Elsevier
ISBN: 008085818X
Category : Science
Languages : en
Pages : 411

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Book Description
Techniques

Paper Electrophoresis

Paper Electrophoresis PDF Author: G. E. W. Wolstenholme
Publisher: John Wiley & Sons
ISBN: 0470716452
Category : Science
Languages : en
Pages : 263

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Book Description
The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.

Fundamentals and Techniques

Fundamentals and Techniques PDF Author:
Publisher: Elsevier
ISBN: 0080858244
Category : Science
Languages : en
Pages : 583

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Book Description
Fundamentals and Techniques

Experiments in the Purification and Characterization of Enzymes

Experiments in the Purification and Characterization of Enzymes PDF Author: Thomas E. Crowley
Publisher: Academic Press
ISBN: 0124095933
Category : Science
Languages : en
Pages : 267

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Book Description
Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. - Offers project lab formats for students that closely simulate original research projects - Provides instructional guidance for students to design their own experiments - Includes advanced analytical techniques - Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH - Includes access to a website with additional resources for instructors

Food Protein Chemistry

Food Protein Chemistry PDF Author: Joe Regenstein
Publisher: Elsevier
ISBN: 0323153860
Category : Technology & Engineering
Languages : en
Pages : 368

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Book Description
Food Protein Chemistry: An Introduction for Food Scientists discusses food proteins and how they are studied. Proteins are both biological entities and physicochemical compounds, and they will be examined in both contexts in this volume. The chemical and physical properties of proteins will be viewed from the perspective of chemists despite the fact that their use in the food supply emphasizes their biological nature. Key topics discussed include proteins as essential to life; amino acids; protein classification; selected proteins of the most important food systems; and protein structure. The book also includes chapters on protein measurement; protein purification; and spectral techniques for the study of proteins. The book requires readers to have the equivalent of the Institute of Food Technologists requirements for undergraduate food science majors. It also assumes a knowledge of math through calculus. While primarily intended for senior and first-year graduate food science students, the text may also be useful to researchers in allied fields.

Electromigration Techniques

Electromigration Techniques PDF Author: Boguslaw Buszewski
Publisher: Springer Science & Business Media
ISBN: 3642350437
Category : Technology & Engineering
Languages : en
Pages : 368

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Book Description
The book provides the broad knowledge on electromigration techniques including: theory of CE, description of instrumentation, theory and practice in micellar electrokinetic chromatography, isotachophoresis, capillary isoelectric focusing, capillary and planar electrochromatography (including description of instrumentation and packed and monolithic column preparation), 2D-gel electrophoresis (including sample preparation) and lab-on-a-chip systems. The book also provides the most recent examples of applications including food, environmental, pharmaceutical analysis as well as proteomics.

Interfacial Forces in Aqueous Media

Interfacial Forces in Aqueous Media PDF Author: Carel J. van Oss
Publisher: CRC Press
ISBN: 1420015761
Category : Science
Languages : en
Pages : 459

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Book Description
Thoroughly revised and reorganized, the second edition of Interfacial Forces in Aqueous Media examines the role of polar interfacial and noncovalent interactions among biological and nonbiological macromolecules as well as biopolymers, particles, surfaces, cells, and both polar and apolar polymers. The book encompasses Lifshitz-van de