Test No. 222: Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei) PDF Download
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Author: OECD
Publisher: OECD Publishing
ISBN: 926407032X
Category :
Languages : en
Pages : 18
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Book Description
This Test Guideline is designed to be used for assessing the effects of chemicals in soil on the reproductive output (and other sub-lethal end points) of the earthworm species Eisenia fetida or Eisenia andrei. Adult worms are exposed to a range of ...
Author: OECD
Publisher: OECD Publishing
ISBN: 926407032X
Category :
Languages : en
Pages : 18
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Book Description
This Test Guideline is designed to be used for assessing the effects of chemicals in soil on the reproductive output (and other sub-lethal end points) of the earthworm species Eisenia fetida or Eisenia andrei. Adult worms are exposed to a range of ...
Author: Organisation for Economic Co-operation and Development
Publisher:
ISBN:
Category : Biochemical markers
Languages : en
Pages : 18
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Book Description
Author: Organisation for Economic Co-operation and Development
Publisher:
ISBN:
Category :
Languages : en
Pages : 18
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Book Description
This Test Guideline is designed to be used for assessing the effects of chemicals in soil on the reproductive output (and other sub-lethal end points) of the earthworm species Eisenia fetida or Eisenia andrei. Adult worms are exposed to a range of concentrations of the test substance either mixed into the soil or applied to the soil surface. The range of test concentrations is selected to encompass those likely to cause both sub-lethal and lethal effects over a period of eight weeks. The limit test corresponds to one dose level of 1000 mg/kg. This study includes the observation of unusual behaviour and morphology, the counting and weighing of the adult worms after the four primary weeks, the number of juveniles hatched at the end of the second 4-week period. The reproductive output of the worms exposed to the test substance is compared to that of the control(s) in order to determine the no observed effect concentration (NOEC) and/or ECx by using a regression model to estimate the concentration that would cause a x % reduction in reproductive output. The test concentrations should bracket the ECx so that the ECx then comes from interpolation rather than extrapolation.
Author: OECD
Publisher: OECD Publishing
ISBN: 9264264493
Category :
Languages : en
Pages : 21
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Book Description
This Test Guideline is designed to be used for assessing the effects of chemicals in soil on the reproductive output (and other sub-lethal end points) of the earthworm species Eisenia fetida or Eisenia andrei.
Author:
Publisher:
ISBN: 9780660442853
Category : Dendrobaena
Languages : en
Pages : 0
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Book Description
"Revised methods now recommended by Environment and Climate Change Canada for conducting biological test methods for measuring soil toxicity using earthworms (Eisenia andrei and Dendrodrilus rubidus) are described in this report. This revised version of Report EPS 1/RM/43 includes numerous updates such as: guidance for the collection, handling and testing of soils; the removal of two test species (Eisenia fetida and Lumbricus terrestris) and the addition of another test species (Dendrodrilus rubidus) specifically for testing soils from the boreal or taiga ecozones; revised test designs; and updated guidance for culturing and testing, testing with a reference toxicant, and for the statistical analysis of data. In addition, due to lack of demand and lack of sensitivity relative to the reproduction test, the 14-day acute lethality test has been removed as a test option herein. This revised report supersedes the first edition of this document published as Report EPS 1/RM/43 in June 2004 and revised in June 2007. This test method document provides detailed procedures, conditions, and guidance for preparing for and conducting each of two discrete biological test methods for measuring soil toxicity using earthworms (Eisenia andrei or Dendrodrilus rubidus). The test methods described herein are as follows: i) a 56-day test for effects on reproduction of adult earthworms exposed to one or more samples or concentrations of contaminated or potentially contaminated soil; and ii) a 48-hour test for avoidance of contaminated soil by adult earthworms"--Abstract, page iii.
Author:
Publisher:
ISBN:
Category : Soils
Languages : en
Pages : 0
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Book Description
ISO 17512-1:2008 specifies a rapid screening method for evaluating the habitat function of soils and the influence of contaminants and chemicals on earthworm behaviour.The sublethal test is a rapid method that reflects the bioavailability of contaminant mixtures in natural soils and substances spiked into soils to Eisenia fetida and Eisenia andrei. The avoidance behaviour of the worms is the measurement endpoint of the test. This test is not intended to replace the earthworm reproduction test.Two different designs (a two section unit and a six section unit) have been developed and successfully applied. Both designs are applicable to either single-concentration (e. g. for assessing the quality of a field soil) or multi-concentration (e. g. for assessing the toxicity of a spiked chemical) tests. In both cases, the earthworms are allowed to make the initial choice on which compartment, control and a treatment [in the two section test vessel between right and left side; in the six section test vessel between the (3 + 3) alternating compartments], to enter.
Author: British Standards Institution
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Author: Canada. Environment Canada
Publisher: Environment Canada
ISBN: 9780660193663
Category : Earthworms
Languages : en
Pages : 156
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Book Description
Author: British Standards Institution
Publisher:
ISBN:
Category :
Languages : en
Pages : 0
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Book Description
Author: Cornelis A. M. van Gestel
Publisher: CRC Press
ISBN: 1439830096
Category : Medical
Languages : en
Pages : 312
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Book Description
In the last decade and a half, great progress has been made in the development of concepts and models for mixture toxicity, both in human and environmental toxicology. However, due to their different protection goals, developments have often progressed in parallel but with little integration. Arguably the first book to clearly link ecotoxicology an
