Developing Chemically Mutagenized Soybean Populations for Functional Gene Analyses at the Rhg1 Locus PDF Download
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Author: Zhou Zhou
Publisher:
ISBN:
Category :
Languages : en
Pages : 118
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Book Description
Soybean (Glycine max (L.) Merr.) cyst nematode (SCN) (Heterodera glycines Ichinohe), an obligate sedentary endoparasite, is the most economically destructive pathogen in soybean production and causes over $1 billion in annual losses in the United States. Planting resistant cultivars is the primary management method to control SCN for the long-term purpose, but the nature of genetic resistance is little known. The Rhg1 (Resistance to H. glycines ) locus on chromosome 18 is found as a major quantitative trait locus (QTL) that contributes resistance to SCN. The chemical mutagen ethylmethane sulfonate (EMS) can be utilized to induce genetic mutations in soybean populations, which screened by an efficient reverse genetic strategy known as Targeting Induced Local Lesions IN Genomes (TILLING) for functional gene analyses. The objective of this study was to analyze the function of SNAP gene ( Glyma18g02590 ) at rhg1 allele from `Forrest' (`Peking'-derived SCN resistant cultivar) using TILLING. Soybean cultivar `Forrest' seeds were mutagenized with EMS and grown to generate M1 plants. M1 plants were self-pollinated to produce approximately 3000 M2 plants. Genomic DNAs were extracted from young leaves of individual M2 plants and quantified to normalize concentration of DNAs. The DNA samples were then pooled eight-fold in 96-well plates for mutations screening by TILLING. Moreover, 12 phenotypic traits including chlorophyll deficiency, leaf shape, branch architecture, seed color, seed weight, fatty acid phenotype were identified in the mutagenized population, analyzed and archived in this study.
Author: Zhou Zhou
Publisher:
ISBN:
Category :
Languages : en
Pages : 118
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Book Description
Soybean (Glycine max (L.) Merr.) cyst nematode (SCN) (Heterodera glycines Ichinohe), an obligate sedentary endoparasite, is the most economically destructive pathogen in soybean production and causes over $1 billion in annual losses in the United States. Planting resistant cultivars is the primary management method to control SCN for the long-term purpose, but the nature of genetic resistance is little known. The Rhg1 (Resistance to H. glycines ) locus on chromosome 18 is found as a major quantitative trait locus (QTL) that contributes resistance to SCN. The chemical mutagen ethylmethane sulfonate (EMS) can be utilized to induce genetic mutations in soybean populations, which screened by an efficient reverse genetic strategy known as Targeting Induced Local Lesions IN Genomes (TILLING) for functional gene analyses. The objective of this study was to analyze the function of SNAP gene ( Glyma18g02590 ) at rhg1 allele from `Forrest' (`Peking'-derived SCN resistant cultivar) using TILLING. Soybean cultivar `Forrest' seeds were mutagenized with EMS and grown to generate M1 plants. M1 plants were self-pollinated to produce approximately 3000 M2 plants. Genomic DNAs were extracted from young leaves of individual M2 plants and quantified to normalize concentration of DNAs. The DNA samples were then pooled eight-fold in 96-well plates for mutations screening by TILLING. Moreover, 12 phenotypic traits including chlorophyll deficiency, leaf shape, branch architecture, seed color, seed weight, fatty acid phenotype were identified in the mutagenized population, analyzed and archived in this study.
Author: En Huang
Publisher:
ISBN:
Category :
Languages : en
Pages : 64
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Book Description
Soybean is one of the most economically important crop species in the world. Allelic series that increase genetic variability are very important resources for crop improvement and gene function studies by reverse genetics. Moreover, mutant varieties are not required to comply with the international Genetically Modified Organism (GMO) regulations, which makes them more acceptable to consumers. Two chemical mutagenized soybean populations of approximately 2,260 and 2,450 M2 families from cultivars 'Forrest' and 'Williams 82' were successfully developed in 2007-2008. A large number of visible morphological phenotypes were identified in these mutagenized populations, such as albinism, abnormal leaflets and black seed coats, which suggested the success of generating genetic variability by mutagenesis. Additionally, a database was established consisting of the pictures of each plant at maturity, some of the morphological and yield characteristics of each plant. Targeting Induced Local Lesions IN Genomes (TILLING) was used to identify the induced mutations in this project.
Author: Henry T. Nguyen
Publisher: Springer
ISBN: 3319641980
Category : Science
Languages : en
Pages : 216
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Book Description
This book examines the application of soybean genome sequences to comparative, structural, and functional genomics. Since the availability of the soybean genome sequence has revolutionized molecular research on this important crop species, the book also describes how the genome sequence has shaped research on transposon biology and applications for gene identification, tilling and positional gene cloning. Further, the book shows how the genome sequence influences research in the areas of genetic mapping, marker development, and genome-wide association mapping for identifying important trait genes and soybean breeding. In closing, the economic and botanical aspects of the soybean are also addressed.
Author:
Publisher: Academic Press
ISBN: 012417180X
Category : Science
Languages : en
Pages : 461
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Book Description
Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 73rd volume, the series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This thematic volume features reviews on molecular and developmental aspects of the compatible plant-nematode interaction. The contributors all actively work in the field of molecular genetics and genomics of plant parasitic nematodes and nematode feeding sites. Reviews focus on molecular and physiological aspects of nematode feeding site development and includes specific chapters on nematode effectors as well as plant responses. Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences This volume features reviews of the fast moving field of compatible interaction between plants and sedentary endo-parasitic nematodes A strong focus on molecular and physiological aspects of nematode feeding site development and includes specific chapters on nematode effectors as well as plant responses
Author: Hugo Campos
Publisher: Springer Nature
ISBN: 3030286835
Category : Science
Languages : en
Pages : 524
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Book Description
This book is open access under a CC BY 4.0 license. This book provides a fresh, updated and science-based perspective on the current status and prospects of the diverse array of topics related to the potato, and was written by distinguished scientists with hands-on global experience in research aspects related to potato. The potato is the third most important global food crop in terms of consumption. Being the only vegetatively propagated species among the world’s main five staple crops creates both issues and opportunities for the potato: on the one hand, this constrains the speed of its geographic expansion and its options for international commercialization and distribution when compared with commodity crops such as maize, wheat or rice. On the other, it provides an effective insulation against speculation and unforeseen spikes in commodity prices, since the potato does not represent a good traded on global markets. These two factors highlight the underappreciated and underrated role of the potato as a dependable nutrition security crop, one that can mitigate turmoil in world food supply and demand and political instability in some developing countries. Increasingly, the global role of the potato has expanded from a profitable crop in developing countries to a crop providing income and nutrition security in developing ones. This book will appeal to academics and students of crop sciences, but also policy makers and other stakeholders involved in the potato and its contribution to humankind’s food security.
Author: Felipe Lopes da Silva
Publisher: Springer
ISBN: 3319574337
Category : Science
Languages : en
Pages : 439
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Book Description
This book was written by soybean experts to cluster in a single publication the most relevant and modern topics in soybean breeding. It is geared mainly to students and soybean breeders around the world. It is unique since it presents the challenges and opportunities faced by soybean breeders outside the temperate world.
Author: Sonia Malik
Publisher: Springer
ISBN: 3319697692
Category : Science
Languages : en
Pages : 225
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Book Description
This book provides the latest information about hairy root culture and its several applications, with special emphasis on potential of hairy roots for the production of bioactive compounds. Due to high growth rate as well as biochemical and genetic stability, it is possible to study the metabolic pathways related to production of bioactive compounds using hairy root culture. Chapters discuss the feasibility of hairy roots for plant derived natural compounds. Advantages and difficulties of hairy roots for up-scaling studies in bioreactors are included as well as successful examples of hairy root culture of plant species producing bioactive compounds used in food, flavors and pharmaceutical industry. This book is a valuable resource for researchers and students working on the area of plant natural products, phytochemistry, plant tissue culture, medicines, and drug discovery.
Author: Roberto Fritsche-Neto
Publisher: Springer Science & Business Media
ISBN: 3642330878
Category : Science
Languages : en
Pages : 166
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Book Description
Experience shows that biotic stresses occur with different levels of intensity in nearly all agricultural areas around the world. The occurrence of insects, weeds and diseases caused by fungi, bacteria or viruses may not be relevant in a specific year but they usually harm yield in most years. Global warming has shifted the paradigm of biotic stresses in most growing areas, especially in the tropical countries, sparking intense discussions in scientific forums. This book was written with the idea of collecting in a single publication the most recent advances and discoveries concerning breeding for biotic stresses, covering all major classes of biotic challenges to agriculture and food production. Accordingly, it presents the state-of-the-art in plant stresses caused by all microorganisms, weeds and insects and how to breed for them. Complementing Plant Breeding for Abiotic Stress Tolerance, this book was written for scientists and students interested in learning how to breed for biotic stress scenarios, allowing them to develop a greater understanding of the basic mechanisms of resistance to biotic stresses and develop resistant cultivars.
Author: John Jones
Publisher: Springer Science & Business Media
ISBN: 9400704348
Category : Science
Languages : en
Pages : 563
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Book Description
This book reviews developments in the molecular biology of plant-nematode interactions that have been driven by the application of genomics tools. The book will be of interest to postgraduate students and to researchers with an interest in plant nematology and/or plant pathology more generally. A series of introductory chapters provide a biological context for the detailed reviews of all areas of plant-nematode interactions that follow and ensure that the bulk of the book is accessible to the non-specialist. A final section aims to show how these fundamental studies have provided outputs of practical relevance.
Author: Gary Stacey
Publisher: Springer Science & Business Media
ISBN: 0387722998
Category : Science
Languages : en
Pages : 405
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Book Description
Soybean genomics is of great interest as one of the most economically important crops and a major food source. This book covers recent advances in soybean genome research, including classical, RFLP, SSR, and SNP markers; genomic and cDNA libraries; functional genomics platforms; genetic and physical maps; and gene expression profiles. The book is for researchers and students in plant genetics and genomics, plant biology and pathology, agronomy, and food sciences.
