Detection and Typing Strategies for Pathogenic Escherichia coli PDF Download
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Author: Lucia Rivas
Publisher: Springer
ISBN: 1493923463
Category : Technology & Engineering
Languages : en
Pages : 114
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Book Description
This Brief will review the methods that are currently available for the detection, isolation, and typing of pathogenic E. coli with a particular focus on foodborne diseases caused by the Shiga toxigenic E. coli group, which have been implicated in a number of significant outbreaks in recent years. Pathogenic forms of E. coli can cause a variety of diarrheal diseases in hosts due to the presence of specific colonization and virulence factors, and pathogenicity-associated genes, which are generally not present in other E. coli. Six pathotypes of pathogenic E. coli are recognized (Shiga toxigenic E. coli, Enteropathogenic E. coli, Enterotoxigenic E. coli, Enteroinvasive E. coli, Enteroaggregative E. coli and Diffusely Adherent E. coli) and certain strains among these groups are major public health concerns due to the severity of disease that they can cause. Methods to detect and isolate these pathogens from a variety of sources are constantly evolving. In addition, the accumulation of knowledge on these pathogens allows for improved intervention strategies.
Author: Lucia Rivas
Publisher: Springer
ISBN: 1493923463
Category : Technology & Engineering
Languages : en
Pages : 114
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Book Description
This Brief will review the methods that are currently available for the detection, isolation, and typing of pathogenic E. coli with a particular focus on foodborne diseases caused by the Shiga toxigenic E. coli group, which have been implicated in a number of significant outbreaks in recent years. Pathogenic forms of E. coli can cause a variety of diarrheal diseases in hosts due to the presence of specific colonization and virulence factors, and pathogenicity-associated genes, which are generally not present in other E. coli. Six pathotypes of pathogenic E. coli are recognized (Shiga toxigenic E. coli, Enteropathogenic E. coli, Enterotoxigenic E. coli, Enteroinvasive E. coli, Enteroaggregative E. coli and Diffusely Adherent E. coli) and certain strains among these groups are major public health concerns due to the severity of disease that they can cause. Methods to detect and isolate these pathogens from a variety of sources are constantly evolving. In addition, the accumulation of knowledge on these pathogens allows for improved intervention strategies.
Author: Pina M. Fratamico
Publisher: Frontiers Media SA
ISBN: 2889454339
Category :
Languages : en
Pages : 172
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Book Description
Pathogenic Escherichia coli strains cause a large number of diseases in humans, including diarrhea, hemorrhagic colitis, hemolytic uremic syndrome, urinary tract infections, and neonatal meningitis, while in animals they cause diseases such as calf scours and mastitis in cattle, post-weaning diarrhea and edema disease in pigs, and peritonitis and airsacculitis in chickens. The different E. coli pathotypes are characterized by the presence of specific sets of virulence-related genes. Therefore, it is not surprising that pathogenic E. coli constitutes a genetically heterogeneous family of bacteria, and they are continuing to evolve. Rapid and accurate molecular methods are critically needed to detect and trace pathogenic E. coli in food and animals. They are also needed for epidemiological investigations to enhance food safety, as well as animal and human health and to minimize the size and geographical extent of outbreaks. The serotype of E. coli strains has traditionally been determined using antisera raised against the >180 different O- (somatic) and 53 H- (flagellar) antigens. However, there are many problems associated with serotyping, including: it is labor-intensive and time consuming; cross reactivity of the antisera with different serogroups occurs; antisera are available only in specialized laboratories; and many strains are non-typeable. Molecular serotyping targeting O-group-specific genes within the E. coli O-antigen gene clusters and genes that are involved in encoding for the different flagellar types offers an improved approach for determining the E. coliO- and H-groups. Furthermore, molecular serotyping can be coupled with determination of specific sets of virulence genes carried by the strain offering the possibility to determine O-group, pathotype, and the pathogenic potential simultaneously. Sequencing of the O-antigen gene clusters of all of the known O-groups of E. coli is now complete, and the sequences have been deposited in the GenBank database. The sequence information has revealed that some E. coli serogroups have identical sequences while others have point mutations or insertion sequences and type as different serogroups in serological reactions. There are also a number of other ambiguities in serotyping that need to be resolved. Furthermore, new E. coli O-groups are being identified. Therefore, there is an essential need to resolve these issues and to revise the E. coli serotype nomenclature based on these findings. There are emerging technologies that can potentially be applied for molecular serotyping and detection and characterization of E. coli. On a related topic, the genome sequence of thousands of E. coli strains have been deposited in GenBank, and this information is revealing unique markers such as CRISPR (clustered regularly interspaced short palindromic repeats) and virulence gene markers that could be used to identify E. coli pathotypes. Whole genome sequencing now provides the opportunity to study the role of horizontal gene transfer in the evolution and emergence of pathogenic E. coli strains. Whole genome sequencing approaches are being investigated for genotyping and outbreak investigation for regulatory and public health needs; however, there is a need for establishing bioinformatics pipelines able to handle large amounts of data as we move toward the use of genetic approaches for non-culture-based detection and characterization of E. coli and for outbreak investigations.
Author: Prof. Gary R. Acuff
Publisher: Burleigh Dodds Science Publishing
ISBN: 1786760584
Category : Technology & Engineering
Languages : en
Pages : 206
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Book Description
Reviews current research on the main pathogens affecting beef; Summarises best practice in pathogen detection and safety management on the farm; Discusses methods for ensuring safety in the food chain from slaughter to consumer handling of fresh beef
Author: Yi-Wei Tang
Publisher: Academic Press
ISBN: 0323899927
Category : Science
Languages : en
Pages : 3535
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Book Description
Molecular Medical Microbiology, Third Edition presents the latest release in what is considered to be the first book to synthesize new developments in both molecular and clinical research. The molecular age has brought about dramatic changes in medical microbiology, along with great leaps in our understanding of the mechanisms of infectious disease. This third edition is completely updated, reviewed and expanded, providing a timely and helpful update for microbiologists, students and clinicians in the era of increasing use of molecular techniques, changing epidemiology and prevalence, and increasing resistance of many pathogenic bacteria. Written by experts in the field, chapters include cutting-edge information and clinical overviews for each major bacterial group, along with the latest updates on vaccine development, molecular technology and diagnostic technology. - Completely updated and revised edition of this comprehensive and accessible reference on molecular medical microbiology - Includes full color presentations throughout - Delves into in-depth discussions on individual pathogenic bacteria in a system-oriented approach - Includes a clinical overview for each major bacterial group - Presents the latest information on vaccine development, molecular technology and diagnostic technology - Provides more than 100 chapters on all major groups of bacteria
Author:
Publisher: Academic Press
ISBN: 0128126345
Category : Technology & Engineering
Languages : en
Pages : 240
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Book Description
Advances in Food and Nutrition Research, Volume 82 provides updated knowledge on nutrients in foods and how to avoid deficiency. Topics covered in this latest release include Phenolic Compounds and its Bioavailability: In Vitro Bioactive Compounds or Health Promoters?, Health Risks of Food Oxidation, Metabolomics, Nutrition, and Potential Biomarkers of Food Quality, Intake, and Health Status, Improvement of Soybean Products Through the Response Mechanism Analysis Using Proteomic Technique, and Nanotechnology for Food Packaging and Food Quality Assessment. This resource is especially useful for those interested in the essential nutrients that should be present in the diet to reduce disease risk and optimize health. It provides the latest advances on the identification and characterization of emerging bioactive compounds with putative health benefits, as well as up-to-date information on food science, including raw materials, production, processing, distribution and consumption, with an emphasis on nutritional benefits and health effects. - Features input from contributors who have been carefully selected based on their long experience and high expertise on the subject - Provides an updated and in-depth critical discussion of the latest knowledge about nutrients in foods and how to avoid their deficiency - Provides the latest advances on the identification and characterization of emerging bioactive compounds with putative health benefit - Offers up-to-date information on food science, including raw materials, production, processing, distribution and consumption, with an emphasis on nutritional benefits and health effects
Author: Food and Agriculture Organization of the United Nations
Publisher: Food & Agriculture Org. [Author] [Author]
ISBN: 9251381712
Category : Science
Languages : en
Pages : 100
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Book Description
This publication is a building block of the Eastern Africa antimicrobial resistance (AMR) surveillance roadmap that was described in April 2019, by AMR experts from Djibouti, Eritrea, Ethiopia, Kenya, Rwanda, South Sudan, Sudan and Uganda. [Author] The roadmap is presented in chapter six of this document. [Author] The national AMR experts came together in a regional meeting organized by the Food and Agriculture Organization of the United Nations (FAO) and also attended by other national, regional and international organizations such as the World Organisation for Animal Health (WOAH), International Livestock Research Institute (ILRI), Centres for Disease Control and Prevention (CDC)-Kenya, University of Nairobi (UON), Kenya Medical Research Institute (KEMRI), World Animal Protection (WAP) and African Union-Interagency Bureau for Animal Resources (AU-IBAR). [Author] The aim of the roadmap is to set out the processes, tools and coordination that technical experts and decision-makers from within national governments in East Africa agreed should be undertaken at regional level to support development and implementation of national AMR surveillance strategies and plans. [Author]
Author:
Publisher: Academic Press
ISBN: 0124159761
Category : Science
Languages : en
Pages : 719
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Book Description
The second edition of Microbiology of Waterborne Diseases describes the diseases associated with water, their causative agents and the ways in which they gain access to water systems. The book is divided into sections covering bacteria, protozoa, and viruses. Other sections detail methods for detecting and identifying waterborne microorganisms, and the ways in which they are removed from water, including chlorine, ozone, and ultraviolet disinfection. The second edition of this handbook has been updated with information on biofilms and antimicrobial resistance. The impact of global warming and climate change phenomena on waterborne illnesses are also discussed. This book serves as an indispensable reference for public health microbiologists, water utility scientists, research water pollution microbiologists environmental health officers, consultants in communicable disease control and microbial water pollution students. Focuses on the microorganisms of most significance to public health, including E. coli, cryptosporidium, and enterovirus Highlights the basic microbiology, clinical features, survival in the environment, and gives a risk assessment for each pathogen Contains new material on antimicrobial resistance and biofilms Covers drinking water and both marine and freshwater recreational bathing waters
Author: Eduardo A. Groisman
Publisher: Academic Press
ISBN: 0080539580
Category : Medical
Languages : en
Pages : 863
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Book Description
Principles of Bacterial Pathogenesis presents a molecular perspective on a select group of bacterial pathogens by having the leaders of the field present their perspective in a clear and authoritative manner. Each chapter contains a comprehensive review devoted to a single pathogen. Several chapters include work from authors outside the pathogenesis field, providing general perspectives on the evolution, regulation, and secretion of virulence and determinants. - Explains the basic principles of bacterial pathogenesis - Covers diverse aspects integrating regulation, cellular microbiology and evolution of microbial disease of humans - Discusses current strategies for the identification of virulence determinants and the methods used by microbes to deliver virulence factors - Presents authoritative treatises of the major disease microorganisms
Author: Thomas M. Schmidt
Publisher: Academic Press
ISBN: 0128117370
Category : Science
Languages : en
Pages : 3248
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Book Description
Encyclopedia of Microbiology, Fourth Edition, Five Volume Set gathers both basic and applied dimensions in this dynamic field that includes virtually all environments on Earth. This range attracts a growing number of cross-disciplinary studies, which the encyclopedia makes available to readers from diverse educational backgrounds. The new edition builds on the solid foundation established in earlier versions, adding new material that reflects recent advances in the field. New focus areas include `Animal and Plant Microbiomes’ and ‘Global Impact of Microbes`. The thematic organization of the work allows users to focus on specific areas, e.g., for didactical purposes, while also browsing for topics in different areas. Offers an up-to-date and authoritative resource that covers the entire field of microbiology, from basic principles, to applied technologies Provides an organic overview that is useful to academic teachers and scientists from different backgrounds Includes chapters that are enriched with figures and graphs, and that can be easily consulted in isolation to find fundamental definitions and concepts
Author: Toonika Rinken
Publisher: BoD – Books on Demand
ISBN: 9535139150
Category : Medical
Languages : en
Pages : 218
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Book Description
Rapid multiplex detection of pathogens in the environment and in our food is a key factor for the prevention and effective treatment of infectious diseases. Biosensing technologies combining the high selectivity of biomolecular recognition and the sensitivity of modern signal detection platforms are a prospective option for automated analyses. They allow rapid detection of single molecules as well as cellular substances. This book, including 12 chapters from 50 authors, introduces the principles of identification of specific pathogen biomarkers along with different biosensor-based technologies applied for pathogen detection.
