Characterization of the Non-Darkening Gene in a Cranberry Bean (Phaseolus Vulgaris L.) Background PDF Download
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Author: Dana Robson
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Several common bean (Phaseolus vulgaris L.) market classes, including cranberry bean, demonstrate seed coat post-harvest darkening (PHD). PHD is associated with poor quality, lowering the overall value of the bean. A candidate MYB was sequenced in darkening (RD) and non-darkening (ND) cranberry bean lines to determine any differences between them. The RD and ND lines were identical for the candidate MYB. An F2 population of a cross between Etna (RD) and Witrood (ND) was created to study the inheritance of the PHD trait and identify its genomic location. The F2 lines were genotyped using Single Nucleotide Polymorphism (SNP) markers and used to develop a linkage map with 11 linkage groups. Multiple QTL Mapping (MQM) identified two QTL associated with colour parameter values on chromosome 10. SNP markers identified with the ND loci may be used in the future for marker assisted selection to develop ND cultivars.
Author: Dana Robson
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Several common bean (Phaseolus vulgaris L.) market classes, including cranberry bean, demonstrate seed coat post-harvest darkening (PHD). PHD is associated with poor quality, lowering the overall value of the bean. A candidate MYB was sequenced in darkening (RD) and non-darkening (ND) cranberry bean lines to determine any differences between them. The RD and ND lines were identical for the candidate MYB. An F2 population of a cross between Etna (RD) and Witrood (ND) was created to study the inheritance of the PHD trait and identify its genomic location. The F2 lines were genotyped using Single Nucleotide Polymorphism (SNP) markers and used to develop a linkage map with 11 linkage groups. Multiple QTL Mapping (MQM) identified two QTL associated with colour parameter values on chromosome 10. SNP markers identified with the ND loci may be used in the future for marker assisted selection to develop ND cultivars.
Author: Jose Freixas Coutin
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Postharvest-related seed coat darkening is a major problem in common bean (Phaseolus vulgaris L.), including cranberry bean, as these seeds are graded lower in the commercial market. Proanthocyanidins accumulate in the seed coats of dicotyledonous species, including legumes and their oxidation to quinones promotes seed coat darkening. The upregulated expression of genes encoding late steps in proanthocyanidin biosynthesis (e.g., ANTHOCYANIDIN REDUCTASE, ANR), are associated with seed coat darkening in Medicago truncatula and Arabidopsis thaliana. In this thesis, total proanthocyanidin levels in seed coats increased with seed maturation in a darkening-susceptible cranberry bean recombinant inbred line (RIL), whereas these compounds were undetectable in non-darkening RIL seed coats. RNA sequencing was implemented to investigate transcript changes in darkening and non-darkening RIL seed coats as a function of bean development. The transcript levels for 1336 genes were varied between darkening and non-darkening RIL seed coats at one or more stages of seed development. A model clustering analysis revealed the differentially expressed genes formed 14 gene clusters based on their mode of expression. Structural and regulatory genes of the proanthocyanidin biosynthesis pathway were prevalent in cluster 2 and upregulated in seed coats of the darkening RIL. This included myeloblastosis proto-oncogene (MYB) transcription factors with phylogenetic similarity to proanthocyanidin activating MYBs from other plant species. Moreover, regions upstream of the transcription start site in cluster 2 genes were enriched in MYB and basic helix-loop-helix consensus binding sites as revealed by a transcription factor binding site enrichment analysis. A principal component analysis revealed that transcript abundance of two genes of unknown function and three proanthocyanidin biosynthesis genes, FLAVANONE 3-HYDROXYLASE 1, DIHYDROFLAVONOL 4-REDUCTASE 1 and PvANR1 were highly associated with seed coat proanthocyanidin accumulation in the darkening cranberry bean RIL. Biochemical characterization of a recombinant PvANR1 was performed following the removal of the hexahistidine-tag. PvANR1 catalyzed the NADPH-dependent reduction of cyanidin to catechin and epicatechin in vitro; moreover, high cyanidin concentrations inhibited the formation of both of these products. This study provides biochemical and molecular information for traditional breeding strategies aimed at preserving the aesthetic quality of edible dry beans during postharvest storage.
Author: Viviana Lorena Becerra Velasquez
Publisher:
ISBN:
Category :
Languages : en
Pages : 168
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Book Description
Author: Mohammad Erfatpour
Publisher:
ISBN:
Category :
Languages : en
Pages :
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Book Description
Seed coat colour is one of the main determinants of seed quality in commercial market classes of dry bean (P. vulgaris L.), including pinto bean. The beige background of the conventional pinto bean seed coat turns brown with age through a process known as postharvest seed coat darkening (PSCD). Beans with darkened seed coat are discounted in the market because they are perceived to be old, hard-to-cook beans. Seed coat darkening (SCD) is attributed to oxidation of proanthocyanidins (PAs) in the seed coat. Breeding for beans with reduced risk of PSCD has been given more attention in recent years because they are appealing to consumers and command higher prices in the market. To achieve this objective, it is important to understand the genetic control of the SCD trait and its relationship with other traits. J is a Mendelian genetic locus known to be responsible for SCD trait in dry beans in which the recessive allele produces a non-darkening (ND) seed coat phenotype. The main objectives of this thesis were to identify quantitative trait loci (QTL) and a gene associated with PSCD in dry beans and understand its function in promoting SCD. A mapping population consisting of 128 F5 recombinant inbred lines (RILs) derived from a cross between a ND cranberry-like bean 'Wit-rood boontje' and a slow-darkening pinto bean '1533-15' was genotyped with an Illumina BARCBEAN6K_3 BeadChip and F5:6 seeds were characterized for seed coat phenotype. A major QTL was identified on chromosome Pv10 which explained 48.1% of the phenotypic variation for seed coat darkening. Amplicon sequencing of 21 candidate genes underlying the QTL revealed a single nucleotide deletion (c.703delG) in the candidate gene Phvul.010G130600 in ND RILs. In silico analysis indicated that Phvul.010G130600 encodes a protein with strong amino acid sequence identity (70%) to a R2R3-MYB-type transcription factor MtPAR, which regulates PA biosynthesis in Medicago truncatula seed coat tissue. The ND trait was correlated with reduced grain yield; however, it is possible to develop high yielding lines. In comparison with the darkening genotypes, the ND genotypes were perceived as sweeter, and had softer and smoother texture and shorter cooking times.
Author: Muhammad Siddiq
Publisher: John Wiley & Sons
ISBN: 1118448286
Category : Technology & Engineering
Languages : en
Pages : 452
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Book Description
The common beans and pulses are diverse food resources of high nutritional value (protein, energy, fiber and vitamins and minerals) with broad social acceptance. These legume crops demonstrate global adaptability, genotypic and phenotypic diversity, and multiple means of preparation and dietary use. Beans and pulses are produced in regions as diverse as Latin America, Africa, Asia, and North America, and on a scale similar to some other crops, such as wheat, corn, rice and soybeans. Numerous factors influence utilization, including bean type and cultivar selection, cropping environment and systems, storage conditions and handling infrastructure, processing and final product preparation. Nutrient content and bio-availability are dramatically influenced by these conditions. In recent years, beans and pulses have been cited for imparting specific positive health potentiating responses, such as hypocholesteremic response, mitigation of diabetes and colonic cancer, and weight control. Enhanced dry bean utilization focused on improved dietary health is an opportunity within both subsistent and developed populations. This book provides a contemporary source of information that brings together current knowledge and practices in the value chain of beans/pulses production, processing, and nutrition. It provides in-depth coverage of a wide variety of pertinent topics including: breeding, postharvest technologies, composition, processing technologies, food safety, quality, nutrition, and significance in human health. An experienced team of over 25 contributors from North America, Asia, and Africa has written 15 chapters, divided into three sections: Overview, production and postharvest technologies of beans and pulses Composition, value-added processing and quality Culinology, nutrition, and significance in human health Contributors come from a field of diverse disciplines, including crop sciences, food science and technology, food biochemistry, food engineering, nutritional sciences, and culinology. Dry Beans and Pulses Production, Processing and Nutrition is an essential resource for scientists, processors and nutritionists, whatever the work setting.
Author: Fereidoon Shahidi
Publisher:
ISBN:
Category : Cooking
Languages : en
Pages : 328
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Book Description
The book reviewed and gave new research discovery of the chemistry, analysis and health effects such as antioxidative, cancer preventive and anti-inflammatory properties of phenolic compounds in foods and natural health products.
Author: Ram J. Singh
Publisher: CRC Press
ISBN: 0203489284
Category : Science
Languages : en
Pages : 392
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Book Description
The first book in this new series discusses grain legumes, which rank only second to cereals in supplying calories and protein to the world's population. With each chapter written by an internationally renowned scientist, the book reviews the role of alien germplasm for the domestication of each major legume crop. Discussion for each crop covers or
Author: Jules Janick
Publisher: John Wiley & Sons
ISBN: 0471997986
Category : Technology & Engineering
Languages : en
Pages : 355
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Book Description
Plant Breeding Reviews presents state-of-the-art reviews on plant genetics and the breeding of all types of crops by both traditional means and molecular methods. Many of the crops widely grown today stem from a very narrow genetic base; understanding and preserving crop genetic resources is vital to the security of food systems worldwide. The emphasis of the series is on methodology, a fundamental understanding of crop genetics, and applications to major crops. It is a serial title that appears in the form of one or two volumes per year.
Author: Howard F. Schwartz
Publisher: CIAT
ISBN: 9789589183045
Category : Technology & Engineering
Languages : en
Pages : 750
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Book Description
The first section reviews trends of bean production and constraints in Latin America and Africa. The second section covers fungal diseases. The third section, bacterial diseases. The fourth section, viral and mycoplasma diseases. The fifth section, insect pests. The last section, other bean production constraints, that is, nutritional disorders, nematodes, seed pathology, and additional problems.
Author: Marcelino Pérez de la Vega
Publisher: Springer
ISBN: 3319635263
Category : Science
Languages : en
Pages : 304
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Book Description
This book provides insights into the genetics and the latest advances in genomics research on the common bean, offering a timely overview of topics that are pertinent for future developments in legume genomics. The common bean (Phaseolus vulgaris L.) is the most important grain legume crop for food consumption worldwide, as well as a model for legume research, and the availability of the genome sequence has completely changed the paradigm of the ongoing research on the species. Key topics covered include the numerous genetic and genomic resources, available tools, the identified genes and quantitative trait locus (QTL) identified, and there is a particular emphasis on domestication. It is a valuable resource for students and researchers interested in the genetics and genomics of the common bean and legumes in general.
