Characterization of the DNA Polymerase Delta Multiprotein Complex by Two-dimensional Gel Electrophoresis and Affinity Chromatography PDF Download
Are you looking for read ebook online? Search for your book and save it on your Kindle device, PC, phones or tablets. Download Characterization of the DNA Polymerase Delta Multiprotein Complex by Two-dimensional Gel Electrophoresis and Affinity Chromatography PDF full book. Access full book title Characterization of the DNA Polymerase Delta Multiprotein Complex by Two-dimensional Gel Electrophoresis and Affinity Chromatography by Cindy M. Jaime. Download full books in PDF and EPUB format.
Author: Cindy M. Jaime
Publisher:
ISBN:
Category :
Languages : en
Pages : 69
Get Book Here
Book Description
Author: Cindy M. Jaime
Publisher:
ISBN:
Category :
Languages : en
Pages : 69
Get Book Here
Book Description
Author: Ulrich Hübscher
Publisher: World Scientific
ISBN: 9814299170
Category : Medical
Languages : en
Pages : 338
Get Book Here
Book Description
Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex processes that maintain genetic integrity. Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, genome sequencing, nucleic acids-based diagnostics and in techniques to analyze ancient and otherwise damaged DNA. Moreover, some diseases are related to DNA polymerase defects, and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes and Hepatitis B and C infections. We have recently witnessed the discovery of an abundance of novel DNA polymerases in viruses, bacteria, archaea and eukaryotes with specialized properties whose physiological functions are only beginning to be understood. This book summarizes the current knowledge of these fascinating enzymes. It is intended for a wide audience from basic scientists, to diagnostic laboratories and to clinicians who seek a better understanding of these fascinating enzymes.
Author: Nayef Ali Mazloum
Publisher:
ISBN:
Category :
Languages : en
Pages : 162
Get Book Here
Book Description
Author: Andrew J. Link
Publisher: Springer Science & Business Media
ISBN: 1592595847
Category : Science
Languages : en
Pages : 585
Get Book Here
Book Description
With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biological problems. The contri- tors include many of the leaders in the fields of biochemistry and analytical chemistry who were instrumental in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spectromet- based protein identification methodologies. This book is intended as a benchtop manual and guide both for novices to 2-D gels and for those aficionados who wish to try the newer techniques. Any group using protein biochemistry—especially in the fields of molecular biology, biochemistry, microbiology, and cell biology—should find this book eminently useful. 2-D Proteome Analysis Protocols takes the researcher through the c- plete process of working with 2-D protein gels from making the protein - tract to finally identifying the proteins of interest. It includes protocols for generating 2-D protein extracts from most of the standard model organisms, including bacteria, yeast, nematode, Drosophila, plants, mouse, and human.
![DNA polymerase [delta] PDF](https://books.google.com/books/content/images/frontcover/QwPuMAAACAAJ?fife=w80-h100)
Author: Robert Jacques Hindges
Publisher:
ISBN:
Category :
Languages : de
Pages : 99
Get Book Here
Book Description
Author: Zvi Kelman
Publisher: Frontiers Media SA
ISBN: 2889194558
Category : Biotechnology
Languages : en
Pages : 147
Get Book Here
Book Description
DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology. For example, DNA polymerases have been engineered for increased speed and fidelity in PCR while lowering amplification sequence bias. Inhibitor resistant DNA polymerase variants enable PCR directly from tissue (i.e. blood). Design of DNA polymerases that efficiently incorporate modified nucleotide have been critical for development of next generation DNA sequencing, synthetic biology and other labeling and detection technologies. The Frontiers in Microbiology Research Topic on DNA polymerases in Biotechnology aims to capture current research on DNA polymerases and their use in emerging technologies.
Author: Anton Posch
Publisher: Humana Press
ISBN: 9781588297228
Category : Science
Languages : en
Pages : 490
Get Book Here
Book Description
This book presents broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and two-dimensional electrophoresis (2-DE) in particular. With its unique capacity to resolve thousands of proteins in a single run, 2-DE is still a fundamental research tool for nearly all protein-related scientific projects.
Author: Giovanni Maga
Publisher: World Scientific
ISBN: 9813226420
Category : Science
Languages : en
Pages : 398
Get Book Here
Book Description
Maintenance of the information embedded in the genomic DNA sequence is essential for life. DNA polymerases play pivotal roles in the complex processes that maintain genetic integrity. Besides their tasks in vivo, DNA polymerases are the workhorses in numerous biotechnology applications such as the polymerase chain reaction (PCR), cDNA cloning, next generation sequencing, nucleic acids based diagnostics and in techniques to analyze ancient and otherwise damaged DNA (e.g. for forensic applications). Moreover, some diseases are related to DNA polymerase defects and chemotherapy through inhibition of DNA polymerases is used to fight HIV, Herpes and Hepatitis B and C infections. This book focuses on (i) biology of DNA polymerases, (ii) medical aspects of DNA polymerases and (iii) biotechnological applications of DNA polymerases. It is intended for a wide audience from basic scientists, to diagnostic laboratories, to companies and to clinicians, who seek a better understanding and the practical use of these fascinating enzymes.
![The Purification and Characterization of HeLa Cytosolic DNA Polymerase [alpha] and Two Stimulatory Proteins PDF](https://books.google.com/books/content/images/frontcover/66zVAAAAMAAJ?fife=w80-h100)
Author: Jack H. Wang
Publisher:
ISBN:
Category : DNA polymerases
Languages : en
Pages : 378
Get Book Here
Book Description
![Biochemical Characterization of DNA Polymerase [delta] and Cloning of Its CDNA from the Glycine Max Cell Line SB-M PDF](https://books.google.com/books/content/images/frontcover/HdZbNwAACAAJ?fife=w80-h100)
Author: Jeannie T. B. Collins
Publisher:
ISBN:
Category : Cloning
Languages : en
Pages : 226
Get Book Here
Book Description
