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Author: Anthony B. Chen
Publisher: Springer Science & Business Media
ISBN: 3322830217
Category : Technology & Engineering
Languages : en
Pages : 103
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Book Description
The goal of this book is to show recent developments in the CE analysis of protein pharmaceuticals. It is devoted completely to practical concerns to strengthen the use of CE within the biotechnology industry, highlighting the uses of CE in various areas of product development including formulation studies, process development, product characterization and validated lot release and stability testing.
Author: Anthony B. Chen
Publisher: Springer Science & Business Media
ISBN: 3322830217
Category : Technology & Engineering
Languages : en
Pages : 103
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Book Description
The goal of this book is to show recent developments in the CE analysis of protein pharmaceuticals. It is devoted completely to practical concerns to strengthen the use of CE within the biotechnology industry, highlighting the uses of CE in various areas of product development including formulation studies, process development, product characterization and validated lot release and stability testing.
Author: Mark A. Strege
Publisher: Springer Science & Business Media
ISBN: 159259798X
Category : Science
Languages : en
Pages : 340
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Book Description
Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.
Author: Nguyet Thuy Tran
Publisher:
ISBN: 9781493940141
Category : Capillary electrophoresis
Languages : en
Pages : 234
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Book Description
"This book provides a comprehensive survey of recent developments and applications of high performance capillary electrophoresis in the field of protein and peptide analysis with a distinct focus on the analysis of intact proteins. With practical detail, the contents cover different modes of capillary electrophoresis (CE) useful for protein and peptide analysis, CZE, CIEF, ACE, CGE, and different types of application such as the quality control of therapeutic proteins and monoclonal antibodies, clinical analyses of chemokines in tissues, qualitative and quantitative analysis of vaccine proteins, and determination of binding constants in complexes involving peptides or proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and exhaustive, Capillary Electrophoresis of Proteins and Peptides: Methods and Protocols serves both beginners and experts with a collection of the current and most active topics in this vital field of study."--OCLC.
Author: William S. Hancock
Publisher: CRC Press
ISBN: 9780849378225
Category : Medical
Languages : en
Pages : 280
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Book Description
This text is devoted to the characterization of recombinant DNA-derived proteins by peptide mapping. It describes new technological procedures including capillary electrophoresis, analysis of glycopeptides and the use of electrospray and matrix-assisted laser desorption mass spectrometry. The book presents practical procedures for preparing a protein sample, the enzyme digestion, choice of separation method and procedures for the structural analysis of the separated species. Many figures of peptide maps illustrate typical results. Tables of summary information about digestion, separation conditions, and analyses of important protein samples are also presented.
Author: Robert A. Copeland
Publisher: Springer Science & Business Media
ISBN: 1475715056
Category : Science
Languages : en
Pages : 238
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Book Description
As protein science continues to become an increasingly important aspect of academic and commercial sciences and technology, the need has arisen for a ready source of laboratory protocols for the analysis and evaluation of these biological polymers. Methods for Protein Analysis presents the methods most relevant to the generalist bench scientist working with proteins. A concise yet thorough summary, it covers laboratory methods that can be reasonably performed in a standard protein laboratory, without specialized equipment or expertise. Taking a how to approach, this book examines the techniques used to answer common protein analytical questions and describes methods useful in daily laboratory work. Methods for Protein Analysis is the ideal reference for protein laboratories in academic, government and industrial settings. It is an essential benchtop manual for first-year graduate students beginning their laboratory experience as well as for chemists, biochemists, and molecular biologists in the pharmaceutical, biotechnological, food and specialty chemical industries, and for analysts concerned with the purity and structural integrity of protein. Featuring illustrations and a convenient spiral binding, this guide offers a glossary of common abbreviations and a list of suppliers for protein science.
Author: Paul T. Matsudaira
Publisher: Elsevier
ISBN: 0080924611
Category : Science
Languages : en
Pages : 205
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Book Description
Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Mass spectrophotometric characterization of proteins and peptides N-terminal sequencing of proteins with blocked N termini Internal amino acid sequence analysis after protease digestion in-gel and on-blot Improved microscale peptide purification methods Computer analysis of protein sequences New protocols tested and refined through everyday use in authors' laboratories Updated reference chapter covering all aspects of protein microsequencing
Author: Andras Guttman
Publisher: Newnes
ISBN: 0080931359
Category : Science
Languages : en
Pages : 391
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Book Description
Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Author: Tim Wehr
Publisher: CRC Press
ISBN: 1482289857
Category : Science
Languages : en
Pages : 301
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Book Description
"Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."
Author: Stephen Wren
Publisher: Springer Science & Business Media
ISBN: 3322831418
Category : Technology & Engineering
Languages : en
Pages : 96
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Book Description
This book gives a comprehensive overview on the principles of physical and mathematical modelling of enantiomer separation by CE. Method development strategies are shown and cyclodextrins as a popular group of chiral selectors as well as some other selectors used as buffer additives for enantiomer separation in free solution are described.
Author: David Garfin
Publisher: Elsevier
ISBN: 0080533620
Category : Science
Languages : en
Pages : 358
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Book Description
Isoelectric focusing (IEF) is a high-resolution, stand-alone technique that can be used as an analytical method or tool for protein purification. The only current book on the market, the Handbook of Isoelectric Focusing and Proteomics is the ideal 'one-stop' source for germane information in this discipline. This highly practical book also contains chapters on alternative methods that may pave the way in the search for efficient techniques for fractionating and purifying proteins. Complete with the history of IEF focusing to authors' insights and practical tips, this book is a must for anyone working in proteomics. * Is the only current book available on the subject * Includes author insights and practical tips* Is an ideal single source for students and researchers working in proteomics
