Biomolecular Imaging at High Spatial and Temporal Resolution in Vitro and in Vivo PDF Download
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Author: Thomas H. Sharp
Publisher:
ISBN: 9783319021607
Category :
Languages : en
Pages : 170
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Book Description
Author: Thomas H. Sharp
Publisher:
ISBN: 9783319021607
Category :
Languages : en
Pages : 170
Get Book Here
Book Description
Author: Thomas Harry Sharp
Publisher: Springer
ISBN: 9783319350233
Category : Science
Languages : en
Pages : 0
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Book Description
As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his Ph.D., Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.
Author: Thomas Harry Sharp
Publisher: Springer Science & Business Media
ISBN: 3319021591
Category : Science
Languages : en
Pages : 161
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Book Description
As part of a collaboration between two different groups in chemistry and biochemistry, Thom Sharp presents here his thesis work on the development of new methods for cryoelectron microscopy. Throughout his Ph.D., Thom had to master a whole range of techniques including modelling, molecular biology and microscopy. Using these skills to tackle an outstanding problem, the pursuit of high-resolution structures of peptide-based materials, Thom highlights in this thesis his newly developed methods for analysing and processing this particular type of electron microscopy data. This thesis gives the first molecular description of a de-novo designed peptide-based material. In general, this research will have a huge impact on the peptide assembly field, and also in electron microscopy as it introduces new methods and approaches, all of which are Thom's inventions and are described in this thesis.
Author: Christopher P. Toseland
Publisher: Springer
ISBN: 3034808569
Category : Science
Languages : en
Pages : 306
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Book Description
This book focuses on the application of fluorescence to study motor proteins (myosins, kinesins, DNA helicases and RNA polymerases). It is intended for a large community of biochemists, biophysicists and cell biologists who study a diverse collection of motor proteins. It can be used by researchers to gain an insight into their first experiments, or by experienced researchers who are looking to expand their research to new areas. Each chapter provides valuable advice for executing the experiments, along with detailed background knowledge in order to develop own experiments.
Author: Kam-Seng Lau
Publisher: Open Dissertation Press
ISBN: 9781361011584
Category : Technology & Engineering
Languages : en
Pages : 198
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Book Description
This dissertation, "Quantitative Time-stretch Imaging -- Towards Big-data Bioassay" by Kam-seng, Lau, 劉金成, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: The unrelenting growth in the field of optical bioimaging over the past half-century has been leading to a ubiquitously wide range of applications, from in-vitro single-cell analysis to large-scale in-vivo imaging. In light of the complexity and heterogeneity of the biological systems (from molecular, cellular to even the whole-organism level), development of new optical imaging/microscopy technologies should be focused on improving spatial resolution, temporal resolution and enlarging the quantitative image information content. To this end, this thesis will encompass an entirely new imaging modality that can offer ultra-high temporal resolution in a continuous mode, plus the high-information content obtained from the individual single-cell optical images. In the first part, I will introduce this ultrafast, high-throughput optical imaging modality dubbed as time-stretch imaging. This technology allows ultra-fast, high-throughput imaging capability (up to 26 million line-scans per second). Applying this technology to biophotonics applications, we are able to demonstrate time-stretch microscopy with less optical absorption and better diffraction-limited resolution ( 1.5 m). This ultrafast imaging technique is particularly useful for high-throughput and high-accuracy cell screening, such as imaging flow cytometry. In the second part, the development of ordinary time-stretch microscopy will be introduced, including interferometric time-stretch (iTS) microscopy, asymmetric-detection time-stretchoptical microscopy (ATOM, second generation of time-stretch microscopy) and quantitative-phase ATOM (Q-ATOM). ATOM offers dual-contrast images for individual single-cell images, especially useful for unstained live-cell imaging, such that original cell content can be restored. In addition, iTS microscopy and Q-ATOM allows capture of additional quantitative phase information, which can be further derived to obtain cell-dependent quantitative single-cell's biophysical parameters and characteristics. I will also introduce the methods of cell screening and classifications down to single-cell precision based on both the intensity and phase images obtained. With these unique and promising features, time-stretch imaging can open a new paradigm of quantitative bio-assays and in general enable the concept of data-driven biomedicine. Subjects: Imaging systems in biology
Author: Margarida Barroso
Publisher: CRC Press
ISBN: 1351704494
Category : Science
Languages : en
Pages : 444
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Book Description
This book offers an overview of imaging techniques used to investigate cells and tissue in their native environment. It covers the range of imaging approaches used, as well as the application of those techniques to the study of biological processes in cells and whole tissues within living organisms.
Author:
Publisher: Academic Press
ISBN: 012386951X
Category : Science
Languages : en
Pages : 449
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Book Description
One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors. Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well as for applications in biomedicine, drug discovery and biotechnology. These tools have been designed and engineered thanks to the combined efforts of chemists and biologists over the last decade, and developed hand in hand together with imaging technologies. This volume will convey the many exciting developments the field of fluorescent biosensors and reporters has witnessed over the recent years, from concepts to applications, including chapters on the chemistry of fluorescent probes, on technologies for monitoring protein/protein interactions and technologies for imaging biosensors in cultured cells and in vivo. Other chapters are devoted to specific examples of genetically-encoded reporters, or to protein and peptide biosensors, together with examples illustrating their application to cellular and in vivo imaging, biomedical applications, drug discovery and high throughput screening. - Contributions from leading authorities - Informs and updates on all the latest developments in the field
Author: National Research Council
Publisher: National Academies Press
ISBN: 030916463X
Category : Science
Languages : en
Pages : 222
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Book Description
Scientists and engineers have long relied on the power of imaging techniques to help see objects invisible to the naked eye, and thus, to advance scientific knowledge. These experts are constantly pushing the limits of technology in pursuit of chemical imagingâ€"the ability to visualize molecular structures and chemical composition in time and space as actual events unfoldâ€"from the smallest dimension of a biological system to the widest expanse of a distant galaxy. Chemical imaging has a variety of applications for almost every facet of our daily lives, ranging from medical diagnosis and treatment to the study and design of material properties in new products. In addition to highlighting advances in chemical imaging that could have the greatest impact on critical problems in science and technology, Visualizing Chemistry reviews the current state of chemical imaging technology, identifies promising future developments and their applications, and suggests a research and educational agenda to enable breakthrough improvements.
Author: Bernhard Schaller
Publisher: BoD – Books on Demand
ISBN: 9535103598
Category : Medical
Languages : en
Pages : 404
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Book Description
The present book gives an exceptional overview of molecular imaging. Practical approach represents the red thread through the whole book, covering at the same time detailed background information that goes very deep into molecular as well as cellular level. Ideas how molecular imaging will develop in the near future present a special delicacy. This should be of special interest as the contributors are members of leading research groups from all over the world.
Author:
Publisher:
ISBN:
Category :
Languages : en
Pages : 8
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Book Description
Single-molecule fluorescence microscopy is a powerful approach to observe biomolecular interactions with high spatial and temporal resolution. Detecting fluorescent signals from individual, labeled proteins above high levels of background fluorescence remains challenging, however. For this reason, the concentrations of labeled proteins in in vitro assays are often kept low compared to their in vivo concentrations. Here, we present a new fluorescence imaging technique by which single fluorescent molecules can be observed in real time at high, physiologically relevant concentrations. The technique requires a protein and its macromolecular substrate to be labeled each with a different fluorophore. Then, making use of short-distance energy-transfer mechanisms, the fluorescence from only those proteins bound to their substrate are selectively activated. This approach is demonstrated by labeling a DNA substrate with an intercalating stain, exciting the stain, and using energy transfer from the stain to activate the fluorescence of only those labeled DNA-binding proteins bound to the DNA. Such an experimental design allowed us to observe the sequence-independent interaction of Cy5-labeled interferon-inducible protein 16 (IFI16) with DNA and the sliding via one-dimensional diffusion of Cy5-labeled adenovirus protease (pVIc-AVP) on DNA in the presence of a background of hundreds of nM Cy5 fluorophore.
