Biochemical and Genetic Characterization of Mot1 and Its Interaction with Components of the Basal Transcription Complex in Saccharomyces Cerevisiae PDF Download
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Author: Karin Elisabeth Hansen
Publisher:
ISBN:
Category :
Languages : en
Pages : 330
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Book Description
Author: Karin Elisabeth Hansen
Publisher:
ISBN:
Category :
Languages : en
Pages : 330
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Book Description
Author: Karin Elisabeth Hansen
Publisher:
ISBN:
Category :
Languages : en
Pages : 147
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Book Description
Author: Joanne Irene Adamkewicz
Publisher:
ISBN:
Category :
Languages : en
Pages : 338
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Author:
Publisher:
ISBN:
Category : Dissertations, Academic
Languages : en
Pages : 946
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Author:
Publisher:
ISBN:
Category : Dissertation abstracts
Languages : en
Pages : 784
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Author: Robert Brambl
Publisher: Springer Science & Business Media
ISBN: 3662103672
Category : Science
Languages : en
Pages : 451
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Book Description
The Series The fungi represent a heterogeneous assemblage of eukaryotic microorganisms and have become favored organisms for research at the cellular and molecular level. Such research involvement has been stimulated by interest in the biotechnological application of fungi in processes related to industry, agriculture and ecology. Considering both yeasts and mycelial fungi, The Mycota highlights developments in both basic and applied research and presents an overview of fungal systematics and cell structure. Foremost authorities in research on mycology have been assembled to edit and contribute to the volumes. This Volume The third volume includes: Membrane Systems and Transport, Responses to Physical Stress, Transcription, Chromosome Replication, Metabolic Pathways and Regulation.
Author:
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ISBN:
Category : Medicine
Languages : en
Pages : 1828
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Author: Joseph Locker
Publisher: Garland Science
ISBN: 1135323631
Category : Science
Languages : en
Pages : 345
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Book Description
This book focuses on the DNA-binding transcription factors and the proteins with which they directly interact. It examines the regulatory systems that modulate gene expression in all cells and the more specialized systems that regulate localized gene expression throughout the mammalian organism.
Author: Samantha A. Pillon
Publisher:
ISBN:
Category :
Languages : en
Pages : 206
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Book Description
The regulation of transcription is an important cellular function because it is the first step in gene regulation. In Saccharomyces cerevisiae, two protein complexes, SAGA and NuA4, act as regulators of transcription. A common protein shared between these two complexes, called Tra1, regulates transcriptional activation through its interaction with gene specific transcriptional activators. Tra1 is a member of the PIKK family of proteins, which are characterized by FAT, PI3K and FATC domains. The FATC domain encompasses the terminal 33-35 residues of the protein. Two mutations within the FATC domain, tra1-L3733A and tra1-F3744A, result in slow growth under stress conditions. Partially dominant mutations in the gene encoding Tti2, a 421 residues component of the TTT chaperone complex, suppressed these phenotypes. My goal was to further characterize the role of the FATC domain of Tra1 by determining which residues are important for function, and characterize how these relate to the TRA1-TTI2 interaction. I created alleles of tra1 which convert the terminal residue to serine (tra1-F3744S) or arginine (tra1-F3744R), an allele with the two terminal residues inverted (tra1-WF-FW), and alleles which resulted in deletions of one or two residues (tra1-1and tra1-2). tra1-WF-FW supported cell viability, whereas tra1-F3744S supported cell viability in the presence of the tti2- F328S suppressor. Slow growth at 37°C resulting from Tra1-WF-FW was also suppressed by tti2. Tra1-F3744S grew slowly at 37°C and on 6% ethanol in the presence of the suppressor. tra1-F3744R, tra1-1, and tra1-2 would not support viability in the presence or absence of the suppressor. To better understand the structure and function of Tti2, truncation mutations were created to identify essential regions of Tti2. These truncation mutations were assessed by analyzing cell viability, protein expression levels, and interaction with Tti1 and Tel2, two other members of the TTT complex. Only Tti2-53-421 (containing residues 53-421) supported viability and retained its ability to interact with Tel2 and Tti1 at near wildtype levels. Tti2-53-238 and Tti2-1-238 interact with Tel2 but do not support viability. All of the other mutations did not support viability and showed minimal binding affinity to Tti1 and Tel2 A terminal mutation of another PIKK family member, Mec1 (mec1-W2368A) also results in slow growth at 37°C. Interestingly, the tti2-F328S suppressor does not suppress the mec1- W2368A phenotype. Using bioinformatics approaches, I identified rpn3-L140P, encoding a component of the proteasomal cap, as a suppressor of mec1-W2368A.
Author: Fumiaki Uchiumi
Publisher: BoD – Books on Demand
ISBN: 9535138553
Category : Medical
Languages : en
Pages : 336
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Book Description
"Central dogma" was presented by Dr. Francis Crick 60 years ago. The information of nucleotide sequences on DNAs is transcribed into RNAs by RNA polymerases. We learned the mechanisms of how transcription determines function of proteins and behaviour of cells and even how it brings appearances of organisms. This book is intended for scientists and medical researchers especially who are interested in the relationships between transcription and human diseases. This volume consists of an introductory chapter and 14 chapters, divided into 4 parts. Each chapter is written by experts in the basic scientific field. A collection of articles presented by active and laboratory-based investigators provides recent advances and progresses in the field of transcriptional regulation in mammalian cells.
