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Author: P. D. Bridge
Publisher: CABI
ISBN: 0851992331
Category : Science
Languages : en
Pages : 384
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Book Description
Since the initial report of the amplification of specific DNA fragments using the polymerase chain reaction (PCR) in 1985, this technique has revolutionized molecular biology. It enables the production of large quantities of DNA from minute amounts of sample material, which can then be readily analyzed. This facility has had an enormous influence on the way both fundamental and diagnostic questions are approached and its use is now considered essential for molecular work in all branches of biology. The purpose of this book is to highlight the wide-ranging applications of PCR in pure and applied mycology and to increase understanding of its potential benefits. After a brief overview, a group of internationally-renowned mycologists give definitive descriptions of the use of PCR in their own specialized fields. These include fungal gene expression and cloning, taxonomy and speciation, fungal mycobionts, mycorrhizal fungi, entomopathogenic fungi, mycotoxin-producing fungi, diagnosis of fungal infections in animals, seed-borne diseases, fungal/plant interactions and applications with industrially-important fungi. Finally, potential future directions for PCR work in mycology are discussed.
Author: P. D. Bridge
Publisher: CABI
ISBN: 0851992331
Category : Science
Languages : en
Pages : 384
Get Book Here
Book Description
Since the initial report of the amplification of specific DNA fragments using the polymerase chain reaction (PCR) in 1985, this technique has revolutionized molecular biology. It enables the production of large quantities of DNA from minute amounts of sample material, which can then be readily analyzed. This facility has had an enormous influence on the way both fundamental and diagnostic questions are approached and its use is now considered essential for molecular work in all branches of biology. The purpose of this book is to highlight the wide-ranging applications of PCR in pure and applied mycology and to increase understanding of its potential benefits. After a brief overview, a group of internationally-renowned mycologists give definitive descriptions of the use of PCR in their own specialized fields. These include fungal gene expression and cloning, taxonomy and speciation, fungal mycobionts, mycorrhizal fungi, entomopathogenic fungi, mycotoxin-producing fungi, diagnosis of fungal infections in animals, seed-borne diseases, fungal/plant interactions and applications with industrially-important fungi. Finally, potential future directions for PCR work in mycology are discussed.
Author: Bhim Pratap Singh
Publisher: Springer
ISBN: 3319341065
Category : Science
Languages : en
Pages : 370
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Book Description
The Kingdom fungi encompass a massive diversity of taxa with wide-ranging ecologies, life cycles, and morphologies ranging from unicellular aquatic chytrids to large mushrooms. Before molecular methods came in existence, taxonomists considered this Kingdom to be a member of the plant kingdom due to certain life styles like immobility and growth habitats. Molecular markers (also known as DNA markers), facilitated a better alternative method over traditional morphological methods, employed for the identification, characterization, and to understand the evolution of fungi. The morphological methods used for identification are mainly dependent on spore color or microscopic features whereas molecular markers are based on DNA polymorphism in the genomic organization. Phylogenetic studies reported in last decade, based on molecular markers, have reshaped the classification system of Kingdom fungi, which divided into one subkingdom, seven phyla, and ten subphyla. Recent advances in molecular mycology have opened the way for researchers to identify and characterize novel fungal species from unique environments. Mycology is concerned with the systematic study of fungi, including their genetic and biochemical properties, their use to humans as a source of medicine and food, as well as their dangers, such as poisoning and infections. In the 21st century with the development of DNA sequencing technologies and phylogenetic analysis based on molecular markers, new insights into fungal taxonomy were provided. This book contains a thorough discussion of molecular characterization and detection of different groups of fungi by using PCR-based markers and provides a comprehensive view of the applications and uses of different molecular markers in molecular mycology. It also addresses the recent molecular markers employed to solve the problems of identification and discusses current approaches used in molecular characterization and detection of fungi.
Author: Youssuf Gherbawy
Publisher: Springer Science & Business Media
ISBN: 3642050425
Category : Science
Languages : en
Pages : 511
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Book Description
Fungi enjoy great popularity in pharmaceutical, agricultural, and biotechnological applications. Recent advances in the decipherment of whole fungal genomes promise an acceleration of these trends. This timely book links scientists from different parts of the world who are interested in the molecular identification of fungi combined with the exploration of the fungal biodiversity in different ecosystems. It provides a compendium for scientists who rely on a rapid and reliable detection of fungal specimens in environmental as well as clinical resources in order to ensure the benefit of industrial and clinical applications. Chapters focus on the opportunities and limits of the molecular marker-mediated identification of fungi. Various methods, procedures and strategies are outlined. Furthermore, the book offers an update of the current progress in the development of fungal molecular techniques, and draws attention to potential and associated problems, as well as integrating theory and practice.
Author: Vijai Kumar Gupta
Publisher: Springer Science & Business Media
ISBN: 1461423562
Category : Science
Languages : en
Pages : 608
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Book Description
Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals. Coming Soon in the Fungal Biology series: Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2 van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi Schmoll, Dattenbock / Gene Expression Systems in Fungi Dahms / Advanced Microscopy in Mycology
Author: Christopher C. Kibbler
Publisher: Oxford University Press
ISBN: 0198755384
Category : Medical
Languages : en
Pages : 401
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Book Description
The Oxford Textbook of Medical Mycology is a comprehensive reference text which brings together the science and medicine of human fungal disease. Written by a leading group of international authors to bring a global expertise, it is divided into sections that deal with the principles of mycology, the organisms, a systems based approach to management, fungal disease in specific patient groups, diagnosis, and treatment. The detailed clinical chapters take account of recent international guidelines on the management of fungal disease. With chapters covering recent developments in taxonomy, fungal genetics and other 'omics', epidemiology, pathogenesis, and immunology, this textbook is well suited to aid both scientists and clinicians. The extensive illustrations, tables, and in-depth coverage of topics, including discussion of the non-infective aspects of allergic and toxin mediated fungal disease, are designed to aid the understanding of mechanisms and pathology, and extend the usual approach to fungal disease. This textbook is essential reading for microbiologists, research scientists, infectious diseases clinicians, respiratory physicians, and those managing immunocompromised patients. Part of the Oxford Textbook in Infectious Disease and Microbiology series, it is also a useful companion text for students and trainees looking to supplement mycology courses and microbiology training.
Author: Kary B. Mullis
Publisher: Springer Science & Business Media
ISBN: 1461202574
Category : Medical
Languages : en
Pages : 464
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Book Description
James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...
Author: Michael A. Innis
Publisher: Academic Press
ISBN: 0080919634
Category : Science
Languages : en
Pages : 589
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Book Description
PCR is the most powerful technique currently used in molecular biology. It enables the scientist to quickly replicate DNA and RNA on the benchtop. From its discovery in the early 80's, PCR has blossomed into a method that enables everything from ready mutation of DNA/RNA to speedy analysis of tens of thousands of nucleotide sequences daily.PCR Applications examines the latest developments in this field. It is the third book in the series, building on the previous publications PCR Protocols and PCR Strategies. The manual discusses techniques that focus on gene discovery, genomics, and DNA array technology, which are contributing factors to the now-occurring bioinformatics boom.Key Features* Focuses on gene discovery, genomics, and DNA array technology* Covers quantitative PCR techniques, including the use of standards and kinetic analysisincludes statistical refinement of primer design parameters* Ilustrates techniques used in microscopic tissue samples, such as single cell PCR, whole cell PCR, laser capture microdissection, and in situ PCREntries provide information on:* Nomenclature* Expression* Sequence analysis* Structure and function* Electrophysiology* Parmacology* Information retrieval
Author: Michael A. Innis
Publisher: Academic Press
ISBN: 008088671X
Category : Science
Languages : en
Pages : 501
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Book Description
The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. - Avoid contamination--with specific instructions on setting up your lab - Avoid cumbersome molecular biological techniques - Discover new applications
Author: Sarah Kidd
Publisher:
ISBN: 9780646951294
Category :
Languages : en
Pages :
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Book Description
Descriptions of Medical Fungi. Third Edition. Sarah Kidd, Catriona Halliday, Helen Alexiou and David Ellis. 2016. This updated third edition which includes new and revised descriptions. We have endeavoured to reconcile current morphological descriptions with more recent genetic data. More than 165 fungus species are described, including members of the Zygomycota, Hyphomycetes, Dimorphic Pathogens, Yeasts and Dermatophytes. 340 colour photographs. Antifungal Susceptibility Profiles. Microscopy Stains & Techniques. Specialised Culture Media. References. 250 pages.
Author:
Publisher:
ISBN: 9781913652357
Category :
Languages : en
Pages :
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Book Description
The editor and authors have produced an excellent book that will be extremely useful for all microbiologists. A recommended book for all microbiology laboratories.
