Microscopy, Immunohistochemistry, and Antigen Retrieval Methods PDF Download
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Author: M.A. Hayat
Publisher: Springer Science & Business Media
ISBN: 0306475995
Category : Medical
Languages : en
Pages : 368
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Book Description
Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.
Author: M.A. Hayat
Publisher: Springer Science & Business Media
ISBN: 0306475995
Category : Medical
Languages : en
Pages : 368
Get Book Here
Book Description
Histochemistry deals with the activities of chemical components in cells, and immunohistochemistry addresses the function of cell types in tissue or organs, such as those leading to acceptance or rejection of grafts or organs. This book is a methods volume focusing on antigen retrieval, particularly methods used in disease-related antigens. Because the book is a methods volume and a lab manual, it will have an audience of pathologists, biochemists, and lab technicians.
Author: Shuji Yamashita
Publisher:
ISBN:
Category : Medical
Languages : en
Pages : 0
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Book Description
Heat-induced antigen retrieval (HIAR) method reported by Shi et al. in 1991 has greatly contributed not only to immunohistochemistry but also to studying gene expressions using archived formalin-fixed and paraffin-embedded (FFPE) specimens. Heating cleaves crosslinks (methylene bridges) in formaldehyde-fixed proteins and extends polypeptides to expose epitopes hidden in the inner portion of antigens or covered by adjacent macromolecules. In this chapter, the following topics are described to reconsider the concept of immunohistochemistry flexibly and to apply HIAR for further immunological studies using a variety of specimens: (1) antigen-antibody interactions in tissues; (2) mechanisms of chemical fixation with formaldehyde, glutaraldehyde, and osmium tetroxide; (3) unmasking of epitopes using HIAR for specimens fixed with chemical fixatives, including highly masked epitopes; (4) a standardized fixative for immunoelectron microscopy-based HIAR; (5) HIAR for conventionally processed electron microscopy specimens embedded in epoxy resins; and (6) effects of antibody diluents on immunohistochemistry.
Author: M. A. Hayat
Publisher:
ISBN: 9781475776324
Category :
Languages : en
Pages : 384
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Book Description
Author: Igor B. Buchwalow
Publisher: Springer Science & Business Media
ISBN: 3642046096
Category : Medical
Languages : en
Pages : 153
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Book Description
This concise yet comprehensive guide to the methods and protocols of immunohistochemistry covers established techniques and current developments in the field such as the use of epitope tags, multiple immunolabeling and diagnostic immunohistochemistry.
Author: Shan-Rong Shi
Publisher: Eaton Publishing Company/Biotechniques Books
ISBN:
Category : Medical
Languages : en
Pages : 388
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Book Description
Author: Julia M. Polak
Publisher: Taylor & Francis
ISBN:
Category : Medical
Languages : en
Pages : 164
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Book Description
Immunocytochemical techniques are essential in diagnosis and biomedical research. This rewritten and updated introduction for beginners explains the reasons for the various steps, and discusses problems for specificity and sensitivity and their solutions. It includes practical instructions for several immunostaining methods using flourescent, enzyme and gold labels.
Author: Christopher Guerin
Publisher: Frontiers Media SA
ISBN: 283251605X
Category : Science
Languages : en
Pages : 161
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Book Description
Author: Charles Streckfus
Publisher: BoD – Books on Demand
ISBN: 1838808205
Category : Medical
Languages : en
Pages : 142
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Book Description
Immunohistochemistry - The Ageless Biotechnology is a book that is ideal for undergraduate and graduate biomedical researchers, and medical and dental health professionals. It is a detailed text, which emphasizes the laboratory and clinical implications of immunohistochemistry. The text covers the advances of immunohistochemistry from its humble origins in the 1930s up to the new decade of 2020. The book also offers a review of the immunohistochemistry detection systems with emphasis on their principles, history, and their advantages. It also stipulates the limitations and delineates the factors that need to be considered for choosing an appropriate detection system for IHC applications. The book describes current laboratory techniques and new applications for the technology. As the reader will observe, the book provides new and useful information concerning the rapidly advancing field of immunohistochemistry.
Author: Carlo Pellicciari
Publisher: Springer Nature
ISBN: 1071626752
Category : Science
Languages : en
Pages : 351
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Book Description
This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.
Author: Masashi Arita
Publisher: BoD – Books on Demand
ISBN: 1838818820
Category : Science
Languages : en
Pages : 102
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Book Description
TEM and SEM have contributed greatly to the progress of various research fields, which has been accelerated in the last few decades by highly functional electron microscopes and microscopy. In this tide of microscopy, various microscopic methods have been developed to make clear many unsolved problems, e.g. pulse beam TEM, environmental microscopy, correlative microscopy, etc. In this book, a number of reviews have been collected concerning these subjects. We think that the content in each chapter is impressive, and we hope this book will contribute to future advances in electron microscopy, materials science, and biomedicine.
